Volume 11, Issue 1 (4-2013)                   IJRM 2013, 11(1): 11-18 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bahadori M H, Ghasemian F, Ramezani M, Asgari Z. Melatonin effect during different maturation stages of oocyte and subsequent embryo development in mice. IJRM 2013; 11 (1) :11-18
URL: http://ijrm.ir/article-1-345-en.html
1- Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
2- Department of Biology, Biology Faculty, Kharazmi (Tarbiat Moallem) University, Tehran, Iran
3- Department of Biology, Faculty of Sciences, Ashtian Branch, Islamic Azad University, Ashtian, Iran
4- Department of Anatomy, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran , Asgari_bio@yahoo.com
Abstract:   (3194 Views)
Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger.
Objective: Effect of melatonin during in vitro oocyte maturation, fertilization and embryo development of mouse oocytes was evaluated.
Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cumulus oocyte complexes (COCs, group I) and denuded COC (d-COCs, group II). The oocytes were cultured in maturation medium with different doses of melatonin (1×101-105 nM). The cumulus expansion and nuclear status were evaluated after 24 h of in-vitro maturation. The oocytes were used for in-vitro fertilization. The fertilized oocytes were cultured in medium supplemented with different doses of melatonin.
Results: The expansion (86.79%) and maturation (80.55%) rate of COCs increased in supplemented medium with 10 nM of melatonin vs. control group (73.33%), p=0.006 and p=0.026 respectively), but oocytes without cumulus cells indicated higher maturation rate at higher melatonin doses (10 and 100 M, 84.34% and 79.5% respectively( vs. 69.33% in control group (p=0.002). Fertilization rate was higher in treated medium with 1 μM of melatonin (93.75%, p=0.007). The rate of cleavage and blastocyst formation was promoted in medium supplemented with 10 and 100 nM of melatonin (92.37% and 89.36% vs. 81.25% in control group, p=0.002). We observed a dose dependent response to melatonin treatment in this experiment.
Conclusion: Exogenous melatonin can promote cumulus cell expansion, in vitro oocyte maturation, and embryo development. However we investigated a dose-dependent response in different stages of maturation and development. It may reflect sensitive rate of oocytes and embryos to culture conditions.
Full-Text [PDF 534 kb]   (619 Downloads) |   |   Full-Text (HTML)  (380 Views)  
Type of Study: Original Article |

References
1. Agarwal A, Tamer MS, Mohamed AB, Jashoman B, Juan GA. Oxidative stressing assisted reproductive techniques. Fertil Steril 2006; 86: 503-512. [DOI:10.1016/j.fertnstert.2006.02.088]
2. Tamura H, Nakamura Y, Korkmaz A, Manchester LC, Tan DX, Sugino N, et al. Melatonin and the ovary: physiological and pathophysiological implications. Fertil Steril 2009; 92: 328-343. [DOI:10.1016/j.fertnstert.2008.05.016]
3. Polat MF, Taysi S, Gul M, Cikman O, Yilmaz I, Bakan E, et al. Oxidant/antioxidant status in blood of patients with malignant breast tumour and benign breast disease. Cell Biochem Func 2002; 20: 327-331. [DOI:10.1002/cbf.980]
4. Hardeland R. Antioxidative protection by melatonin: multiplicity of mechanisms from radical detoxification to radical avoidance. Endocrine 2005; 27: 119-130. [DOI:10.1385/ENDO:27:2:119]
5. Sharma S, Haldar C, Chaube SK. Effect of exogenous melatonin on X-ray induced cellular toxicity in lymphatic tissue of Indian tropical male squirrel, Funambulus pennanti. Int J Radiat Biol 2008; 84: 363-374. [DOI:10.1080/09553000802029894]
6. Ishizuka B, Kuribayashi Y, Murai K, Amemiya A, Itoh MT. The effect of melatonin on in vitro fertilization and embryo development in mice. J Pineal Res 2000; 28: 48-51. [DOI:10.1034/j.1600-079x.2000.280107.x]
7. Yeo CX, Gilchrist RB, Thompson JG, Lane Michelle. Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice. Hum Reprod 2008; 23: 67-73. [DOI:10.1093/humrep/dem140]
8. Koc M, Buyukokuroglu ME, Taysi S. The effect of melatonin on peripheral blood cells during total body irradiation in rats. Biol Pharm Bull 2002; 25: 656-657. [DOI:10.1248/bpb.25.656]
9. Reiter RJ, Herman TS, Meltz ML. Melatonin reduces gamma radiation-induced primary DNA damage in human blood lymphocytes. Mutat Res 1998; 397: 203-208. [DOI:10.1016/S0027-5107(97)00211-X]
10. Ballas LK, Elkin EB, Schrag D, Minsky BD, Bach PB. Radiation therapy facilities in the United States. Int J Radiat Oncol Biol Phys 2006; 66: 1204-1211. [DOI:10.1016/j.ijrobp.2006.06.035]
11. Kang IT, Koo OJ, Know DK, Park HJ, Jang G, Kang SK, Lee BC. Effects of melatonin on in vitro maturation of porcine oocyte and expression of melatonin receptor RNA in cumulus and granulosa cells. J Pineal Res 2009; 46; 22-28.
12. Takada L, Junior AM, Mingoti GZ, Balieiro JC, Cippola-Neto J, Coelhol A. Effects of melatonin on DNA damage of bovine cumulus cells during in vitro maturation (IVM) and on in vitro embryo development. Res Vet Sci 2012; 92: 124-127. [DOI:10.1016/j.rvsc.2010.11.004]
13. Tan DX, Manchester LC, Sainz RM, Mayo JC, Leon J, Hardeland R, et al. Interaction between melatonin and nicotinamide nucleotide. J Pineal Res 2005; 39: 185-194. [DOI:10.1111/j.1600-079X.2005.00234.x]
14. Reiter RJ, Tan DX, Maldonado MD. Melatonin as an antioxidant: physiology versus pharmacology. J Pineal Res 2005; 39: 215-216. [DOI:10.1111/j.1600-079X.2005.00261.x]
15. Nakamura Y, Tamura H, Takayama H, Kato H. Increased endogenous level of melatonin in preovulatory human follicles does not directly influence progesterone production. Fertil Steril 2003; 80: 1012-1016. [DOI:10.1016/S0015-0282(03)01008-2]
16. Murayama T, Kawashima M, Takahashi T, Yasuoka T, Kuwayama T, Tanaka K. Direct action of melatonin on hen ovarian granulose cells to lower responsiveness to luteinizing hormone. Proc Soc Exp Biol Med 1997; 215: 386-392. [DOI:10.3181/00379727-215-44148]
17. Tamura H, Nakamura Y, Takiguchi S, Kashida S, Yamagata Y, Sugino N, et al. Melatonin directly suppresses steroid production by preovulatory follicles in the cyclic hamster. J Pineal Res 1998; 25: 135-141. [DOI:10.1111/j.1600-079X.1998.tb00551.x]
18. Woo MM, Tai CJ, Kang SK, Nathwani PM, Pang SF, Leung PC. Direct action of melatonin in human granulose-luteal cells. J Clin Endocrinol Metab 2001; 86: 4789-4797. [DOI:10.1210/jcem.86.10.7912]
19. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3: 28. [DOI:10.1186/1477-7827-3-28]
20. Kowaltowski AJ, Vercesi AE. Mitochondrial damage induced by conditions of oxidative stress. Free Radic Biol Med 1999; 26: 463-471. [DOI:10.1016/S0891-5849(98)00216-0]
21. Noda Y, Matsumoto H, Umaoka Y, Tatsumi K, Kishi J, Mori T. Involvement of superoxide radicals in the mouse two-cell block. Mol Reprod Dev 1991; 28: 356-360. [DOI:10.1002/mrd.1080280408]
22. Ronnberg L, Kauppila A, Leppaluoto J, Martikainen H, Vakkuri O. Circadian and seasonal variation in human preovulatory follicular fluid melatonin concerntration. J Clin Endocrinol Metab 1990; 71: 492-496. [DOI:10.1210/jcem-71-2-493]
23. Adriaens I, Jacquet P, Cortvrindt R, Janssen K, Smitz J. Melatonin has does- dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis. Toxicology 2006; 228: 333-343. [DOI:10.1016/j.tox.2006.09.018]
24. Tokumoto T, Tokumoto M, Horiguchi R, Ishikawa K, Nagahama Y. Diethylstilbestrol induces fish oocyte maturation. Proc Natl Acad Sci USA 2004; 101: 3686-3690. [DOI:10.1073/pnas.0400072101]
25. Chattoraj A, Bhattacharyya S, Basu D, Bhattacharya S, Bhattacharya S, Maitra SK. Melatonin accelerates maturation inducing hormone (MIH): induced oocyte maturation in carps. Gen Comp Endocrinol 2005; 140: 145-155. [DOI:10.1016/j.ygcen.2004.10.013]
26. McElhinny AS, Davis FC, Warner CM. The effect of melatonin on cleavage rate of C57BL/6 and CBA/Ca preimplantation embryos cultured in vitro. J Pineal 1996; 21: 44-48. [DOI:10.1111/j.1600-079X.1996.tb00269.x]
27. Jahnke G, Marr M, Myers C, Wilson R, Travlos G, Price C. Maternal and developmental toxicity evaluation of melatonin administered orally to pregnant Sprague-Dawley rats. Toxicol Sci 1999; 50: 271-279.

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb