International Journal of
Reproductive Biomedicine
Tue, May 14, 2024
[Archive]
Volume 11, Issue 11 (12-2013)
IJRM 2013, 11(11): 875-0 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Eftekhar M, Janati S, Rahsepar M, Aflatoonian A. Effect of oocyte activation with calcium ionophore on ICSI outcomes in teratospermia: Randomized clinical trial. IJRM 2013; 11 (11) :875-0
URL: http://ijrm.ir/article-1-366-en.html
URL: http://ijrm.ir/article-1-366-en.html
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Sjanati@ymail.com
2- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Sjanati@ymail.com
Abstract: (2706 Views)
Background: Chemical activation is the most frequently used method for artificial oocyte activation (AOA), results in high fertilization rate.
Objective: This prospective, randomized, unblinded, clinical study aimed to evaluate the efficiency of oocyte activation with calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (ICSI) in infertile men suffer from teratoospermia.
Materials and Methods: Thirty eight women with teratoospermic partner underwent ICSI with antagonist protocol. A total of 313 metaphase II (MII) oocytes were randomly divided into two groups: In the oocytes of the control group (n=145), routine ICSI was applied. Oocytes in the AOA group (n=168) immediately after ICSI, were entered in culture medium supplemented with 5 μΜ calcium ionophore (A23187) for 5 minutes and then washed at least five times with MOPS solution. In both groups, the fertilization was evaluated 16-18 hours after ICSI.
Results: The number of fertilized oocytes and embryos obtained were significantly different between two groups (p=0.04). There was no significant difference between the two studied groups regarding the fertilization and cleavage rate (95.33% vs. 84.4%, p=0.11; and 89.56% vs. 87.74%, p=0.76, respectively). Implantation rate was higher in AOA group than in control group, but the difference was not significant (17.64% vs. 7.4%, p=0.14). No significant differences were observed in chemical and clinical pregnancy rate between groups (47.1% vs. 16.7%, p=0.07; and 41.2% vs. 16.7%; p=0.14, respectively).
Conclusion: We didn’t find significant difference in the implantation, fertilization, cleavage and pregnancy rates between the two groups but could significantly increase the number of fertilized oocytes and embryos obtained. Finally oocyte activation with calcium ionophore may improve ICSI outcomes in infertile men suffer from teratoospermia. Further study with more cases can provide greater value.
Objective: This prospective, randomized, unblinded, clinical study aimed to evaluate the efficiency of oocyte activation with calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (ICSI) in infertile men suffer from teratoospermia.
Materials and Methods: Thirty eight women with teratoospermic partner underwent ICSI with antagonist protocol. A total of 313 metaphase II (MII) oocytes were randomly divided into two groups: In the oocytes of the control group (n=145), routine ICSI was applied. Oocytes in the AOA group (n=168) immediately after ICSI, were entered in culture medium supplemented with 5 μΜ calcium ionophore (A23187) for 5 minutes and then washed at least five times with MOPS solution. In both groups, the fertilization was evaluated 16-18 hours after ICSI.
Results: The number of fertilized oocytes and embryos obtained were significantly different between two groups (p=0.04). There was no significant difference between the two studied groups regarding the fertilization and cleavage rate (95.33% vs. 84.4%, p=0.11; and 89.56% vs. 87.74%, p=0.76, respectively). Implantation rate was higher in AOA group than in control group, but the difference was not significant (17.64% vs. 7.4%, p=0.14). No significant differences were observed in chemical and clinical pregnancy rate between groups (47.1% vs. 16.7%, p=0.07; and 41.2% vs. 16.7%; p=0.14, respectively).
Conclusion: We didn’t find significant difference in the implantation, fertilization, cleavage and pregnancy rates between the two groups but could significantly increase the number of fertilized oocytes and embryos obtained. Finally oocyte activation with calcium ionophore may improve ICSI outcomes in infertile men suffer from teratoospermia. Further study with more cases can provide greater value.
Type of Study: Original Article |
References
1. Ramadan WM, Kashir J, Jones C, Coward K. Oocyte activation and phospholipase C zeta (PLCζ): diagnostic and therapeutic implications for assisted reproductive technology. Cell Commun Signal 2012; 10: 12. [DOI:10.1186/1478-811X-10-12]
2. Skakkebæk NE, Jørgensen N, Main KM, Meyts ERD, Leffers H, Andersson AM, et al. Is human fecundity declining? Int J Androl 2006; 29: 2-11. [DOI:10.1111/j.1365-2605.2005.00573.x]
3. Devroey P, Van Steirteghem A. A review of ten years experience of ICSI. Hum Reprod Update 2004; 10: 19-28. [DOI:10.1093/humupd/dmh004]
4. Nasr-Esfahani MH, Razavi S, Javdan Z, Tavalaee M. Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection. Fertil Steril 2008; 90: 2231-2237. [DOI:10.1016/j.fertnstert.2007.10.047]
5. Eftekhar M, Mohammadian F, Yousefnejad F, Khani P, Aflatoonian A. Effect of calcium ionophore on unfertilized oocytes after ICSI cycles. Iran J Reprod Med 2012; 10: 83-86.
6. Nasr-Esfahani MH, Deemeh MR, Tavalaee M. Artificial oocyte activation and intracytoplasmic sperm injection. Fertil Steril 2010; 94: 520-526. [DOI:10.1016/j.fertnstert.2009.03.061]
7. Flaherty SP, Payne D, Matthews CD. Fertilization failures and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod 1998; 13 (Suppl.): 155-164. [DOI:10.1093/humrep/13.suppl_1.155]
8. Nasr-Esfahani MH, Razavi S, Mardani M, Shirazi R, Javanmardi S. Effects of failed oocyte activation and sperm protamine deficiency on fertilization post-ICSI. Reprod Biomed Online 2007; 14: 422-429. [DOI:10.1016/S1472-6483(10)60888-7]
9. Sakkas D, Urner F, Bianchi P, Bizzaro D, Wagner I, Jaquenoud N, et al. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod 1996; 11: 837-843. [DOI:10.1093/oxfordjournals.humrep.a019263]
10. Tesarik J, Mendoza C, Greco E. The activity (calcium oscillator?) responsible for human oocyte activation after injection with round spermatids is associated with spermatid nuclei. Fertil Steril 2000; 74: 1245-1247. [DOI:10.1016/S0015-0282(00)01598-3]
11. Borges Jr E, de Almeida Ferreira Braga DP, de Sousa Bonetti TC, Iaconelli Jr A, Franco Jr JG. Artificial oocyte activation with calcium ionophore A23187 in intracytoplasmic sperm injection cycles using surgically retrieved spermatozoa. Fertil Steril 2009; 92: 131-136. [DOI:10.1016/j.fertnstert.2008.04.046]
12. Wang E, Taylor RW, Pfeiffer DR. Mechanism and specificity of lanthanide series cation transport by ionophores A23187, 4-BrA23187, and ionomycin. Biophys J 1998; 75: 1244-1254. [DOI:10.1016/S0006-3495(98)74044-5]
13. Kruger TF, Menkveld R, Stander F, Lombard C, Van der Merwe J, Van Zyl J, et al. Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil Steril 1986; 46: 1118-1123. [DOI:10.1016/S0015-0282(16)49891-2]
14. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 3rd Ed. Cambridge University Press, Cambridge; 1992.
15. Hill G, Freeman M, Bastias M, Rogers B, Herbert 3rd C, Osteen K, et al. The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertil Steril 1989; 52: 801-806. [DOI:10.1016/S0015-0282(16)61034-8]
16. Kasai T, Ogawa K, Mizuno K, Nagai S, Uchida Y, Ohta S, et al. Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential. Asian J Androl 2002; 4: 97-103.
17. Yamano S, Nakagawa K, Nakasaka H, Aono T. Fertilization failure and oocyte activation. J Med Invest 2000; 47: 1-8.
18. Battaglia DE, Koehler JK, Klein NA, Tucker MJ. Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm. Fertil Steril 1997; 68: 118-122. [DOI:10.1016/S0015-0282(97)81486-0]
19. Mansour R, Fahmy I, Tawab NA, Kamal A, El-Demery Y, Aboulghar M, et al. Electrical activation of oocytes after intracytoplasmic sperm injection: a controlled randomized study. Fertil Steril 2009; 91: 133-139. [DOI:10.1016/j.fertnstert.2007.08.017]
20. Lu Q, Zhao Y, Gao X, Li Y, Ma S, Mullen S, et al.
21. Combination of calcium ionophore A23187 with puromycin salvages human unfertilized oocytes after ICSI. Eur J Obstet Gynecol Reprod Biol 2006; 126: 72-76. [DOI:10.1016/j.ejogrb.2005.10.038]
22. Alberio R, Zakhartchenko V, Motlik J, Wolf E. Mammalian oocyte activation: lessons from the sperm and implications for nuclear transfer. Int J Dev Biol 2001; 45: 797-809.
23. Miao Y-L, Kikuchi K, Sun Q-Y, Schatten H. Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Hum Reprod Update 2009; 15: 573-585. [DOI:10.1093/humupd/dmp014]
24. Nagy ZP, Rienzi LF, Ubaldi FM, Greco E, Massey JB, Kort HI. Effect of reduced oocyte aging on the outcome of rescue intracytoplasmic sperm injection. Fertil Steril 2006; 85: 901-906. [DOI:10.1016/j.fertnstert.2005.09.029]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
