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Volume 11, Issue 9 (12-2013)
IJRM 2013, 11(9): 767-0 |
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Barkhordari A, Hekmatimoghaddam S, Jebali A, Khalili M A, Talebi A, Noorani M. Effect of zinc oxide nanoparticles on viability of human spermatozoa. IJRM 2013; 11 (9) :767-0
URL: http://ijrm.ir/article-1-459-en.html
URL: http://ijrm.ir/article-1-459-en.html
Abolfazl Barkhordari1 
, Seyedhossein Hekmatimoghaddam2 , Ali Jebali3 , Mohammad Ali Khalili4 , Alireza Talebi4 , Marzieh Noorani * 5
, Seyedhossein Hekmatimoghaddam2 , Ali Jebali3 , Mohammad Ali Khalili4 , Alireza Talebi4 , Marzieh Noorani * 5
1- Department of Occupational Health, School of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran
4- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5- Department of Occupational Health, School of Paramedicine, Abarkooh, Yazd, Iran , marzieh.noorani@gmail.com
2- Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3- Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran
4- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5- Department of Occupational Health, School of Paramedicine, Abarkooh, Yazd, Iran , marzieh.noorani@gmail.com
Abstract: (2793 Views)
Background: The extensive use of different nanoparticles has raised great concerns about their occupational and biological safety.
Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa.
Materials and Methods: Semen samples were obtained from 15 healthy persons, and were analyzed using WHO guidelines. Each semen sample was separately incubated with different concentrations of ZnO NPs (10, 100, 500, and 1000 μg/mL) at 37PoPC for 45, 90, and 180 minutes. Then, the cell death percentage of spermatozoa was measured by MTT assay. Mann-Whitney test was used for comparison of different times and concentrations.
Results: The maximum cell death percentage was 20.8%, 21.2%, and 33.2% after 45, 90, and 180 minutes, respectively. In case of concentration, the highest concentration (1000 μg/mL) of ZnO NPs led to the highest toxicity for all incubation times. Statistically, there were significant differences in cell viability after 180 minutes vs. 45 and 90 minutes.
Conclusion: This study indicated that cytotoxicity of ZnO NPs is dose and time dependent.
Objective: The aim of this study was to evaluate the cytotoxic effect of zinc oxide nanoparticles (ZnO NPs) on viability of spermatozoa.
Materials and Methods: Semen samples were obtained from 15 healthy persons, and were analyzed using WHO guidelines. Each semen sample was separately incubated with different concentrations of ZnO NPs (10, 100, 500, and 1000 μg/mL) at 37PoPC for 45, 90, and 180 minutes. Then, the cell death percentage of spermatozoa was measured by MTT assay. Mann-Whitney test was used for comparison of different times and concentrations.
Results: The maximum cell death percentage was 20.8%, 21.2%, and 33.2% after 45, 90, and 180 minutes, respectively. In case of concentration, the highest concentration (1000 μg/mL) of ZnO NPs led to the highest toxicity for all incubation times. Statistically, there were significant differences in cell viability after 180 minutes vs. 45 and 90 minutes.
Conclusion: This study indicated that cytotoxicity of ZnO NPs is dose and time dependent.
Type of Study: Original Article |
References
1. Jungwirth A, Giwercman A, Tournaye H, Diemer T, Kopa Z, Dohle G, et al. European Association of Urology Guidelines on Male Infertility: The 2012 Update. Eur Urol 2012; 62: 324-332. [DOI:10.1016/j.eururo.2012.04.048]
2. Peters K, Unger RE, Kirkpatrick CJ, Gatti AM, Monari E. Effects of nano-scaled particles on endothelial cell function in vitro: studies on viability, proliferation and inflammation. J Mater Sci Mater Med 2004; 15: 321-325. [DOI:10.1023/B:JMSM.0000021095.36878.1b]
3. Makhluf SBD, Qasem R, Rubinstein S, Gedanken A, Breitbart H. Loading magnetic nanoparticles into sperm cells does not affect their functionality. Langmuir 2006; 22: 9480-9482. [DOI:10.1021/la061988z]
4. Wiwanitkit V, Sereemaspun A, Rojanathanes R. Effect of gold nanoparticles on spermatozoa: the first world report. Fertil Steril 2009; 91: 7-8. [DOI:10.1016/j.fertnstert.2007.08.021]
5. Ema M, Kobayashi N, Naya M, Hanai S, Nakanishi J. Reproductive and developmental toxicity studies of manufactured nanomaterials. Reprod Toxicol 2010; 30: 343-352. [DOI:10.1016/j.reprotox.2010.06.002]
6. Philbrook NA, Winn LM, Afrooz AR, Saleh NB, Walker VK. The effect of TiO2 and Ag nanoparticles on reproduction and development of Drosophila melanogaster and CD-1 mice. Toxicol Appl Pharmacol 2011; 257: 429-436. [DOI:10.1016/j.taap.2011.09.027]
7. Kim YH, Fazlollahi F, Kennedy IM, Yacobi NR, Hamm-Alvarez SF, Borok Z, et al. Alveolar epithelial cell injury due to zinc oxide nanoparticle exposure. Am J Respir Crit Care Med 2010; 182: 1398-1409. [DOI:10.1164/rccm.201002-0185OC]
8. Heng BC, Zhao X, Xiong S, Ng KW, Boey FY, Loo JS. Toxicity of zinc oxide (ZnO) nanoparticles on human bronchial epithelial cells (BEAS-2B) is accentuated by oxidative stress. Food Chem Toxicol 2010; 48: 1762-1766. [DOI:10.1016/j.fct.2010.04.023]
9. Zvekić D, Srdić VV, Karaman MA, Matavulj MN. Antimicrobial properties of ZnO nanoparticles incorporated in polyurethane varnish. Proc Appl Ceram 2011; 5: 41-45. [DOI:10.2298/PAC1101041Z]
10. World Health Organization. WHO laboratory manual for the examination and processing of human semen: World Health Organization; 2010.
11. Slater TF, Sawyer B, Sträeuli U. Studies on succinate-tetrazolium reductase systems. III. Points of coupling of four different tetrazolium salts. Biochim Biophys Acta 1963; 77: 383-393. [DOI:10.1016/0006-3002(63)90513-4]
12. Iqbal M, Ijaz A, Aleem M, Rehman H, Yousaf MS. Assessment of Nili-Ravi buffalo (Bubalus bubalis) semen by MTT reduction assay. South Afr J Anim Sci 2009; 39: 294-300.
13. Sahoo SK, Parveen S, Panda JJ. The present and future of nanotechnology in human health care. Nanomedicine 2007; 3: 20-31. [DOI:10.1016/j.nano.2006.11.008]
14. Anerao AM, Sharma RC, Rathore Mansee, Gangawane AK. Studies on human sperm motility and viability when treatment with rock salt. J Pathol Res 2010; 1: 1-10.
15. Rurangw E, Kime DE, Ollevier F, Nash JP. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture 2004; 234: 1-28. [DOI:10.1016/j.aquaculture.2003.12.006]
16. Nasr-Esfahani MH, Aboutorabi R, Esfandiari E, Mardani M. Sperm MTT viability assay: a new method for evaluation of human sperm viability. J Assist Reprod Genet 2002; 19: 477-482. [DOI:10.1023/A:1020310503143]
17. Gaczarzewicz D, Piasecka M, Udala J, Blaszczyk B, Laszczynska M, Kram A. Oxidoreductive capability of boar sperm mitochondria in fresh semen and during their preservation in BTS extender. Reprod Biol 2003; 3: 161-172.
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