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Reproductive Biomedicine
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Volume 12, Issue 10 (11-2014)
IJRM 2014, 12(10): 705-0 |
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Mehraban F, Jafari M, Akbartabar Toori M, Sadeghi H, Joodi B, Mostafazade M et al . Effects of date palm pollen (Phoenix dactylifera L.) and Astragalus ovinus on sperm parameters and sex hormones in adult male rats. IJRM 2014; 12 (10) :705-0
URL: http://ijrm.ir/article-1-480-en.html
URL: http://ijrm.ir/article-1-480-en.html
Fouad Mehraban1 
, Mehrzad Jafari2 , Mehdi Akbartabar Toori3 , Hossein Sadeghi1 , Behzad Joodi4 , Mostafa Mostafazade4 , Heibatollah Sadeghi * 5
, Mehrzad Jafari2 , Mehdi Akbartabar Toori3 , Hossein Sadeghi1 , Behzad Joodi4 , Mostafa Mostafazade4 , Heibatollah Sadeghi * 5
1- Medicinal Plant Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2- Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
3- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
4- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
5- Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran ,Sadeghi.ha@yums.ac.ir
2- Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
3- Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
4- Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran
5- Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran ,
Abstract: (6059 Views)
Background: Date Palm Pollen (DPP) and Astragalus genus are used in some countries for the treatment of infertility.
Objective: This study was designed to investigate effects of DPP and Astragalus ovinus (A.Ovinus) on fertility in healthy adult male rats.
Materials and Methods: Thirty-six rats were divided into six groups (n=6) including control and five treatment groups. DPP (120, 240 and 360 mg/kg) and A.ovinus (100, 500 mg/ kg) were orally given to the treatment groups. After thirty-five days, blood samples were taken to determine serum levels of FSH, LH, testosterone and estradiol. Weight of testis and epididymis, sperm count, sperm motility, seminiferous tubules diameter (STD), germinal cell layer thickness (GCLT), sertoli, leydig and spermatogonia cells were also evaluated.
Results: DPP at the of 120 and 240 mg/kg doses significantly raised the ratio of testis or epididymis to body weight, sperm count, sperm motility , and estradiol level compared to the control group (p<0.05). LH and testosterone levels only noticeably increased at 120 mg/kg of DPP (p<0.01 and p<0.001 respectively). STD increased in the three applied doses (p=0.001). A. ovinus extract at the indicated doses produced a significant reduction in the ratio of testis or epididymis to body weight and sperm motility (p<0.05). Sperm count, spermatogonia, leydig cells and FSH level decreased at dose of 500 mg/kg. Furthermore, GCLT, spermatogonia cells, and serum estradiol level increased at 100 mg/kg dose of A. ovinus.
Conclusion: Our findings indicate that DPP could improve fertility factors, while A.ovinus can exhibit deleterious effects on gonad and sperm parameters in rats.
Objective: This study was designed to investigate effects of DPP and Astragalus ovinus (A.Ovinus) on fertility in healthy adult male rats.
Materials and Methods: Thirty-six rats were divided into six groups (n=6) including control and five treatment groups. DPP (120, 240 and 360 mg/kg) and A.ovinus (100, 500 mg/ kg) were orally given to the treatment groups. After thirty-five days, blood samples were taken to determine serum levels of FSH, LH, testosterone and estradiol. Weight of testis and epididymis, sperm count, sperm motility, seminiferous tubules diameter (STD), germinal cell layer thickness (GCLT), sertoli, leydig and spermatogonia cells were also evaluated.
Results: DPP at the of 120 and 240 mg/kg doses significantly raised the ratio of testis or epididymis to body weight, sperm count, sperm motility , and estradiol level compared to the control group (p<0.05). LH and testosterone levels only noticeably increased at 120 mg/kg of DPP (p<0.01 and p<0.001 respectively). STD increased in the three applied doses (p=0.001). A. ovinus extract at the indicated doses produced a significant reduction in the ratio of testis or epididymis to body weight and sperm motility (p<0.05). Sperm count, spermatogonia, leydig cells and FSH level decreased at dose of 500 mg/kg. Furthermore, GCLT, spermatogonia cells, and serum estradiol level increased at 100 mg/kg dose of A. ovinus.
Conclusion: Our findings indicate that DPP could improve fertility factors, while A.ovinus can exhibit deleterious effects on gonad and sperm parameters in rats.
Type of Study: Original Article |
References
1. Shefi S, Turek PJ, Shai Shefi PJT. Definition and Current Evaluation of Subfertile Men. Int Braz J Urol 2006; 32: 385-397. [DOI:10.1590/S1677-55382006000400002]
2. Raman JD, Nobert CF, Goldstein M. Increased incidence of testicular cancer in men presenting with infertility and abnormal semen analysis. J Urol 2005; 174: 1819-1822. [DOI:10.1097/01.ju.0000177491.98461.aa]
3. Kolettis Pn. Evaluation of the Subfertile Man. Am Fam Physisan 2003; 67: 2165-2172.
4. Marah I, Marbeen AEA-S, Mossa M, Marbut I, Allahwerdy Y. The probable therapeutic effects of date palm pollen in the treatment of male infertility. Tikrit J Pharmaceutical Sci 2005; 1: 30-35.
5. Sharpe RM, Franks S. Environment, lifestyle and infertility: an inter-generational issue. Nat Cell Biol 2002: 4 (Suppl.): 33-40.
6. Bin-Seng Low P, Kit-Lam C. Standardized quassinoid-rich Eurycoma longifolia extract improved spermatogenesis and fertility in male rats via the hypothalamic- pituitary- gonadalaxis. J Ethnopharmacol 2013; 145: 706-714. [DOI:10.1016/j.jep.2012.11.013]
7. El-Neweshy MS, ZKE-M YS. El-Sayed. Therapeutic effects of date palm (Phoenix dactylifera L.) pollen extract on cadmium-induced testicular toxicity. Andrologia 2013; 45: 369-378. [DOI:10.1111/and.12025]
8. Kim W, Kim SH, Park SK, Chang MS. Astragalus membranaceus ameliorates reproductive toxicity induced by cyclophosphamide in male mice. Phytother Res 2012; 26: 1418-1421. [DOI:10.1002/ptr.4756]
9. Liu J, Liang P, Yin C, Wang T, Li H, Li Y, et al. Effects of several Chinese herbal aqueous extracts on human sperm motility in vitro. Andrologia 2004; 36: 78-83. [DOI:10.1111/j.1439-0272.2004.00607.x]
10. Hong CY, Ku J, Wu P. Astragalus membranaceus stimulates human sperm motility in vitro. Am J Chinese Med 1992; 20: 289-294. [DOI:10.1142/S0192415X92000308]
11. Yakubu MT AM, Oladiji AT. Male sexual dysfunction and methods used in assessing medicinal plants with aphrodisiac potentials. Pharmacogn Rev 2007; 1: 49-56.
12. Hassan WA, El-kashlan AM, Ehssan NA. Egyptian Date Palm Pollen Ameliorates Testicular Dysfunction Induced by Cadmium Chloride in Adult Male Rats. J Am Sci 2012; 8: 659-669.
13. Nantia EAPFM, Monsees TK, Carreau S. Medicinal plants as potential male anti-infertility agents: a review. Andrologia 2009; 19: 148-158.
14. Mahran GH A-WS, Attia AM. A phyto-chemical study of date palm pollen. Planta Med 1976; 29: 171-175. [DOI:10.1055/s-0028-1097648]
15. Bennet RD KS, Heftmann E. Isolation of estrone and cholesterol from the date palm Phoenix dactylifera. Phytochem 1966; 5: 231-235. [DOI:10.1016/S0031-9422(00)85122-5]
16. Bahmanpour S, Talaei T, Vojdani Z, Panjehshahin MR, Poostpasand LA, Zareei S, et al. Effect of Phoenix dactylifera pollen on sperm parameters and reproductive system of adult male rats. Iran J Med Sci 2006; 31: 208-212.
17. Frodin DG. History and concepts of big plant genera. Taxon 2004: 53: 753-776.
18. R´ıos JL PGW. A Review of the Pharmacology and Toxicology of Astragalus. Phytother Res 1997; 11: 411-418.
https://doi.org/10.1002/(SICI)1099-1573(199709)11:6<411::AID-PTR132>3.0.CO;2-6 [DOI:10.1002/(SICI)1099-1573(199709)11:63.0.CO;2-6]
19. Abedi A, Parviz M, Karimian SM, Sadeghipour Rodsari HR. The Effect of Aqueous Extract of Phoenix Dactylifera Pollen Grain on Sexual Behavior of Male Rats. J Phys Pharm Adv 2012; 2: 235-242.
20. Schroder JD. Reproduction and Breeding Techniques for Laboratory Animals. Can J Comp Med 1971; 35: 345.
21. XIE BG, LI J, ZHU WJ. Pathological changes of testicular tissue in normal adult mice: A retrospective analysis. Exp Ther Med 2014; 7: 654-656. [DOI:10.3892/etm.2014.1481]
22. Jafari Barmak M, Khaksar Z. [Effect of Aloe Vera Extract on Testicular Tissue of Emberyo of Diabetic Rats]. Armaghan Danesh 2012; 17: 149-155. (In Persian)
23. Bearden HJ, Fuquay JW. Semen evaluation. Appl Anim Reprod 1997; 4: 159-170.
24. Liang P, Li H, Peng X, Xiao J, Liu J, Ye Z. [Effects of astragalus membranaceus injection on sperm abnormality in Cd-induced rats]. Zhonghua nan ke xue Natl J Androl 2004; 10: 42. (In Chinese)
25. Akunna GG, Saalu LC, Ogunlade B. Tackling Infertility with Medicinal Plant: Another Instance. Glob J Med Plant Res 2013; 1: 93-105.
26. Turk G, Sonmez M, Aydin M, Yuce A, Gur S, Yuksel M, et al. Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin Nutr 2008; 27: 289. [DOI:10.1016/j.clnu.2007.12.006]
27. Tahtamouni LH, Alqurna NM, Al-Hudhud MY, Al-Hajj HA. Dandelion Taraxacum officinale decreases male rat fertility in vivo. J Ethnopharmacol 2011; 135: 102-109. [DOI:10.1016/j.jep.2011.02.027]
28. Mali PC, Ansari AS, Chaturvedi M. Antifertility effect of chronically administeredMartynia annua root extract on male rats. J Ethnopharmacol 2002; 82: 61-67. [DOI:10.1016/S0378-8741(02)00084-3]
29. Shukla S, Mathur R, Prakash AO. Antifertility profile of the aqueous extract of Moringa oleifera roots. J Ethnopharmacol 1988; 22: 51-62. [DOI:10.1016/0378-8741(88)90230-9]
30. James LF, Molyneux RJ, Panter KE. The potential for the toxic principles of Astragalus and related plants to appear in meat and milk. Vet Hum Toxicol 1990; 32: 104.
31. Yakubu MT, Akanji MA, Oladiji AT, Adesokan AA. Androgenic potentials of aqueous extract of Massularia acuminata (G. Don) Bullock ex Hoyl. stem in male Wistar rats. J Ethnopharmacol 2008; 118: 508-513. [DOI:10.1016/j.jep.2008.05.020]
32. Bawazir AE. Investigations on the Chronic Effect of Talbina (Barly Water) on Hormone (Cortisol and Testosterone), Reproductive System and Some Neurotransmitter. Am-Euras J Sci Res 2010; 5: 134-142.
33. Adimoelja A. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions. Int J Androl 2000; 23: 82-84. [DOI:10.1046/j.1365-2605.2000.00020.x]
34. Elberry AA, Mufti ST, Al-Maghrabi JA, Abdel-Sattar EA, Ashour OM, Ghareib SA and Mosli HA. Anti-inflammatory and antiproliferative activities of date palm pollen (Phoenix dactylifera) on experimentally-induced atypical prostatic hyperplasia in rats. J Inflamm 2011, 8: 1-13.
35. Yakubu MT, Afolayan AJ. Reproductive toxicologic evaluations of Bulbine natalensis Baker stem extract in albino rats. Theriogenology 2009; 72: 322-332. [DOI:10.1016/j.theriogenology.2009.01.026]
36. Tarazona JV, Sanz F. Aliphatic nitro compounds in Astragalus lusitanicus Lam. Vet Hum Toxicol 1987; 29: 437.
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