Volume 16, Issue 3 (March 2018)                   IJRM 2018, 16(3): 183-190 | Back to browse issues page

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Zohour Soleimani M, Jalali Mashayekhi F, Mousavi Hasanzade M, Baazm M. Alteration in CatSper1 and 2 genes expression, sperm parameters and testis histology in varicocelized rats. IJRM 2018; 16 (3) :183-190
URL: http://ijrm.ir/article-1-1030-en.html
Alteration in CatSper1 and 2 genes expression, sperm parameters and testis histology in varicocelized rats
Maryam Zohour Soleimani1 , Farideh Jalali Mashayekhi2 , Morteza Mousavi Hasanzade1 , Maryam Baazm * 3
1- Students Research Committee, Arak University of Medical Sciences, Arak, Iran
2- Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
3- Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran. , Dr.baazm@arakmu.ac.ir
Abstract:   (3568 Views)
Background: CatSper gene, a member of cation channel sperm family, has an essential role in sperm motility and male fertility. Following varicocele, sperm parameters especially sperm movement decreases. For this reason, we hypothesized that CatSper gene expression might be reduced after varicocele induction in an animal model.
Objective: The aim of this study was to evaluate the expression of CatSper 1 and 2 genes, sperm parameters and testis histology following varicocele induction.
Materials and Methods: A total of 30 Wistar male rats were randomly divided into three following groups (n=10/ each): control, sham, and varicocele group. Experimental varicocele was induced by partial ligation of the left renal vein. The epididymal sperm parameters, CatSper1 and 2 genes expression, and testes histology were studied two months after varicocele induction.
Results: Our results revealed that motility (32.73±16.14%), morphology (48.80±17%) and viability (31.23±9.82%) of sperms significantly reduced following varicocele induction. In addition, we showed a significant decrease in the number of spermatogonia (43.63±5.31) and seminiferous tubules diameters (190.51±19.23 mm) in experimental varicocele rats. The level of CatSper1 and 2 genes expression evaluated using real-time polymerase chain reaction was significantly downregulated 2 months after varicocele induction.
Conclusion: Our data indicated that experimental varicocele has deleterious effects on sperm parameters, testis structure as well as the expression of CatSper 1 and 2 genes.
Keywords: Varicocele, CatSper, Sperm parameters, Rat.
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Type of Study: Original Article |
References
1. Agarwal A, Deepinder F, Cocuzza M, Agarwal R, Short RA, Sabanegh E, et al. Efficacy of varicocelectomy in improving semen parameters: new meta-analytical approach. Urology 2007; 70: 532-538. [DOI:10.1016/j.urology.2007.04.011]
2. Witt MA, Lipshultz LI. Varicocele: a progressive or static lesion? Urology 1993; 42: 541-543. [DOI:10.1016/0090-4295(93)90268-F]
3. Pastuszak AW, Wang R. Varicocele and testicular function. Asian J Androl 2015; 17: 659-667. [DOI:10.4103/1008-682X.153539]
4. Marmar JL. The pathophysiology of varicoceles in the light of current molecular and genetic information. Hum Reprod Update 2001; 7: 461-472. [DOI:10.1093/humupd/7.5.461]
5. Mohammed A, Chinegwundoh F. Testicular varicocele: an overview. Urol Int 2009; 82: 373-379. [DOI:10.1159/000218523]
6. Luo DY, Yang G, Liu JJ, Yang YR, Dong Q. Effects of varicocele on testosterone, apoptosis and expression of StAR mRNA in rat Leydig cells. Asian J Androl 2011; 13: 287-291. [DOI:10.1038/aja.2010.111]
7. Mostafa T, Anis TH, El‐Nashar A, Imam H, Othman IA. Varicocelectomy reduces reactive oxygen species levels and increases antioxidant activity of seminal plasma from infertile men with varicocele. Int J Androl 2001; 24: 261-265. [DOI:10.1046/j.1365-2605.2001.00296.x]
8. Santoro G, Romeo C, Impellizzeri P, Ientile R, Cutroneo G, Trimarchi F, et al. Nitric oxide synthase patterns in normal and varicocele testis in adolescents. BJU Int 2001; 88: 967-973. [DOI:10.1046/j.1464-4096.2001.02446.x]
9. Köksal T, Erdoğru T, Toptaş B, Gülkesen KH, Usta M, Baykal A, et al. Effect of experimental varicocele in rats on testicular oxidative stress status. Andrologia 2002; 34: 242-247. [DOI:10.1046/j.1439-0272.2002.00500.x]
10. Turner T. The study of varicocele through the use of animal models. Hum Reprod Update 2001; 7: 78-84. [DOI:10.1093/humupd/7.1.78]
11. Mormandi E, Levalle O, Ballerini MG, Hermes R, Calandra RS, Campo S. Serum levels of dimeric and monomeric inhibins and the degree of seminal alteration in infertile men with varicocele. Andrologia 2003; 35: 106-111. [DOI:10.1046/j.1439-0272.2003.00546.x]
12. Darszon A, Labarca P, Nishigaki T, Espinosa F. Ion channels in sperm physiology. Physiol Rev 1999; 79: 481-510. [DOI:10.1152/physrev.1999.79.2.481]
13. Gagnon C. Controls of Serm Motility: Biological and Clinical Aspects: CRC Press; 1990.
14. Kirichok Y, Navarro B, Clapham DE. Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel. Nature 2006; 439: 737-740. [DOI:10.1038/nature04417]
15. Carlson AE, Westenbroek RE, Quill T, Ren D, Clapham DE, Hille B, et al. CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm. Proc Nati Acad Sci USA 2003; 100: 14864-14868. [DOI:10.1073/pnas.2536658100]
16. Qi H, Moran MM, Navarro B, Chong JA, Krapivinsky G, Krapivinsky L, et al. All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility. Proc Nati Acad Sci USA 2007; 104: 1219-1223. [DOI:10.1073/pnas.0610286104]
17. Suarez SS. Control of hyperactivation in sperm. Hum Reprod Update 2008; 14: 647-657. [DOI:10.1093/humupd/dmn029]
18. Ren D, Navarro B, Perez G, Jackson AC, Hsu S, Shi Q, et al. A sperm ion channel required for sperm motility and male fertility. Nature 2001; 413: 603-609. [DOI:10.1038/35098027]
19. Quill TA, Sugden SA, Rossi KL, Doolittle LK, Hammer RE, Garbers DL. Hyperactivated sperm motility driven by CatSper2 is required for fertilization. Proc Nati Acad Sci USA 2003; 100: 14869-14874. [DOI:10.1073/pnas.2136654100]
20. Askari Jahromi M, Movahedin M, Mazaheri Z, Amanlu M, Mowla SJ, Batooli H. Evaluating the effects of Escanbil (Calligonum) extract on the expression level of Catsper gene variants and sperm motility in aging male mice. Iran J Reprod Med 2014; 12: 459-466.
21. Rezaian J, Movahedin M, Mowla SJ. CatSper genes expression, semen characteristics and histology of testes in the contusive spinal cord-injured mice model. Spinal Cord 2009 47: 76-81. [DOI:10.1038/sc.2008.81]
22. Wang HF, Liu M, Li N, Luo T, Zheng LP, Zeng XH. Bisphenol a lmpairs mature sperm functions by a catSper-relevant mechanism. Toxicol Sci 2016; 152: 145-154. [DOI:10.1093/toxsci/kfw070]
23. Amini M, Shirinbayan P, Behnam B, Roghani M, Farhoudian A, Joghataei M, et al. Correlation between expression of CatSper family and sperm profiles in the adult mouse testis following Iranian Kerack abuse. Andrology 2014; 2: 386-393. [DOI:10.1111/j.2047-2927.2014.00195.x]
24. Jahnke VE, Van Der Meulen JH, Johnston HK, Ghimbovschi S, Partridge T, Hoffman EP, et al. Metabolic remodeling agents show beneficial effects in the dystrophin-deficient mdx mouse model. Skelet Muscle 2012; 2: 16. [DOI:10.1186/2044-5040-2-16]
25. Nasr‐Esfahani MH, Abasi H, Razavi S, Ashrafi S, Tavalaee M. Varicocelectomy: semen parameters and protamine deficiency. Int J Androl 2009; 32: 115-122. [DOI:10.1111/j.1365-2605.2007.00822.x]
26. Tajaddini S, Ebrahimi S, Behnam B, Bakhtiyari M, Joghataei MT, Abbasi M, et al. Antioxidant effect of manganese on the testis structure and sperm parameters of formalin‐treated mice. Andrologia 2014; 46: 246-253. [DOI:10.1111/and.12069]
27. Mohammadnejad D, Abedelahi A, Rashtbar M. Protective role of GnRH antagonist on chemotherapy-induced spermatogenesis disorder: A morphological study. Adv Pharm Bull 2013; 3: 323-328.
28. Haron MN, D'Souza UJ, Jaafar H, Zakaria R, Singh HJ. Exogenous leptin administration decreases sperm count and increases the fraction of abnormal sperm in adult rats. Fertil Steril 2010; 93: 322-324. [DOI:10.1016/j.fertnstert.2009.07.995]
29. Chen SR, Batool A, Wang YQ, Hao XX, Chang CS, Cheng CY, et al. The control of male fertility by spermatid-specific factors: searching for contraceptive targets from spermatozoon's head to tail. Cell Death Dis 2016; 7: e2472. [DOI:10.1038/cddis.2016.344]
30. Nikpoor P, Mowla SJ, Movahedin M, Ziaee SA, Tiraihi T. CatSper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility. Hum Reprod 2004; 19: 124-128. [DOI:10.1093/humrep/deh043]
31. Ren D, Xia J. Calcium signaling through CatSper channels in mammalian fertilization. Physiology 2010; 25: 165-175. [DOI:10.1152/physiol.00049.2009]
32. Pereira R, Sá R, Barros A, Sousa M. Major regulatory mechanisms involved in sperm motility. Asian J Androl 2017; 19: 5-14.
33. Sun XH, Zhu YY, Wang L, Liu Hl, Ling Y, Li Zl, et al. The Catsper channel and its roles in male fertility: a systematic review. Reprod Biol Endocrinol 2017; 15: 65. [DOI:10.1186/s12958-017-0281-2]
34. Singh AP, Rajender S. CatSper channel, sperm function and male fertility. Reprod Biomed Online 2015; 30: 28-38. [DOI:10.1016/j.rbmo.2014.09.014]
35. Mohammadi S, Movahedin M, Mowla SJ. Up-regulation of CatSper genes family by selenium. Reprod Biol Endocrinol 2009; 7: 126. [DOI:10.1186/1477-7827-7-126]
36. Mannowetz N, Miller MR, Lishko PV. Regulation of the sperm calcium channel CatSper by endogenous steroids and plant triterpenoids. Proc Nati Acad Sci 2017; 114: 5743-5748. [DOI:10.1073/pnas.1700367114]
37. Mancini A, Milardi D, Conte G, Festa R, De Marinis L, Littarru GP. Seminal antioxidants in humans: preoperative and postoperative evaluation of coenzyme Q10 in varicocele patients. Horm Metab Res 2005; 37: 428-432. [DOI:10.1055/s-2005-870232]
38. Balercia G, Arnaldi G, Fazioli F, Serresi M, Alleva R, Mancini A, et al. Coenzyme Q10 levels in idiopathic and varicocele‐associated asthenozoospermia. Andrologia 2002; 34: 107-111. [DOI:10.1046/j.0303-4569.2001.00485.x]
39. Knudson G, Ross L, Stuhldreher D, Houlihan D, Bruns E, Prins G. Prevalence of sperm bound antibodies in infertile men with varicocele: the effect of varicocele ligation on antibody levels and semen response. J Urol 1994; 151: 1260-1262. [DOI:10.1016/S0022-5347(17)35226-6]
40. Djaladat H, Mehrsai A, Rezazade M, Djaladat Y, Pourmand G. Varicocele and antisperm antibody: fact or fiction? South Med J 2006; 99: 44-47. [DOI:10.1097/01.smj.0000197036.08282.70]
41. Pasqualotto FF, Lucon AM, de Góes PM, Sobreiro BP, Hallak J, Pasqualotto EB, et al. Semen profile, testicular volume, and hormonal levels in infertile patients with varicoceles compared with fertile men with and without varicoceles. Fertil Steril 2005; 83: 74-77. [DOI:10.1016/j.fertnstert.2004.06.047]
42. Shiraishi K, Matsuyama H, Takihara H. Pathophysiology of varicocele in male infertility in the era of assisted reproductive technology. Int J Urol 2012; 19: 538-550. [DOI:10.1111/j.1442-2042.2012.02982.x]
43. Barqawi A, Caruso A, Meacham RB. Experimental varicocele induces testicular germ cell apoptosis in the rat. J Urol 2004; 171: 501-503. [DOI:10.1097/01.ju.0000088775.69010.61]
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