International Journal of
Reproductive Biomedicine
Sun, Dec 22, 2024
[Archive]
Volume 18, Issue 6 (June 2020)
IJRM 2020, 18(6): 449-464 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Keshavarzi B, Tabatabaei M, Zarnani A H, Ramezani Tehrani F, Bozorgmehr M, Mosaffa N. The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study. IJRM 2020; 18 (6) :449-464
URL: http://ijrm.ir/article-1-1077-en.html
URL: http://ijrm.ir/article-1-1077-en.html
Bahare Keshavarzi1 
, Meraj Tabatabaei1 , Amir Hasan Zarnani2 , Fahime Ramezani Tehrani3 , Mahmood Bozorgmehr2 , Nariman. Mosaffa * 4
, Meraj Tabatabaei1 , Amir Hasan Zarnani2 , Fahime Ramezani Tehrani3 , Mahmood Bozorgmehr2 , Nariman. Mosaffa * 4
1- Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
3- Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ,mosaffan@sbmu.ac.ir
2- Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
3- Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. ,
Abstract: (2611 Views)
Background: The amniotic membrane plays an important role in maintaining a healthy pregnancy. The main population cells from amniotic membrane include human amnion epithelial cells (hAECs) which have been shown to possess immunomodulatory properties.
Objective: The proximity of hAECs with monocyte leads to the generation of tollerogenic dendritic cells.
Materials and Methods: hAECs were obtained from normal pregnancy. Peripheral blood monocytes were isolated by anti-CD14 MACS method. Co-cultures of monocytes and hAECs were established in Transwell chambers supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) in the absence and presence of lipopolysaccharide (LPS) to produce immature and mature DCs, respectively. Immunophenotyping of the obtained DCs was done through flow cytometry and the production of cytokines was measured by ELISA. Mixed leukocyte Reaction (MLR) was also performed for the functional assessment of DCs.
Results: Immunophenotyping of [hAECs - Immature DC (iDC)] and [hAECs - iDC] + LPS cells revealed that the expression of CD1a, CD80, CD86, CD40, HLA-DR, and CD83 markers showed no significant difference as compared with the control group (iDCs and mDCs alone). In the [hAECs-iDCs] + LPS cells, the percentage of CD14 cells at the ratio of 1:2.5 showed significant differences compared to the control group. The production of IL-10 and IL-12 showed no significant difference in any of the cultures as compared to the control groups. Also, co-cultured DCs did not inhibit proliferation of lymphocyte.
Conclusion: Our findings show that factors secreted from cultured hAECs are unable to generate of tollerogenic dendritic cells. To achieve a better understanding of other mechanisms more investigations are needed.
Objective: The proximity of hAECs with monocyte leads to the generation of tollerogenic dendritic cells.
Materials and Methods: hAECs were obtained from normal pregnancy. Peripheral blood monocytes were isolated by anti-CD14 MACS method. Co-cultures of monocytes and hAECs were established in Transwell chambers supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) in the absence and presence of lipopolysaccharide (LPS) to produce immature and mature DCs, respectively. Immunophenotyping of the obtained DCs was done through flow cytometry and the production of cytokines was measured by ELISA. Mixed leukocyte Reaction (MLR) was also performed for the functional assessment of DCs.
Results: Immunophenotyping of [hAECs - Immature DC (iDC)] and [hAECs - iDC] + LPS cells revealed that the expression of CD1a, CD80, CD86, CD40, HLA-DR, and CD83 markers showed no significant difference as compared with the control group (iDCs and mDCs alone). In the [hAECs-iDCs] + LPS cells, the percentage of CD14 cells at the ratio of 1:2.5 showed significant differences compared to the control group. The production of IL-10 and IL-12 showed no significant difference in any of the cultures as compared to the control groups. Also, co-cultured DCs did not inhibit proliferation of lymphocyte.
Conclusion: Our findings show that factors secreted from cultured hAECs are unable to generate of tollerogenic dendritic cells. To achieve a better understanding of other mechanisms more investigations are needed.
Type of Study: Original Article |
Subject:
Reproductive Biology
References
1. Calvin SE, Oyen ML. Microstructure and mechanics of the chorioamnion membrane with an emphasis on fracture properties. Ann N Y Acad Sci 2007; 1101: 166-185. [DOI:10.1196/annals.1389.009] [PMID]
2. Li H, Niederkorn JY, Neelam S, Mayhew E, Word RA, McCulley JP, et al. Immunosuppressive factors secreted by human amniotic epithelial cells. Invest Ophthalmol Vis Sci. 2005; 46: 900-907. [DOI:10.1167/iovs.04-0495] [PMID]
3. Hori J, Wang M, Kamiya K, Takahashi H, Sakuragawa N. Immunological characteristics of amniotic epithelium. Cornea 2006; 25: S53-S58. [DOI:10.1097/01.ico.0000247214.31757.5c] [PMID]
4. Branski LK, Herndon DN, Celis MM, Norbury WB, Masters OE, Jeschke MG. Amnion in the treatment of pediatric partial-thickness facial burns. Burns 2008; 34: 393-399. [DOI:10.1016/j.burns.2007.06.007] [PMID]
5. Parolini O, Alviano F, Bagnara GP, Bilic G, Bühring HJ, Evangelista M, et al. Concise review: isolation and characterization of cells from human term placenta: outcome of the first international workshop on placenta derived stem cells. Stem Cells 2008; 26: 300-311. [DOI:10.1634/stemcells.2007-0594] [PMID]
6. He YG, Alizadeh H, Kinoshita K, McCulley JP. Experimental transplantation of cultured human limbal and amniotic epithelial cells onto the corneal surface. Cornea 1999; 18: 570-579. [DOI:10.1097/00003226-199909000-00010]
7. Banas RA, Trumpower C, Bentlejewski C, Marshall V, Sing G, Zeevi A. Immunogenicity and immunomodulatory effects of amnion-derived multipotent progenitor cells. Hum Immunol 2008; 69: 321-328. [DOI:10.1016/j.humimm.2008.04.007] [PMID]
8. Hunt JS, Petroff MG, McIntire RH, Ober C. HLA-G and immune tolerance in pregnancy. FASEB J 2005; 19: 681-693. [DOI:10.1096/fj.04-2078rev] [PMID]
9. Liu YH, Vaghjiani V, Tee JY, To K, Cui P, Oh DY, et al. Amniotic epithelial cells from the human placenta potently suppress a mouse model of multiple sclerosis. PLoS One 2012; 7: e35758. [DOI:10.1371/journal.pone.0035758] [PMID] [PMCID]
10. Wolbank S, Peterbauer A, Fahrner M, Hennerbichler S, Van Griensven M, Stadler G, et al. Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue. Tissue Eng 2007; 13: 1173-1183. [DOI:10.1089/ten.2006.0313] [PMID]
11. Roh DH, Seo MS, Choi HS, Park SB, Han HJ, Beitz AJ, et al. Transplantation of human umbilical cord blood or amniotic epithelial stem cells alleviates mechanical allodynia after spinal cord injury in rats. Cell Transplant 2013; 22: 1577-1590. [DOI:10.3727/096368912X659907] [PMID]
12. Manuelpillai U, Tchongue J, Lourensz D, Vaghjiani V, Samuel CS, Liu A, et al. Transplantation of human amnion epithelial cells reduces hepatic fibrosis in immunocompetent CCl(4)-treated mice. Cell Transplant 2010; 19: 1157-1168. [DOI:10.3727/096368910X504496] [PMID]
13. Murphy S, Lim R, Dickinson H, Acharya R, Rosli S, Jenkin G, et al. Human amnion epithelial cells prevent bleomycin-induced lung injury and preserve lung function. Cell Transplant 2011; 20: 909-923. [DOI:10.3727/096368910X543385] [PMID]
14. Steinman RM, Banchereau J. Taking dendritic cells into medicine. Nature 2007; 449: 419-426. [DOI:10.1038/nature06175] [PMID]
15. Lutz MB, Schuler G. Immature, semi-mature and fully mature dendritic cells: which signals induce tolerance or immunity? Trends Immunol 2002; 23: 445-449. [DOI:10.1016/S1471-4906(02)02281-0]
16. Magatti M, De Munari S, Vertua E, Nassauto C, Albertini A, Wengler GS, et al. Amniotic mesenchymal tissue cells inhibit dendritic cell differentiation of peripheral blood and amnion resident monocytes. Cell Transplant 2009; 18: 899-914. [DOI:10.3727/096368909X471314] [PMID]
17. Magatti M, Caruso M, De Munari S, Vertua E, De D, Manuelpillai U, et al. Human amniotic membrane-derived mesenchymal and epithelial cells exert different effects on monocyte-derived dendritic cell differentiation and function. Cell Transplant 2015; 24: 1733-1752. [DOI:10.3727/096368914X684033] [PMID]
18. Jiang XX, Zhang Y, Liu B, Zhang SX, Wu Y, Yu XD, et al. Human mesenchymal stem cells inhibit differentiation and function of monocyte-derived dendritic cells. Blood 2005; 105: 4120-4126. [DOI:10.1182/blood-2004-02-0586] [PMID]
19. Nauta AJ, Kruisselbrink AB, Lurvink E, Willemze R, Fibbe WE. Mesenchymal stem cells inhibit generation and function of both CD34+-derived and monocyte-derived dendritic cells. J Immunol 2006; 177: 2080-2087. [DOI:10.4049/jimmunol.177.4.2080] [PMID]
20. Ennis J, Gotherstrom C, Le Blanc K, Davies JE. In vitro immunologic properties of human umbilical cord perivascular cells. Cytotherapy 2008; 10: 174-181. [DOI:10.1080/14653240801891667] [PMID]
21. Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol 2005; 129: 118-129. [DOI:10.1111/j.1365-2141.2005.05409.x] [PMID]
22. Tabatabaei M, Mosaffa N, Nikoo S, Bozorgmehr M, Ghods R, Kazemnejad S, et al. Isolation and partial characterization of human amniotic epithelial cells: the effect of trypsin. Avicenna J Med Biotechnol 2014; 6: 10-20.
23. Magatti M, De Munari S, Vertua E, Gibelli L, Wengler GS, Parolini O. Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities. Stem Cells 2008; 26: 182-192. [DOI:10.1634/stemcells.2007-0491] [PMID]
24. Díaz‐Prado S, Muiños‐López E, Hermida‐Gómez T, Rendal‐Vázquez ME, Fuentes‐Boquete I, de Toro FJ, et al. Multilineage differentiation potential of cells isolated from the human amniotic membrane. J Cell Biochem 2010; 111: 846-857. [DOI:10.1002/jcb.22769] [PMID]
25. Pratama G, Vaghjiani V, Tee JY, Liu YH, Chan J, Tan C, et al. Changes in culture expanded human amniotic epithelial cells: implications for potential therapeutic applications. PLoS One 2011; 6: e26136. [DOI:10.1371/journal.pone.0026136] [PMID] [PMCID]
26. Bilic G, Zeisberger SM, Mallik AS, Zimmermann R, Zisch AH. Comparative characterization of cultured human term amnion epithelial and mesenchymal stromal cells for application in cell therapy. Cell Transplant 2008; 17: 955-968. [DOI:10.3727/096368908786576507] [PMID]
27. Cools N, Ponsaerts P, Van Tendeloo VF, Berneman ZN. Balancing between immunity and tolerance: an interplay between dendritic cells, regulatory T cells, and effector T cells. J Leukoc Biol 2007; 82: 1365-1374. [DOI:10.1189/jlb.0307166] [PMID]
28. Chang CC, Wright A, Punnonen J. Monocyte-derived CD1a+ and CD1a− dendritic cell subsets differ in their cytokine production profiles, susceptibilities to transfection, and capacities to direct Th cell differentiation. J Immunol 2000; 165: 3584-3591. [DOI:10.4049/jimmunol.165.7.3584] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
