International Journal of
Reproductive Biomedicine
Thu, Apr 25, 2024
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Volume 18, Issue 3 (March 2020)
IJRM 2020, 18(3): 215-226 |
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Kadir R E, Kadir R E, Ibrahim B A, Musa Gwadabe S, Jaji-Sulaimon R, Adigun M F et al . Low dose bitter leaf improves sperm quality disrupted in immunosuppressed Wistar rats: An experimental study. IJRM 2020; 18 (3) :215-226
URL: http://ijrm.ir/article-1-1088-en.html
URL: http://ijrm.ir/article-1-1088-en.html
Risikat Eniola Kadir1 
, Risikat Eniola Kadir * 2, Balkis Abimbola Ibrahim1 , Sadiya Musa Gwadabe1 , Rukayat Jaji-Sulaimon1 , Munirat Foyeke Adigun1 , Adeoye Oyetunji Oyewopo1
, Risikat Eniola Kadir * 2, Balkis Abimbola Ibrahim1 , Sadiya Musa Gwadabe1 , Rukayat Jaji-Sulaimon1 , Munirat Foyeke Adigun1 , Adeoye Oyetunji Oyewopo1
1- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria.
2- Department of Human Biology, University of Cape Town, Cape Town, South Africa. , ibrahim.am@unilorin.edu.ng
2- Department of Human Biology, University of Cape Town, Cape Town, South Africa. , ibrahim.am@unilorin.edu.ng
Abstract: (2107 Views)
Background: Synthetic prednisolone (PRED) is a widely used over-the-counter glucocorticoid. Glucocorticoids have inhibitory effects on the immune system and are often used as immunosuppressive agents. Suppressed immunity may impact fertility via the hypothalamic-pituitary-adrenal axis. Bitter leaf (BL) has been shown to improve sperm parameters, but its effects on immunosuppression-associated infertility have not yet been documented.
Objective: To determine the fertility effects of bitter leaf on immunosuppressed Wistar rats.
Materials and Methods: A total of 30 male adult Wistar rats were randomly assigned to 6 groups(n= 5/each). Group A served as a control and were given distilled water in addition to normal feeds, group B received 2 mg/kg PRED for 14 days and served as the standard immunosuppressed group, and groups C-F were immunosuppressed as in B but in addition received 50 mg/kg levamisole, low-dose (250 mg/kg) BL, high-dose (375 mg/kg) BL, and low-dose BL + levamisole, respectively. The CD4 counts, hematological parameters, and sperm parameters were analyzed and compared.
Results: There were significant decreases in sperm motility, progressive motility, morphology, and life/death ratio in the animals given PRED only compared to the controls (p = 0.002, 0.001, 0.001, and 0.01, respectively). These were significantly increased in the treated groups, and animals given levamisole and 250 mg/kg BL showed significantly increased sperm counts compared to the controls (p = 0.04 and p = 0.04, respectively).
Conclusion: Low-dose BL (250 mg/kg) restored the sperm parameters altered by prednisolone administration.
Objective: To determine the fertility effects of bitter leaf on immunosuppressed Wistar rats.
Materials and Methods: A total of 30 male adult Wistar rats were randomly assigned to 6 groups(n= 5/each). Group A served as a control and were given distilled water in addition to normal feeds, group B received 2 mg/kg PRED for 14 days and served as the standard immunosuppressed group, and groups C-F were immunosuppressed as in B but in addition received 50 mg/kg levamisole, low-dose (250 mg/kg) BL, high-dose (375 mg/kg) BL, and low-dose BL + levamisole, respectively. The CD4 counts, hematological parameters, and sperm parameters were analyzed and compared.
Results: There were significant decreases in sperm motility, progressive motility, morphology, and life/death ratio in the animals given PRED only compared to the controls (p = 0.002, 0.001, 0.001, and 0.01, respectively). These were significantly increased in the treated groups, and animals given levamisole and 250 mg/kg BL showed significantly increased sperm counts compared to the controls (p = 0.04 and p = 0.04, respectively).
Conclusion: Low-dose BL (250 mg/kg) restored the sperm parameters altered by prednisolone administration.
Type of Study: Original Article |
Subject:
Reproductive Biology
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