Volume 18, Issue 3 (March 2020)                   IJRM 2020, 18(3): 215-226 | Back to browse issues page


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Kadir R E, Kadir R E, Ibrahim B A, Musa Gwadabe S, Jaji-Sulaimon R, Adigun M F et al . Low dose bitter leaf improves sperm quality disrupted in immunosuppressed Wistar rats: An experimental study. IJRM 2020; 18 (3) :215-226
URL: http://ijrm.ir/article-1-1088-en.html
1- Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, PMB 1515, Nigeria.
2- Department of Human Biology, University of Cape Town, Cape Town, South Africa. , ibrahim.am@unilorin.edu.ng
Abstract:   (3240 Views)
Background: Synthetic prednisolone (PRED) is a widely used over-the-counter glucocorticoid. Glucocorticoids have inhibitory effects on the immune system and are often used as immunosuppressive agents. Suppressed immunity may impact fertility via the hypothalamic-pituitary-adrenal axis. Bitter leaf (BL) has been shown to improve sperm parameters, but its effects on immunosuppression-associated infertility have not yet been documented.
Objective: To determine the fertility effects of bitter leaf on immunosuppressed Wistar rats.
Materials and Methods: A total of 30 male adult Wistar rats were randomly assigned to 6 groups(n= 5/each). Group A served as a control and were given distilled water in addition to normal feeds, group B received 2 mg/kg PRED for 14 days and served as the standard immunosuppressed group, and groups C-F were immunosuppressed as in B but in addition received 50 mg/kg levamisole, low-dose (250 mg/kg) BL, high-dose (375 mg/kg) BL, and low-dose BL + levamisole, respectively. The CD4 counts, hematological parameters, and sperm parameters were analyzed and compared.
Results: There were significant decreases in sperm motility, progressive motility, morphology, and life/death ratio in the animals given PRED only compared to the controls (p = 0.002, 0.001, 0.001, and 0.01, respectively). These were significantly increased in the treated groups, and animals given levamisole and 250 mg/kg BL showed significantly increased sperm counts compared to the controls (p = 0.04 and p = 0.04, respectively).
Conclusion: Low-dose BL (250 mg/kg) restored the sperm parameters altered by prednisolone administration.
 
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Type of Study: Original Article | Subject: Reproductive Biology

References
1. Iyalomhe GBS, Iyalomhe SI. Current use of corticosteroids in rheumatology. Int Res J Pharm Pharmacol 2013; 3: 85-90.
2. Shaikh S, Verma H, Yadav N, Jauhari M, Bullangowda J. Applications of steroid in clinical practice: A review. ISRN Anesthesiology 2012; 2012: 1-11. [DOI:10.5402/2012/985495]
3. Mayhew D, Ridgway D, Hunter JM. Update on the intraoperative management of adult cadaveric renal transplantation. BJA Education 2016; 16: 53-57. [DOI:10.1093/bjaceaccp/mkv013]
4. Volkmann ER, Rezai S, Tarp S, Woodworth TG, Furst DE. We still don't know how to taper glucocorticoids in rheumatoid arthritis, and we can do better. J Rheumatol 2013; 40: 1646-1649. [DOI:10.3899/jrheum.130019] [PMID]
5. Ojeifo Uadia P, Mathias Emokpae A. Male infertility in Nigeria: A neglected reproductive health issue requiring attention. Journal of Basic and Clinical Reproductive Sciences 2015; 4: 45-53 [DOI:10.4103/2278-960X.161042]
6. Uadia PO, Emokpae AM. Male infertility in Nigeria: A neglected reproductive health issue requiring attention. Journal of Basic and Clinical Reproductive Sciences 2015; 4: 45-53. [DOI:10.4103/2278-960X.161042]
7. Okonofua F, Menakaya U, Onemu SO, Omo-Aghoja LO, Bergstrom S. A case-control study of risk factors for male infertility in Nigeria. Asian J Androl 2005; 7: 351-361. [DOI:10.1111/j.1745-7262.2005.00046.x] [PMID]
8. Chen Y, Zhang Z, Lin Y, Lin H, Li M, Nie P, et al. Long-term impact of immunosuppressants at therapeutic doses on male reproductive system in unilateral nephrectomized rats: A comparative study. Biomed Res Int 2013: 2013: 690382. [DOI:10.1155/2013/690382] [PMID] [PMCID]
9. Leroy C, Rigot JM, Leroy M, Decanter C, Le Mapihan K, Parent AS, et al. Immunosuppressive drugs and fertility. Orphanet J Rare Dis 2015; 10: 136. [DOI:10.1186/s13023-015-0332-8] [PMID] [PMCID]
10. Denue BA, Gashau W, Bello HS, Kida IM, Bakki B, Ajayi B. Relation between some haematological abnormalities, degree of immunosuppression and viral load in treatment-naïve HIV-infected patients. East Mediterr Health J 2013; 19: 362-368. [DOI:10.26719/2013.19.4.362] [PMID]
11. Saalu LC, Akunna GG, Ajayi JO. Modulating role of bitter leaf on spermatogenic and steroidogenesis functions of the rat testis. Am J Biochem Mol Biol 2013; 3: 314-321. [DOI:10.3923/ajbmb.2013.314.321]
12. Egharevba C, Osayemwenre E, Imieje V, Ahomafor J, Akunyuli Ch, Udu-Cosi AA, et al. Significance of bitter leaf (vernonia amagdalina) in tropical diseases and beyond: a review. Malar Chemoth Cont 2014; 3: doi:10.4172/2090-2778.1000120. [DOI:10.4172/2090-2778.1000120]
13. Grubben GJH, Denton OA. Plant Resources of Tropical Africa 2 Vegetables. Backhuys Publishers, Wageningen, Netherlands; 2004: 543.
14. Oyeyemi MO, Oluwatoyin O, Ajala Leigh OO, Adesiji TF. The spermiogram of male wistar rats treated with aqueous leaf extract of Venonia amygdalina. Folia Veterinaria 2007; 2: 126-129.
15. Gray DW, Millard PR, McShane P, Morris PJ. The use of the dye neutral red as a specific, non-toxic, intra-vital stain of islets of Langerhans. Br J Exp Pathol 1983; 64: 553-558.
16. Fischer AH, Jacobson KA, Rose J, Zeller R. Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc 2008; 2008: pdb prot 4986. [DOI:10.1101/pdb.prot4986] [PMID]
17. Saalu LC, Osinubi AA, Akinbami AA, Yama OE, Oyewopo AO, Enaibe BU. Moringa oleifera lamarck (drumstick) leaf extract modulates the evidences of hydroxyurea-induced testicular derangement. Int J Applied Res Nat Prod 2011; 4: 32-45.
18. Gupta M. Levamisole: A multi-faceted drug in dermatology. Indian J Dermatol Venereol Leprol 2016; 82: 230-236. [DOI:10.4103/0378-6323.175927] [PMID]
19. Zhu J, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations. Annu Rev Immunol 2010; 28: 445-489. [DOI:10.1146/annurev-immunol-030409-101212] [PMID] [PMCID]
20. Shahapur PR, Bidri RC. Recent trends in the spectrum of opportunistic infections in human immunodeficiency virus infected individuals on antiretroviral therapy in South India. J Nat Sc Biol Med 2014; 5: 392-396. [DOI:10.4103/0976-9668.136200] [PMID] [PMCID]
21. Weatherspoon D. WBC (White blood cell) count. Available at: https://www.healthline.com/health/wbc-count.
22. Ohkaru Y, Arai N, Ohno H, Sato Sh, Sakakibara Y, Suzuki H, et al. Acute and subacute effects of dexamethasone on the number of white blood cells in rats. Journal of Health Science 2010; 56: 215-220. [DOI:10.1248/jhs.56.215]
23. Amar MI, Adam Shama IY, Enaia AA, Hind AEO, Hager AM. Effects of various levels of oral doses dexamethasone (al-nagma) abused as cosmetic by sudanese women on wistar rats. Journal of Medical Sciences 2013; 13: 432-438. [DOI:10.3923/jms.2013.432.438]
24. Praituan W, Rojnuckarin P. Faster platelet recovery by high-dose dexamethasone compared with standard-dose prednisolone in adult immune thrombocytopenia: a prospective randomized trial. J Thromb Haemost 2009; 7: 1036-1038. [DOI:10.1111/j.1538-7836.2009.03359.x] [PMID]
25. Narimane K, Mustapha K, Nedjoua L. Effects of Anti-Inflammatory and Immunosuppressive Doses of Dexamethasone on Blood Parameters in Ouled Djellal Sheep. Arch Appl Sci Res 2017; 9: 11-15.
26. Thanasak J, Jorritsma R, Hoek A, Noordhuizen JP, Rutten VP, Muller KE. The effects of a single injection of dexamethasone-21-isonicotinate on the lymphocyte functions of dairy cows at two weeks post partum. Vet Res 2004; 35: 103-112. [DOI:10.1051/vetres:2003040] [PMID]
27. Akbari F, Alavi M, Esteghamati A, Mehrsai A, Djaladat H, Zohrevand R, et al. Effect of renal transplantation on sperm quality and sex hormone levels. BJU Int 2003; 92: 281-283. [DOI:10.1046/j.1464-410X.2003.04323.x] [PMID]
28. Seethalakshmi L, Flores C, Carboni AA, Bala R, Diamond DA, Menon M. Cyclosporine: its effects on testicular function and fertility in the prepubertal rat. J Androl 1990; 11: 17-24.
29. Gawish MA, ElFiky S, Therase M, AbdElraaof A, Khalil W, Mohamed KA. Sperm abnormality toxicity due to cyclosporine A and the ameliorative effect of royal jelly in male rats. The Journal of Basic & Applied Zoology 2016; 76: 60-73. [DOI:10.1016/j.jobaz.2016.12.001]
30. Dua A, Vaidya SR. Sperm motility and morphology as changing parameters linked to sperm count variations. J Postgrad Med 1996; 42: 93-96.
31. Igile G, Oleszek W, Jurzysta M, Aquino R, Tommasi Nd, Pizza C. Vernoniosides D and E, two noveal saponins from vernonia amygdalina. J Nat Prod 1995; 58: 1438-1443. [DOI:10.1021/np50123a016]
32. Bozkurt T, Tanyildizi S, Turk G. Effects of levamisole on hyaluronidase activity and sperm characteristics in rams. Theriogenology 2004; 62: 323-329. [DOI:10.1016/j.theriogenology.2003.10.009] [PMID]

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