International Journal of
Reproductive Biomedicine
Tue, Apr 16, 2024
[Archive]
Volume 16, Issue 5 (May 2018)
IJRM 2018, 16(5): 299-304 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Arefi S, Fazeli E, Esfahani M, Borhani N, Yamini N, Hosseini A et al . Granulocyte-colony stimulating factor may improve pregnancy outcome in patients with history of unexplained recurrent implantation failure: An RCT. IJRM 2018; 16 (5) :299-304
URL: http://ijrm.ir/article-1-1105-en.html
URL: http://ijrm.ir/article-1-1105-en.html
Soheila Arefi * 
1, Elham Fazeli2 , Manijeh Esfahani3 , Nasim Borhani4 , Nazila Yamini5 , Ahmad Hosseini3 , Fattaneh Farifteh3
1, Elham Fazeli2 , Manijeh Esfahani3 , Nasim Borhani4 , Nazila Yamini5 , Ahmad Hosseini3 , Fattaneh Farifteh3
1- Genetics and In Vitro Assisted Reproductive (GIVAR) Center, Erfan Hospital, Tehran, Iran , f.farifteh@gmail.com
2- Department of Anatomy and Reproductive Biology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Genetics and In Vitro Assisted Reproductive (GIVAR) Center, Erfan Hospital, Tehran, Iran
4- School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
5- IVF Center, Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran
2- Department of Anatomy and Reproductive Biology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Genetics and In Vitro Assisted Reproductive (GIVAR) Center, Erfan Hospital, Tehran, Iran
4- School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
5- IVF Center, Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran
Abstract: (4273 Views)
Background: Family of colony-stimulating factors (CSF) have an essential role on early cross talk between embryo and uterine endometrium.
Objective: The aim of this study was to evaluate the effects of the single dose of Granulocyte-CSF (G-CSF) injection on clinical outcome of assisted reproductive technology cycle in patients with repeated implantation failures.
Materials and Methods: This randomized control trial study was performed on 52 infertile women who referred to the clinic with the history of more than three previous In vitro fertilization/Intracytoplasmic sperm injection-embryo transfer failures. All patients were stimulated with standard long protocol. All embryos were transferred on day five in blastocyst stage in both groups. The treated group received 300 μg (0.5 ml) recombinant human G-CSF subcutaneously which was injected 30 min before blastocyst embryo transfer.
Results: There was not statistically significant differences in abortion rate in G-CSF and control group (p=0.09). G-CSF treated group showed higher clinical pregnancy rate in comparison with control group (56.2% vs. 40.0%) but it was not statistically significant (p=0.09). Although live birth rate in G-CSF group was higher than control group (53.1% vs. 35.0%) but there wasn’t statistically significant difference in the overall live birth rate between the two groups (p=0.10). G-CSF group had a twin pregnancies while in control group there was no twin pregnancy.
Conclusion: Our result demonstrates the possibility that pregnancy outcome is better in women with repeated unexplained In vitro fertilization failure who are treated with G-CSF.
Objective: The aim of this study was to evaluate the effects of the single dose of Granulocyte-CSF (G-CSF) injection on clinical outcome of assisted reproductive technology cycle in patients with repeated implantation failures.
Materials and Methods: This randomized control trial study was performed on 52 infertile women who referred to the clinic with the history of more than three previous In vitro fertilization/Intracytoplasmic sperm injection-embryo transfer failures. All patients were stimulated with standard long protocol. All embryos were transferred on day five in blastocyst stage in both groups. The treated group received 300 μg (0.5 ml) recombinant human G-CSF subcutaneously which was injected 30 min before blastocyst embryo transfer.
Results: There was not statistically significant differences in abortion rate in G-CSF and control group (p=0.09). G-CSF treated group showed higher clinical pregnancy rate in comparison with control group (56.2% vs. 40.0%) but it was not statistically significant (p=0.09). Although live birth rate in G-CSF group was higher than control group (53.1% vs. 35.0%) but there wasn’t statistically significant difference in the overall live birth rate between the two groups (p=0.10). G-CSF group had a twin pregnancies while in control group there was no twin pregnancy.
Conclusion: Our result demonstrates the possibility that pregnancy outcome is better in women with repeated unexplained In vitro fertilization failure who are treated with G-CSF.
Keywords: Granulocyte colony-stimulating factor, Embryo implantation, Pregnancy rates, Assisted reproductive technology, Randomized controlled trial.
Type of Study: Original Article |
References
1. Simon A, Laufer N. Repeated implantation failure: clinical approach. Fertil Steril 2012; 97: 1039-1043. [DOI:10.1016/j.fertnstert.2012.03.010]
2. Zeyneloglu HB, Onalan G, Durak T, Alyazici I, Unal E. Granulocyte macrophage colony stimulating factor (G-CSF) administration for art patients with repeated implantation failure (RIF): which route is best? Fertil Steril 2013; 100: S291-S292. [DOI:10.1016/j.fertnstert.2013.07.1031]
3. Lédée N, Gridelet V, Ravet S, Jouan C, Gaspard O, Wenders F, et al. Impact of follicular G-CSF quantification on subsequent embryo transfer decisions: a proof of concept study. Hum Reprod 2013; 28: 406-413. [DOI:10.1093/humrep/des354]
4. Cavalcante MB, Costa Fda S, Barini R, Araujo Júnior E. Granulocyte colony-stimulating factor and reproductive medicine: A review. Iran J Reprod Med 2015; 13: 195-202.
5. Kahyaoglu I, Yılmaz N, Timur H, Inal HA, Erkaya S. Granulocyte colony-stimulating factor: A relation between serum and follicular fluid levels and in-vitro fertilization outcome in patients with polycystic ovary syndrome. Cytokine 2015; 74: 113-116. [DOI:10.1016/j.cyto.2014.09.002]
6. Würfel W. Treatment with granulocyte colony-stimulating factor in patients with repetitive implantation failures and/or recurrent spontaneous abortions. J Reprod Immunol 2015; 108: 123-135. [DOI:10.1016/j.jri.2015.01.010]
7. Meier P, Gloekler S, Oezdemir B, Indermuehle A, Traupe T, Vogel R, et al. G-CSF induced arteriogenesis in humans: molecular insights into a randomized controlled trial. Cur Vasc Pharmacol 2013; 11: 38-46. [DOI:10.2174/157016113804547674]
8. Sugita K, Hayakawa S, Karasaki‐Suzuki M, Hagiwara H, Chishima F, Aleemuzaman S, et al. Granulocyte colony stimulation factor (G‐CSF) suppresses interleukin (IL)‐12 and/or IL‐2 induced interferon (IFN)‐γ production and cytotoxicity of decidual mononuclear cells. Am J Reprod Immunol 2003; 50: 83-89. [DOI:10.1034/j.1600-0897.2003.00024.x]
9. Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N. Colony stimulating factors 1, 2, 3 and early pregnancy steps: from bench to bedside. J Reprod Immunol 2015; 109: 1-6. [DOI:10.1016/j.jri.2015.01.005]
10. Frydman R, Osipova A, Piccini M-P, Petitbarat M, Frydman N, Ledee N. The predictive role of granulocyte colony-stimulating factor and others cytokines in follicular fluid on the embryo implantation. Fertil Steril 2009; 92: S249. [DOI:10.1016/j.fertnstert.2009.07.1631]
11. Lédée N, Petitbarat M, Rahmati M, Dubanchet S, Chaouat G, Sandra O, et al. New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side. J Reprod Immunol 2011; 88: 118-123. [DOI:10.1016/j.jri.2011.01.007]
12. Lédée N, Frydman R, Osipova A, Taieb J, Gallot V, Lombardelli L, et al. Levels of follicular G-CSF and interleukin-15 appear as noninvasive biomarkers of subsequent successful birth in modified natural in vitro fertilization/intracytoplasmic sperm injection cycles. Fertil Steril 2011; 95: 94-98. [DOI:10.1016/j.fertnstert.2010.03.010]
13. Kamath MS, Chittawar PB, Kirubakaran R, Mascarenhas M. Use of Granulocyte-colony stimulating factor in assisted reproductive technology: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol 2017; 214: 16-24. [DOI:10.1016/j.ejogrb.2017.04.022]
14. Asl ZA. The efficacy of systemic administration of granulocyte colony stimulating factor (GCSF) on the in vitro fertilization (IVF) success in women with repeated implantation failure. Fertil Steril 2015; 104: e61. [DOI:10.1016/j.fertnstert.2015.07.185]
15. Salmassi A, Schmutzler AG, Schaefer S, Koch K, Hedderich J, Jonat W, et al. Is granulocyte colony-stimulating factor level predictive for human IVF outcome? Hum Reprod 2005; 20: 2434-2440. [DOI:10.1093/humrep/dei071]
16. Gellersen B, Brosens IA, Brosens JJ: Decidualization of the human endometrium: mechanisms, functions, and clinical perspectives. Semin Reprod Med 2007; 25: 445-453. [DOI:10.1055/s-2007-991042]
17. Eftekhar M, Miraj S, Farid Mojtahedi M, Neghab N. Efficacy of intrauterine infusion of granulocyte colony stimulating factor on patients with history of implantation failure: A randomized control trial. Int J Reprod Biomed 2016; 14: 687-690. [DOI:10.29252/ijrm.14.11.687]
18. Würfel W, Santjohanser C, Hirv K, Bühl M, Meri O, Laubert I, et al. High pregnancy rates with administration of granulocyte colony-stimulating factor in ART-patients with repetitive implantation failure and lacking killer-cell immunglobulin-like receptors. Hum Reprod 2010; 25: 2151-2152. [DOI:10.1093/humrep/deq106]
19. Scarpellini F, Sbracia M. Use of granulocyte colony-stimulating factor for the treatment of unexplained recurrent miscarriage: a randomised controlled trial. Hum Reprod 2009; 24: 2703-2708. [DOI:10.1093/humrep/dep240]
20. Santjohanser C, Knieper C, Franz C, Hirv K, Meri O, Schleyer M, et al. Granulocyte-colony stimulating factor as treatment option in patients with recurrent miscarriage. Arch Immunol Ther Exp 2013; 61: 159-164. [DOI:10.1007/s00005-012-0212-z]
21. Gleicher N, Kim A, Michaeli T, Lee H, Shohat-Tal A, Lazzaroni E, et al. A pilot cohort study of granulocyte colony-stimulating factor in the treatment of unresponsive thin endometrium resistant to standard therapies. Hum Reprod 2013; 28: 172-177. [DOI:10.1093/humrep/des370]
22. Lieschke G, Grail D, Hodgson G, Metcalf D, Stanley E, Cheers C, et al. Mice lacking granulocyte colony-stimulating factor have chronic neutropenia, granulocyte and macrophage progenitor cell deficiency, and impaired neutrophil mobilization. Blood 1994; 84: 1737-1746.
23. Seymour JF, Lieschke GJ, Grail D, Quilici C, Hodgson G, Dunn AR. Mice lacking both granulocyte colony-stimulating factor (CSF) and granulocyte-macrophage CSF have impaired reproductive capacity, perturbed neonatal granulopoiesis, lung disease, amyloidosis, and reduced long-term survival. Blood 1997; 90: 3037-3049.
24. Litwin S, Lagadari M, Barrientos G, Roux ME, Margni R, Miranda S. Comparative Immunohistochemical Study of M‐CSF and G‐CSF in Feto–Maternal Interface in a Multiparity Mouse Model. Am J Reprod Immunol 2005; 54: 311-320. [DOI:10.1111/j.1600-0897.2005.00317.x]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
