Volume 6, Issue 4 (7-2008)                   IJRM 2008, 6(4): 143-0 | Back to browse issues page

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Absalan F, Movahedin M, Mowla S J. Assessment of testis histological changes and sperm parameters in experimentally–induced unilateral and bilateral cryptorchid mouse model. IJRM 2008; 6 (4) :143-0
URL: http://ijrm.ir/article-1-114-en.html
Assessment of testis histological changes and sperm parameters in experimentally–induced unilateral and bilateral cryptorchid mouse model
Forouzan Absalan1 , Mansoureh Movahedin * 2, Seyed Javad Mowla3
1- Department of Anatomical Sciences, School of Medical Sciences, Tehran, Iran
2- Department of Anatomical Sciences, School of Medical Sciences, Tehran, Iran , mansoure@modares.ac.ir
3- Department of Genetics, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (2893 Views)
Background: In most mammals, the testis is always maintained at a lower temperature than that in the abdomen, and exposure of the testis to body temperature causes degeneration of germ cells.
Objective: In this research, the long effect of heat exposure on sperm parameters and microstructure of mouse testis were investigated. Cryptorchid mouse were induced by exposure to abdominal heat.
Materials and Methods: Immature mice were anesthetized and a small incision was made in the abdominal skin, then fat pad at the upper end of testis was sutured to peritoneum. Weight of testis, spermatogenic cell numbers, tubular ectasis (rate of tubular lumen comparing to the thickness of germinal epithelium) as well as epididymal sperm parameters were measured.
Results: The results showed that spermatogenesis was arrested and testicular weights, seminiferouse tubular diameters and epididymal sperm parameters were significantly reduced in the bilateral undescended testis compared with unilateral undescended testis and the control mice. However, complete depletion of seminiferous tubules and absence of germ cells was not found in the animals.
Conclusion: In general, high temperature caused a decreased in all analyzed parameters except spermatogonial cell number probably due to the apoptosis and these changes significantly increase in bilateral groups compared with unilateral groups. We believe that the present model is a suitable tool for enrichment of spermatogonial stem cells, also it is useful for treatment of cryptorchidism and further biological research on spermatogenesis.
Keywords: Cryptorchidism, Testis, Spermatogonial stem cells
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Type of Study: Original Article |
References
1. Rockett JC, Map FL, Gares JB. Effects of hyperthermia on spermatogenesis, apoptosis, gene expression and fertility in adult male mice. Biol Reprod 2001; 65: 229-239 [DOI:10.1095/biolreprod65.1.229]
2. Jackson MB. The epidemiology of cryptorchidism. John Radcliffe Hospital Cryptorchidism Research Group. Horm Res 1998; 30: 153-6. [DOI:10.1159/000181052]
3. Ito K, Tanemura K, Gotch H, Kurohmura M, Hayashi Y. Apoptosis-like cell death in experimentally- induced cryptorchidism in adult mice. J Vet Med Sci 1997; 59: 353-359. [DOI:10.1292/jvms.59.353]
4. Kerr JB, Rich KA, de Krester DM. Effects of experimental cryptorchidism on the ultrastructure and function of the sertoli cell and peritubular tissue of the rat testis. Biol Reprod 1979; 21: 823-838. [DOI:10.1095/biolreprod21.4.823]
5. Damber JE, Bergh A, Janson PO. Testicular blood flow and testosterone concentrations in the spermatic venous blood in rats with experimental cryptorchidism. Acta Endocrinol 1978; 88: 611-618. [DOI:10.1530/acta.0.0880611]
6. Kerr JB, Rich KA, de Krester DM. Alteration of the fine structure and androgen secretion of the interstitial cells in the experimentally cryptorchid rat testis. Biol Reprod 1979; 20: 409-422. [DOI:10.1095/biolreprod20.3.409]
7. Hall PF, Kew D, Mita M. The influence of temperature on the function of cultured sertoli cells. Endocrinology 1985; 116: 1926-1932. [DOI:10.1210/endo-116-5-1926]
8. Wang ZQ, Todani T, Watanabe Y, Toki A, Ogura K, Miamoto O, et al. Germ cell degeneration in experimental unilateral cryptorchidism; role of apoptosis. Pediatr Surg Int 1998; 14: 9-13. [DOI:10.1007/s003830050424]
9. Blackshaw AW, Massey PF. Early histological and histochemical changes in the heated and cryptorchid rat testis. Aust J Biol Sci 1978; 31: 53-64.
10. Mieusset R, Bujan L. Testicular heating and its possible contributions to male infertility: a review. Int J Androl 1995; 18: 169-184. [DOI:10.1111/j.1365-2605.1995.tb00408.x]
11. de Rooij DJ, Grootegoud JA. Spermatogonial stem cells. Curr Opin Cell Biol 1998; 10:694-701. [DOI:10.1016/S0955-0674(98)80109-9]
12. Nishimune Y, Aizawa S, Kumatso T. Testicular germ cell differentiation in vivo. Fertil Steril 1978; 29: 95-102. [DOI:10.1016/S0015-0282(16)43045-1]
13. Taki TM, Nickerson PA. Validation of cell volume determination for stereological studies of adrenocortical cells: comparison of values from semifine sections with those of dissociated zona faciculata cells. Am J Anat 1984; 171: 415- 426. [DOI:10.1002/aja.1001710406]
14. World Health Organization 1999 WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge, UK: Cambridge University Press.
15. Nelson WO. Mammalian spermatogenesis effects of experimental cryptorchidism in the rat and non-descend of the testis in man. Rec Prog Horm Res 1995; 6: 29-62.
16. Yavetz H, Harash B, Paz G, Yogev A, Jaffa A, Lessing JB. Cryptorchidism: incidence and sperm quality in infertile men. Andrologia 1992; 24: 293-297. [DOI:10.1111/j.1439-0272.1992.tb02655.x]
17. Bartlett JMS, Sharp RM. Effect of local heating of the rat testis on the levels in interstitial fluid of a putative paracrine regulator of the leydig cells and its relationship to changes in sertoli cell secretory function. J Reprod Fertil 1987; 80:279-287. [DOI:10.1530/jrf.0.0800279]
18. Chowdhury AK, Stainberger E. A quantitative study of the effect of heat on the germinal epithelium of rat testes. American J Anatomy1964; 115: 509-524. [DOI:10.1002/aja.1001150307]
19. Chowdhury AK, Stainberger E. Early changes in the germinal epithelium of rat testes following exposure to heat. J Reprod Fertil 1970; 22: 205-212. [DOI:10.1530/jrf.0.0220205]
20. Collis PM, Lacy D. Studies on the structure and function of the mammalian testes. Cytological and histochemical observations pn the testis of the rat after a single exposure to heat applied for different lengths of time. Proceedings of the Royal Society of London B 1969; 172: 17-38. [DOI:10.1098/rspb.1969.0009]
21. Thoshihiko S, Hakan B, Aaron JW. Experimentally induced cryptorchidism increases apoptosis in rat testis. Biol Reprod 1994; 51: 865-872. [DOI:10.1095/biolreprod51.5.865]
22. Longquan R, Mohamed S, Marik O. Effects of experimental cryptorchidism on sperm motility and testicular endocrinology in adult male rats. J of Report Develop 2006; 52: 219-228. [DOI:10.1262/jrd.17073]
23. Patkowski JR, Czernik J, Jelen M. The natural course of cryptorchidism in rats and the efficacy of orchidopexy or orchidectomy in its treatment before and after puberty. J Pediater Surg 1992; 27:870-879. [DOI:10.1016/0022-3468(92)90387-M]
24. Shinohara T, Mary R, Avarbock, Brinster RF. Functional analysis of spermatogonial stem cells in steel and cryotorchid infertile mouse model. Dev Biol 2000; 220: 401-411. [DOI:10.1006/dbio.2000.9655]
25. Ogawa t, Ohmura M, Yumura y, Savada H, Honji T, Kubuta Y. Expansion of murine spermatogonial stem cell throught serial transplantation. Biol Reprod 2003; 68; 316-322. [DOI:10.1095/biolreprod.102.004549]
26. Anjamrooz SH, Movahedin M, Mowla SJ, Pour Bairanvand S. Assessment of morphological and functional changes in the mouse testis and epididymal sperms following busulfan treatment. Iran Biomed J 2006; 11; 15-22.
27. Shinohara T, Orwig KE, Avarbock MR, Brinster RL. Remodeling of the post natal mouse testis in accompanied by dramatic changes in stem cell number and niche accessibility. Proc Natl Acad Sci USA 2001; 98: 6186-6191. [DOI:10.1073/pnas.111158198]
28. Ried BO, Mason KA, Withers HR, West J. Effects of hyperthermia and radiation on mouse testis stem cells. Cancer Research 1981; 41: 4453-4457.
29. de Rooij DJ, Okabe M, Nishimune Y. Arrest of spermatogonial differentiation in jsd/jsd, S117H/S117H and cryptorchid mice. Biol Reprod 1999; 61: 842-84. [DOI:10.1095/biolreprod61.3.842]
30. Hochereau-de Reviers MT, Locatelli A, Perreau C, Pisselet C, Setchell BP. Effects of a single brief period of moderate heating of the testes on seminiferous tubules in hypophysectomized rams treated with pituatory extract. J Reprod Fertil 1993; 97: 381-387. [DOI:10.1530/jrf.0.0970381]
31. Miralgia SM, Hayashi H. Histomorphometry of immature rat testis after heating. J Morpho 1993; 217: 65-74. [DOI:10.1002/jmor.1052170106]
32. Shikone T, Billing H, Hsueh AJW. Experimentally induced cryptorchidism increases apoptosis in rat testis. Biol of Reprod 1994; 51: 895-872. [DOI:10.1095/biolreprod51.5.865]
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