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Jorsaraei S G A, Shibahara H, Hirano Y, Shiraishi Y, khalatbari A, Yousofnia Pasha Y et al . The in-vitro effects of nicotine, cotinine and leptin on sperm parameters analyzed by CASA system. IJRM 2008; 6 (4) :157-0
URL: http://ijrm.ir/article-1-116-fa.html
URL: http://ijrm.ir/article-1-116-fa.html
The in-vitro effects of nicotine, cotinine and leptin on sperm parameters analyzed by CASA system. International Journal of Reproductive BioMedicine. 1387; 6 (4) :157-0
چکیده: (2588 مشاهده)
Backgrand: Cotinine (COT) is a major degradation product of nicotine (NIC). The participation of leptin in female reproduction is well-established, but any role in male reproductive function is at the best tenuous.
Objective: The aim of this study was to evaluate the in-vitro effects of nicotine, cotinine and leptin on sperm parameters in normal semen of non-smokers fertile men.
Materials and Methods: Ten healthy nonsmokers aged 25-40 years old were devided into 7 groups, Thier semens were divided into 7 aliquots. (A) was layered with basal solution 70 ng/ml NIC, (B) 35 µg/ml NIC, (C) 300 ng/ml COT, (D) 200 µg/ml COT, (E) 30 ng/ml leptin, (F) 300 ng/ml leptin respectively and (G) was layered with mHTF. After migration, the samples were examined at time 0, +1, +2, +4, +8, and + 24 h of incubation.
Results: These findings were obtained: sperm count: 75.66±66.25x10 6 /ml, forward motility: 75.55±14.80%, progress: 33.66±13.01, VSL: 51.58±6.99 µm/s, VCL: 103.33±14.52 µm/s, ALH: 4.33±0.77 µm, BCF: 25.60±2.97 HZ, STR: 79.33±8.04 %, LIN: 52.55±10.52 %, ELO: 74.22 ± 12.76 % and ARE: 3.04 ± 1.50 u/sq. The parameters were similar before 8 hr and were being decreased after that.
Conclusion: According to the results. nicotin and cotinin have negative effects on the sperm parameters but despite the positive effect of leptin, there is no correlation between leptin concentration in semen and its physical characteristics.
Objective: The aim of this study was to evaluate the in-vitro effects of nicotine, cotinine and leptin on sperm parameters in normal semen of non-smokers fertile men.
Materials and Methods: Ten healthy nonsmokers aged 25-40 years old were devided into 7 groups, Thier semens were divided into 7 aliquots. (A) was layered with basal solution 70 ng/ml NIC, (B) 35 µg/ml NIC, (C) 300 ng/ml COT, (D) 200 µg/ml COT, (E) 30 ng/ml leptin, (F) 300 ng/ml leptin respectively and (G) was layered with mHTF. After migration, the samples were examined at time 0, +1, +2, +4, +8, and + 24 h of incubation.
Results: These findings were obtained: sperm count: 75.66±66.25x10 6 /ml, forward motility: 75.55±14.80%, progress: 33.66±13.01, VSL: 51.58±6.99 µm/s, VCL: 103.33±14.52 µm/s, ALH: 4.33±0.77 µm, BCF: 25.60±2.97 HZ, STR: 79.33±8.04 %, LIN: 52.55±10.52 %, ELO: 74.22 ± 12.76 % and ARE: 3.04 ± 1.50 u/sq. The parameters were similar before 8 hr and were being decreased after that.
Conclusion: According to the results. nicotin and cotinin have negative effects on the sperm parameters but despite the positive effect of leptin, there is no correlation between leptin concentration in semen and its physical characteristics.
نوع مطالعه: Original Article |
فهرست منابع
1. Pasqualotto FF, Sobreiro BP, Hallak J, Pasqualotto EB, Lucon AM. Cigarette smoking is related to a decrease in semen volume in a population of fertile men. BJU Int 2006; 97: 324-326. [DOI:10.1111/j.1464-410X.2005.05906.x]
2. Zhang JP, Meng QY, Zhang LJ, Mao YL, Sun ZX. Effect of smoking on semen quality of infertile men in Shandong, china. Asian J Androl 2000; 2: 143-146.
3. Rajpurkar A, Jiang Y, Dhabuwala CB, Dunbar JC, Li H. Cigarette smoking induces apoptosis in rat testis. J Environ Pathol Toxicol Onco 2002; 21: 243-248. [DOI:10.1615/JEnvironPatholToxicolOncol.v21.i3.50]
4. Evans HJ, Fletcher J, Torrance M, Hargreave TB. sperm abnormalities and cigarette smoking. Lancet 1981; 1: 627-629. [DOI:10.1016/S0140-6736(81)91550-6]
5. Gaur DS, Talekar M, Pathak VP. Effect of cigarette smoking on semen quality of infertile men. Singapore Med J 2007; 48: 119-123.
6. Ramlau-Hansen CH, Thulstrup AM, Aggerholm AS, Jensen MS, Toft G, Bonde JP. Is smoking a risk factor for decreased semen quality? A cross-sectional analysis. Hum Reprod 2007; 22: 188-196. [DOI:10.1093/humrep/del364]
7. Sepaniak S, Forges T, Gerard H, Foliguet B, Bene MC, Monnier-Barbarino P. The influence of cigarette smoking on human sperm quality and DNA fragmentation. Toxicology. 2006; 223: 54-60. [DOI:10.1016/j.tox.2006.03.001]
8. Pankow JF. A consideration of the role of gas/particle partitioning in the deposition of nicotine and other tobacco smoke compounds in the respiratory tract. Chem Res Toxicol 2001; 14: 1465-1481. [DOI:10.1021/tx0100901]
9. Gocze PM, Szabo I, Freeman DA. Influence of nicotine, cotinine, anabasine and cigarette smoke extract on human granulosa cell progesterone and estradiol synthesis Gynecol. Endocrinol 1999; 13: 266-272. [DOI:10.3109/09513599909167565]
10. Lerman C, Tyndale R, Patterson F, Wileyto EP, Shields PG, Pinto A, et al. Nicotine metabolite ratio predicts efficacy of transdermal nicotine for smoking cessation. Clin Pharmacol Ther 2006; 79: 600-608. [DOI:10.1016/j.clpt.2006.02.006]
11. Binnie V, McHugh S, Macpherson L, Borland B, Moir K, Malik K. The validation of self-reported smoking status by analysing cotinine levels in stimulated and unstimulated saliva, serum and urine.Oral Dis 2004; 10: 287-293. [DOI:10.1111/j.1601-0825.2004.01018.x]
12. Kapawa A, Giannakis D, Tsoukanelis K, Kanakas N, Baltogiannis D, Agapitos E, et al. Sofikitis NEffects of paternal cigarette smoking on testicular function, sperm fertilizing capacity, embryonic development, and blastocyst capacity for implantation in rats. Andrologia 2004; 36: 57-68. [DOI:10.1111/j.1439-0272.2004.00605.x]
13. Reddy A, Sood A, Rust PF, Busby JE, Varn E, Mathur RS, et al. The effect of nicotine on in-vitro sperm motion characteristics. J Assist Repord Genet 1995; 12: 217-223. [DOI:10.1007/BF02211802]
14. Aydos K, Guven Mc, Can B, Ergun A. Nicotine toxicity to the ultrastructure of the testis in rats. BJU Int 2001; 88: 622-626. [DOI:10.1046/j.1464-4096.2001.02384.x]
15. Riesenfeld A, oliva H. Effects of nicotine on the fertility, cytology and life span of male rats. Acta Anat (Basel) 1988; 131: 171-176. [DOI:10.1159/000146508]
16. Fávaro WJ, Cagnon VH. Morphometric, Morphological features of the ventral prostate in rats submitted to chronic nicotine and alcohol treatment.Tissue Cell 2006; 38: 311-323. [DOI:10.1016/j.tice.2006.08.002]
17. Wong WY, Thomas CM, Merkus HM, Zielhuis GA, Doesburg WH, Steegers-Theunissen RP. Cigarette smoking and the risk of male factor subfertility: Minor association between cotinine in seminal plasma and semen morphology. Fertil Ssteril 2000; 74: 930-935. [DOI:10.1016/S0015-0282(00)01567-3]
18. Glander HJ, Lammert A, Paasch U, Glasow A, Kratzsch J. Leptin exists in tubuli seminiferi and in seminal plasma. Andrologia 2002; 34: 227-233. [DOI:10.1046/j.1439-0272.2002.00501.x]
19. Smith GD, Jackson LM, Foster DL. Leptin regulation of reproductive function and fertility. Theriogenology 2002; 57: 73-86. [DOI:10.1016/S0093-691X(01)00658-6]
20. Camina JP, Lage M, Menendez C, Grana M, Garcia-Devesa J, Dieguez C, et al. Evidence of free leptin in human seminal plasma. Endocrine 2002 ; 17: 169-174. [DOI:10.1385/ENDO:17:3:169]
21. Kiess W, Müller G, Galler A, Reich A, Deutscher J, Klammt J, et al. Body fat mass, leptin and puberty.J Pediatr Endocrinol Metab 2000; 13 Suppl 1: 717-722. [DOI:10.1515/JPEM.2000.13.S1.717]
22. Branian JD, Hansen kA. Leptin and ovarian folliculogenesis: Implications for ovulation induction and ART outcomes. Semin Reprod Med 2002; 20: 103-112. [DOI:10.1055/s-2002-32501]
23. Tena-sempere M, Barreiro ML. Leptin in male reproduction: The testis paradigm. Mol/9-13 cell Endocrinol 2002; 188: 9-3. [DOI:10.1016/S0303-7207(02)00008-4]
24. Moschos S, Chan JL, Mantzoros CS. Leptin and reproduction: A review. Fertil Steril 2002; 77: 433-444. [DOI:10.1016/S0015-0282(01)03010-2]
25. Salvador J, Gomez -Ambrosi J, Fruhbeck G. Perspectives in the therapeutic use of leptin. Expert opin pharmaco ther 2001; 2: 1615-1622. [DOI:10.1517/14656566.2.10.1615]
26. Gandini L, Lombardo F, Lenzi A, Culasso F, Pacifici R, Zuccaro P, et al. The in-vitro effects of nicotine and cotinine on sperm motility.Hum Reprod 1997;12: 727-233. [DOI:10.1093/humrep/12.4.727]
27. Guo X, Chen S, Xing F. Effects of leptin on estradiol and progesterone production by human luteinized granulosa cells in-vitro. Zhonghua Fu Chan Ke Za Zhi 2001;36:95-97.
28. Jin L, Zhang S, Burguera BG, Couce ME, Osamura RY, Kulig E, et al. Leptin and leptin receptor expression in rat and mouse pituitary cells. Endocrinology 2000; 141:333-339. [DOI:10.1210/endo.141.1.7260]
29. Karlsson C, Lindell K, Svensson E, Bergh C, Lind P, Billig H, et al. Expression of functional leptin receptors in the human ovary. Clin Endocrinol Metab 1997; 82: 4144-4148. [DOI:10.1210/jc.82.12.4144]
30. Kitawaki J, Koshiba H, Ishihara H, Kusuki I, Tsukamoto K, Honjo H. Expression of leptin respectors in human endometrium and fluctuation during the menstrual Cycle. J Clin Endocrinol Metab 2000; 85: 1946-1950. [DOI:10.1210/jcem.85.5.6567]
31. Kus I, Sarsilmaz M, Canpolat S, Yilmaz B, Kelestimur H, Akpolat N, et al. Immunohistochemical, histological and ultrastructural evaluation of the effects of leptin on testes in mice. Arch Androl 2005; 51: 395-405. [DOI:10.1080/014850190944375]
32. Baldelli R, Dieguez C, Casanueva FF. The role of leptin in reproduction: Experimental and clinical aspects. Ann Med 2002; 34: 5-18. [DOI:10.1080/078538902317338599]
33. Barron J, Petrilli F, Strath L, McCaffrey R. Successful interventions for smoking cessation in pregnancy.MCN Am J Matern Child Nurs 2007; 32:42-47. [DOI:10.1097/00005721-200701000-00009]
34. Sofikitis N, Takenaka M, Kanakas N, Papadopoulos H, Yamamoto Y, Drakakis P, et al. Effects of cotinine on sperm motility, membrane function, and fertilizing capacity in vitro. Urol Res 2000; 28:370-375. [DOI:10.1007/s002400000138]
35. Gaur DS, Talekar M, Pathak VP. Effect of cigarette smoking on semen quality of infertile men.Singapore Med J 2007; 48: 119-123.
36. Ozgur K, Isikoglu M, Seleker M, Donmez L. Semen quality of smoking and non-smoking men in infertile couples in a Turkish population.Arch Gynecol Obstet 2005; 271:109-112. [DOI:10.1007/s00404-003-0572-z]
37. Ochedalski T, Lachowcz-ochedalska A, Dec W, czechowski B. Evaluating the effect of smoking tobacco on some semen parameters in men of reproductive age. Ginekol pol 1994; 65: 80-86.
38. Rosevear SK, Holt DW, Lee TD, Ford WC, Wardle PG, Hull MG. Smoking and decreased fertilization rates in-vitro. Lancet 1992; 340: 1195-1196. [DOI:10.1016/0140-6736(92)92895-M]
39. Holzki G, Gall H, Hermann J. Cigarette smoking and sperm quality. Andrologia 1991; 23: 141-144. [DOI:10.1111/j.1439-0272.1991.tb02517.x]
40. Vine MF, Tse CK, Hu P, Truong KY. Cigarette smoking and semen quality. Fertil Steril 1996; 65: 835-842. [DOI:10.1016/S0015-0282(16)58223-5]
41. Shi Q, Ko E, Barclay L, Hoang T, Rademaker A, Martin R. Cigarette smoking and aneuploidy in human sperm .Mol Reprod Dev 2001; 59: 417-421. [DOI:10.1002/mrd.1048]
42. Evans HJ, Fletcher J, Torrance M, Hargreave TB. Sperm abnormalities and cigarette smoking. Lancet 1991; 1: 627. [DOI:10.1016/S0140-6736(81)91550-6]
43. EL Mulla KF, Konh FM, EL Behiery AH, schill WB. The effect of smoking and varicocele on human sperm acrosin activity and acrosome reaction. HUM Reprod 1995; 10: 3190-3194. [DOI:10.1093/oxfordjournals.humrep.a135885]
44. Fraga CG, Motchnik PA, Wyrobek AJ, Rempel DM, Ames BN. Smoking and low antioxidant levels increase oxidative damage to sperm DNA. Mutat Res 1996; 351:199-203. [DOI:10.1016/0027-5107(95)00251-0]
45. Dikshit Rk, Buch JG, Mansuri SM. Effect of Tobacco consumption on semen quality of a population of hypofertile males. Fertil Steril 1987; 48: 334. [DOI:10.1016/S0015-0282(16)59369-8]
46. Steinman N, Gamzu R, Yogev L, Botchan A, Schreiber L, Yavetz H. Serum leptin concentrations are higher in azoospermic than in normozoospermic men. Fertil Steril 2001; 75: 821-822. [DOI:10.1016/S0015-0282(01)01707-1]
47. Trummer H, Haberman H, Haas J, pummer K. The impact of cigarette smoking on human semen parameters and hormones. HUM Reprod 2002; 17: 1554-1559. [DOI:10.1093/humrep/17.6.1554]
48. Lado-Abeal J, Norman RL. Leptin and reproductive function in males. Semin Reprod Med 2002; 20:145-152. [DOI:10.1055/s-2002-32506]
49. Rosenberg J, Benowitz NL, Javob P, Wilson KM. Disposition kinetics and effects of nicotine. Clin pharmacol Ther 1980; 28: 5517-5522. [DOI:10.1038/clpt.1980.196]
50. Wong WY, Thomas CM, Merkus HM, Zielhuis GA, Doesburg WH, Steegers-Theunissen RP. Cigarette smoking and the risk of male factor subfertility: minor association between cotinine in seminal plasma and semen morphology. Fertil Steril 2001; 75:1039-1040.
51. Bado A, Levasseur S, Attoub S, Kermorgant S, Laigneau JP, Bortoluzzi MN,et al . The stomach is a source of leptin. Nature 1998; 394:790-793. [DOI:10.1038/29547]
52. Wang J, Liu R, Hawkins M, Barzilai N, Rossetti L. A nutrient-sensing pathway regulates leptin gene expression in muscle and fat. Nature 1998;393:684-688. [DOI:10.1038/31474]
53. Reddy A, Sood A, Rust PF, Busby JE, Varn E, Mathur RS, et al. The effect of nicotine on in-vitro sperm motion characteristics. J Assist Repord Genet 1995; 12:217-223. [DOI:10.1007/BF02211802]
54. Vine MF, Margolin BH, Morrison HI, Hulka BS. Cigarette smoking and sperm density: a meta-analysis. Fertil Steril. 1994; 61:35-43. [DOI:10.1016/S0015-0282(16)56450-4]
55. Baldelli R, Dieguez C, Casanueva FF. The role of leptin in reproduction: experimental and clinical aspects. Ann Med 2002; 34:5-18. [DOI:10.1080/078538902317338599]
56. Dundar NO, Anal O, Dundar B, Ozkan H, Caliskan S, Büyükgebiz A. Longitudinal investigation of the relationship between breast milk leptin levels and growth in breast-fed infants. J Pediatr Endocrinol Metab 2005; 18:181-187. [DOI:10.1515/JPEM.2005.18.2.181]
57. Makler A, Reiss J, Stoller J, Blumenfeld Z, Brandes JM. Use of a sealed minichamber for direct observation and evaluation of the in vitro effect of cigarette smoke on sperm motility. Fertil Steril 1993; 59: 645-651. [DOI:10.1016/S0015-0282(16)55813-0]
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