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Volume 16, Issue 7 (July 2018)
IJRM 2018, 16(7): 447-454 |
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Khalaji N, Namyari M, Rasmi Y, Pourjabali M, Chodari L. Protective effect of curcumin on fertility of rats after exposure to compact fluorescent lamps: An experimental study. IJRM 2018; 16 (7) :447-454
URL: http://ijrm.ir/article-1-1169-en.html
URL: http://ijrm.ir/article-1-1169-en.html
1- Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran
2- Urmia University of Medical Sciences, Urmia, Iran
3- Department of Clinical Biochemistry, Urmia University Medical Sciences, Urmia, Iran
4- Department of Pathology, Urmia University of Medical Sciences, Urmia, Iran
5- Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran , lchodari@yahoo.com
2- Urmia University of Medical Sciences, Urmia, Iran
3- Department of Clinical Biochemistry, Urmia University Medical Sciences, Urmia, Iran
4- Department of Pathology, Urmia University of Medical Sciences, Urmia, Iran
5- Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran , lchodari@yahoo.com
Abstract: (2813 Views)
Background: Testicular function is modified by maturational gonadostatic control highly susceptible to negative physiologic niche-altering factors like UV-rays.
Objective: This study was performed in order to uncover new aspects of Compact Florescent Lamps (CFLs) induced damages on the testicular tissue of rats and evaluating the effect of curcumin on testis of rats after exposure to compact florescent Lamps.
Materials and Methods: Twenty-four adult male Albino rats were randomly divided into three groups: control group (ethyl oleate 0.2 ml, IP, for 45 days, without CFLs exposure), fluorescent group (ethyl oleate 0.2 ml, IP, daily and treated with 12 hr CFLs exposure for 45 days) and curcumin group (curcumin 20 μ M, IP along with 12 hr CFLs exposure for 45 days). The rats were anesthetized at the end of the experiment. Gonadotropin hormones and prolactin levels were measured; Histopathological and histomorphometrical analysis of the testis was carried out.
Results: Results of this study showed that CFLs significantly decreased serum levels of follicle stimulating hormone, prolactin, testicular weight, sperm motility, TDI, and SPI. Furthermore, CFLs had no effect on serum levels of luteinizing hormone and sperm count and also, increased abnormal sperm shapes. Our results also showed that curcumin supplementation following CFLs reversed these alterations.
Conclusion: These results strongly suggest that CFLs severely impairs testis while curcumin as an antioxidant had protective effects on undesirable effects in testis induced by CFLs.
Objective: This study was performed in order to uncover new aspects of Compact Florescent Lamps (CFLs) induced damages on the testicular tissue of rats and evaluating the effect of curcumin on testis of rats after exposure to compact florescent Lamps.
Materials and Methods: Twenty-four adult male Albino rats were randomly divided into three groups: control group (ethyl oleate 0.2 ml, IP, for 45 days, without CFLs exposure), fluorescent group (ethyl oleate 0.2 ml, IP, daily and treated with 12 hr CFLs exposure for 45 days) and curcumin group (curcumin 20 μ M, IP along with 12 hr CFLs exposure for 45 days). The rats were anesthetized at the end of the experiment. Gonadotropin hormones and prolactin levels were measured; Histopathological and histomorphometrical analysis of the testis was carried out.
Results: Results of this study showed that CFLs significantly decreased serum levels of follicle stimulating hormone, prolactin, testicular weight, sperm motility, TDI, and SPI. Furthermore, CFLs had no effect on serum levels of luteinizing hormone and sperm count and also, increased abnormal sperm shapes. Our results also showed that curcumin supplementation following CFLs reversed these alterations.
Conclusion: These results strongly suggest that CFLs severely impairs testis while curcumin as an antioxidant had protective effects on undesirable effects in testis induced by CFLs.
Type of Study: Original Article |
References
1. Greil AL, Slauson‐Blevins K, McQuillan J. The experience of infertility: a review of recent literature. Soc Health Illn 2010; 32: 140-162. [DOI:10.1111/j.1467-9566.2009.01213.x]
2. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl 2004; 6: 259-268.
3. Meeker JD, Godfrey‐Bailey L, Hauser R. Relationships between serum hormone levels and semen quality among men from an infertility clinic. J Androl 2007; 28: 397-406. [DOI:10.2164/jandrol.106.001545]
4. Ben Khelifa M, Coutton C, Zouari R, Karaouzène T, Rendu J, Bidart M, et al. Mutations in DNAH1, which encodes an inner arm heavy chain dynein, lead to male infertility from multiple morphological abnormalities of the sperm flagella. Am J Hum Genet 2014; 94: 95-104. [DOI:10.1016/j.ajhg.2013.11.017]
5. Durairajanayagam D. Lifestyle causes of male infertility. Arab J Urol 2018; 16: 10-20. [DOI:10.1016/j.aju.2017.12.004]
6. Cho Y, Ryu SH, Lee BR, Kim KH, Lee E, Choi J. Effects of artificial light at night on human health: A literature review of observational and experimental studies applied to exposure assessment. Chronobiol Int 2015; 32: 1294-1310. [DOI:10.3109/07420528.2015.1073158]
7. Klein RS, Werth VP, Dowdy JC, Sayre RM. Analysis of compact fluorescent lights for use by patients with photosensitive conditions. Photochem Photobiol 2009; 85: 1004-1010. [DOI:10.1111/j.1751-1097.2009.00540.x]
8. Zhu YZ, Huang SH, Tan BK, Sun J, Whiteman M, Zhu YC. Antioxidants in Chinese herbal medicines: a biochemical perspective. Nat Prod Rep 2004; 21: 478-489. [DOI:10.1039/b304821g]
9. Yun SS, Kim SP, Kang MY, Nam SH. Inhibitory effect of curcumin on liver injury in a murine model of endotoxemic shock. Biotechnol Lett 2010; 32: 209-214. [DOI:10.1007/s10529-009-0153-8]
10. Ilbey YO, Ozbek E, Cekmen M, Simsek A, Otunctemur A, Somay A. Protective effect of curcumin in cisplatin-induced oxidative injury in rat testis: mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways. Hum Reprod 2009; 24: 1717-1725. [DOI:10.1093/humrep/dep058]
11. Abarikwu SO, Akiri OF, Durojaiye MA, Alabi AF. Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes. J Steroid Biochem Mol Biol 2014; 143: 49-60. [DOI:10.1016/j.jsbmb.2014.02.008]
12. Bucak MN, Başpınar N, Tuncer PB, Coyan K, Sarıözkan S, Akalın PP, et al. Effects of curcumin and dithioerythritol on frozen‐thawed bovine semen. Andrologia 2012; 44 (Suppl.): 102-109. [DOI:10.1111/j.1439-0272.2010.01146.x]
13. Goettsch W, Garssen J, de Gruijl FR, Dortant P, van Loveren H. Methods for exposure of laboratory animals to ultraviolet radiation. Lab Anim 1999; 33: 58-67. [DOI:10.1258/002367799780578507]
14. Abdel Aziz MT, El-Asmar MF, El-Ibrashy IN, Rezq AM, Al-Malki AL, Wassef MA, et al. Effect of novel water soluble curcumin derivative on experimental type-1 diabetes mellitus (short term study). Diabetol Metab Syndr 2012; 4: 30. [DOI:10.1186/1758-5996-4-30]
15. Besley MA, Moghissi KS, Eliarson R, Paulsen CA, Gallegosm AJ, Prasad MR. Laboratory manual for the examination of human semen and semen cervical mucus interaction. WHO Press concern: Singapore, 1980.
16. Wyrobek AJ, Bruce WR. Chemical induction of sperm abnormalities in mice. Proc Nati Acad Sci 1975; 72: 4425-4429. [DOI:10.1073/pnas.72.11.4425]
17. Jalali AS, Hasanzadeh S, Malekinejad H. Crataegus monogyna aqueous extract ameliorates cyclophosphamide-induced toxicity in rat testis: stereological evidences. Acta Med Iran 2012; 50: 1-8.
18. Hartman PE, Biggley WH. Breakthrough of ultraviolet light from various brands of fluorescent lamps: Lethal effects on DNA repair‐defective bacteria. Environ Mol Mutagen 1996; 27: 306-313.
https://doi.org/10.1002/(SICI)1098-2280(1996)27:4<306::AID-EM5>3.0.CO;2-A [DOI:10.1002/(SICI)1098-2280(1996)27:43.0.CO;2-A]
19. Falkenbach A, Lorenzen H, Althoff P, Bühring M. Unchanged response to stimulation of pituitary hormone release after serial UV irradiation in men. Eur J Appl Physiol Occup Physiol 1997; 75: 449-454. [DOI:10.1007/s004210050187]
20. Recio R, Ocampo-Gómez G, Morán-Martínez J, Borja-Aburto V, López-Cervantes M, Uribe M, et al. Pesticide exposure alters follicle-stimulating hormone levels in Mexican agricultural workers. Environ Health Perspect 2005; 113: 1160-1163. [DOI:10.1289/ehp.7374]
21. Gultekin FA, Bakkal BH, Guven B, Tasdoven I, Bektas S, Can M, et al. Effects of ozone oxidative preconditioning on radiation-induced organ damage in rats. J Radiat Res 2013; 54: 36-44. [DOI:10.1093/jrr/rrs073]
22. De Jager TL, Cockrell AE, Du Plessis SS. Ultraviolet Light Induced Generation of Reactive Oxygen Species. Adv Exp Med Biol 2017; 996: 15-23. [DOI:10.1007/978-3-319-56017-5_2]
23. Tousson E, Hafez E, Masoud A, Hassan AA. Abrogation by curcumin on testicular toxicity induced by Cisplatin in rats. J Cancer Res Treat 2014; 2: 64-68.
24. Sadoughi D. Effect of Curcumin and Low Frequency Electromagnetic Field on the Hormones of Pituitary-Gonad Axis in Male Diabetic Rats. Horizon Med Sci 2017; 23: 27-33. [DOI:10.18869/acadpub.hms.23.1.27]
25. Zoodfekr L, Matin Homaee H, Tarverdizadeh B. The Effect of an Aerobic Training Course and Consumption of Curcumin on Prostaglandin E2 and Prolactin Levels in Women with Premenstrual Syndrome. Iran J Endocrinol Metabol 2018; 19: 444-451.
26. Wang R, Li Y-H, Xu Y, Li Y-B, Wu H-L, Guo H, et al. Curcumin produces neuroprotective effects via activating brain-derived neurotrophic factor/TrkB-dependent MAPK and PI-3K cascades in rodent cortical neurons. Prog Neuropsychopharmacol Biol Psychiatr 2010; 34: 147-153. [DOI:10.1016/j.pnpbp.2009.10.016]
27. Smith LB, Walker WH. The regulation of spermatogenesis by androgens. Semin Cell Dev Biol 2014; 30: 2-13. [DOI:10.1016/j.semcdb.2014.02.012]
28. McLachlan RI, O'donnell L, Meachem SJ, Stanton PG, de Kretser DM, Pratis K, et al. Identification of specific sites of hormonal regulation in spermatogenesis in rats, monkeys, and man. Recent Prog Horm Res 2002; 57: 149-179. [DOI:10.1210/rp.57.1.149]
29. Dombrowicz D, Sente B, Closset J, Hennen G. Dose-dependent effects of human prolactin on the immature hypophysectomized rat testis. Endocrinology 1992; 130: 695-700.
30. Segal S, Yaffe H, Laufer N, Ben-David M. Male hyperprolactinemia: effects on fertility. Fertil Steril 1979; 32: 556-561. [DOI:10.1016/S0015-0282(16)44359-1]
31. Torres ER, Abad C, Pi-ero S, Proverbio T, Marín R, Proverbio F, et al. Effect of ultraviolet C irradiation on human sperm motility and lipid peroxidation. Int J Radiat Biol 2010; 86: 187-193. [DOI:10.3109/09553000903419288]
32. Gouda ZA, Selim AO. A possible correlation between the testicular structure and short photoperiod exposure in young albino rats: light and electron microscopic study. Egypt J Histol 2013; 36: 28-38. [DOI:10.1097/01.EHX.0000423980.95382.0c]
33. Skjærvø GR, Fossøy F, Røskaft E. Solar activity at birth predicted infant survival and women's fertility in historical Norway. Proc Biol Sci 2015; 282: 20142032. [DOI:10.1098/rspb.2014.2032]
34. Yamasaki H, Sandrof MA, Boekelheide K. Suppression of radiation-induced testicular germ cell apoptosis by 2, 5-hexanedione pretreatment. I. Histopathological analysis reveals stage dependence of attenuated apoptosis. Toxicol Sci 2010; 117: 449-456. [DOI:10.1093/toxsci/kfq203]
35. Nagata M. Inflammatory cells and oxygen radicals. Curr Drug Targets Inflamm Allergy 2005; 4: 503-504. [DOI:10.2174/1568010054526322]
36. Zini A, de Lamirande E, Gagnon C. Reactive oxygen species in semen of infertile patients: levels of superoxide dismutase‐and catalase‐like activities in seminal plasma and spermatozoa. Int J Androl 1993; 16: 183-188. [DOI:10.1111/j.1365-2605.1993.tb01177.x]
37. Wang X, Sharma RK, Sikka SC, Thomas AJ Jr, Falcone T, Agarwal A. Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility. Fertil Steril 2003; 80: 531-535. [DOI:10.1016/S0015-0282(03)00756-8]
38. Shiva M, Gautam AK, Verma Y, Shivgotra V, Doshi H, Kumar S. Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clin Biochem 2011; 44: 319-324. [DOI:10.1016/j.clinbiochem.2010.11.009]
39. Saraswat S, Kharche S, Jindal S. Impact of Reactive Oxygen Species on Spermatozoa: A Balancing Act between Beneficial and Detrimental Effects. Iran J Applied Anim Sci 2014; 4: 247-255.
40. Hess RA, de Franca LR. Spermatogenesis and cycle of the seminiferous epithelium. Mol Mech Spermatogen 2009: 1-15. [DOI:10.1007/978-0-387-09597-4_1]
41. Al-Rubaei ZM, Mohammad TU, Ali LK. Effects of local curcumin on oxidative stress and total antioxidant capacity in vivo study. Pak J Biol Sci 2014; 17: 1237-1241. [DOI:10.3923/pjbs.2014.1237.1241]
42. Ghosh S, Bhattacharyya S, Rashid K, Sil PC. Curcumin protects rat liver from streptozotocin-induced diabetic pathophysiology by counteracting reactive oxygen species and inhibiting the activation of p53 and MAPKs mediated stress response pathways. Toxicol Rep 2015; 2: 365-376. [DOI:10.1016/j.toxrep.2014.12.017]
43. Maithili Karpaga Selvi N, Sridhar MG, Swaminathan RP, Sripradha R. Curcumin attenuates oxidative stress and activation of redox-sensitive kinases in high fructose-and high-fat-fed male Wistar rats. Sci Pharm 2014; 83: 159-175. [DOI:10.3797/scipharm.1408-16]
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