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Volume 18, Issue 1 (January 2020)
IJRM 2020, 18(1): 21-32 |
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Zarei H, Karimpour A, Khalatbary A R, Talebpour Amiri F. Homing of adipose stem cells on the human amniotic membrane as a scaffold: A histological study. IJRM 2020; 18 (1) :21-32
URL: http://ijrm.ir/article-1-1191-en.html
URL: http://ijrm.ir/article-1-1191-en.html
1- Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
2- Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
2- Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract: (2460 Views)
Background: The human amniotic membrane (HAM) is a suitable and effective scaffold for cell culture and delivery, and adipose-derived stem cells (ADSCs) are an important source of stem cells for transplantation and chondrogenic differentiation.
Objective: To assess the practicability of a cryopreserved HAM as a scaffold in cell proliferation and differentiation in vitro.
Materials and Methods: In this experimental study, adipose tissue samples were harvested from the inguinal region of male patients aged 15-30 years. Flow cytometry was used to identify CD31, CD45, CD90, and CD105 markers in adipose stem cells. HAM was harvested from donor placenta after cesarean section, washed, trypsin-based decellularized trypsinized decellularized, and used as a scaffold via three methods: 1) ADSCs were differentiated into chondrocytes on cell culture flasks (monolayer method), and after 14 days of culture, the cells were transferred and cultured on both sides of the HAM; 2) ADSCs were cultured and differentiated directly on both sides of the HAM for 14 days (scaffold-mediated differentiation); and 3) chondrocytes were differentiated with micromass culture for 14 days, transferred on HAM, and tissue slides were histologically analyzed qualitatively.
Results: Flow cytometry confirmed the presence of mesenchymal stem cells. Histological findings revealed that the cells adhered and grew well on the stromal layer of HAM. Among the three methods, scaffold-mediated differentiation of ADSCs showed the best results.
Conclusion: ADSCs have excellent attachment, viability, and differentiation capacity in the stromal side of HAM. Additionally, the direct culture and differentiation of ADSCs on HAM is more suitable than the culture of differentiated cells on HAM.
Objective: To assess the practicability of a cryopreserved HAM as a scaffold in cell proliferation and differentiation in vitro.
Materials and Methods: In this experimental study, adipose tissue samples were harvested from the inguinal region of male patients aged 15-30 years. Flow cytometry was used to identify CD31, CD45, CD90, and CD105 markers in adipose stem cells. HAM was harvested from donor placenta after cesarean section, washed, trypsin-based decellularized trypsinized decellularized, and used as a scaffold via three methods: 1) ADSCs were differentiated into chondrocytes on cell culture flasks (monolayer method), and after 14 days of culture, the cells were transferred and cultured on both sides of the HAM; 2) ADSCs were cultured and differentiated directly on both sides of the HAM for 14 days (scaffold-mediated differentiation); and 3) chondrocytes were differentiated with micromass culture for 14 days, transferred on HAM, and tissue slides were histologically analyzed qualitatively.
Results: Flow cytometry confirmed the presence of mesenchymal stem cells. Histological findings revealed that the cells adhered and grew well on the stromal layer of HAM. Among the three methods, scaffold-mediated differentiation of ADSCs showed the best results.
Conclusion: ADSCs have excellent attachment, viability, and differentiation capacity in the stromal side of HAM. Additionally, the direct culture and differentiation of ADSCs on HAM is more suitable than the culture of differentiated cells on HAM.
Type of Study: Original Article |
Subject:
Stem Cell & Cloning
References
1. Hootman JM, Helmick CG, Barbour KE, Theis KA, Boring MA. Updated projected prevalence of self‐reported doctor‐diagnosed arthritis and arthritis‐attributable activity limitation among US adults, 2015-2040. Arthritis Rheumatol 2016; 68: 1582-1587. [DOI:10.1002/art.39692] [PMID] [PMCID]
2. Bade MJ, Kohrt WM, Stevens-Lapsley JE. Outcomes before and after total knee arthroplasty compared to healthy adults. J Orthop Sports Phys Ther 2010; 40: 559-567. [DOI:10.2519/jospt.2010.3317] [PMID] [PMCID]
3. Sadoghi P, Liebensteiner M, Agreiter M, Leithner A, Böhler N, Labek G. Revision surgery after total joint arthroplasty: a complication-based analysis using worldwide arthroplasty registers. J Arthroplasty 2013; 28: 1329-1332. [DOI:10.1016/j.arth.2013.01.012] [PMID]
4. Oseni AO, Butler PE, Seifalian AM. Optimization of chondrocyte isolation and characterization for large-scale cartilage tissue engineering. J Surg Res 2013; 181: 41-48. [DOI:10.1016/j.jss.2012.05.087] [PMID]
5. Zheng M-H, Willers C, Kirilak L, Yates P, Xu J, Wood D, et al. Matrix-induced autologous chondrocyte implantation (MACI®): biological and histological assessment. Tissue Eng 2007; 13: 737-746. [DOI:10.1089/ten.2006.0246] [PMID]
6. Almeida HV, Liu Y, Cunniffe GM, Mulhall KJ, Matsiko A, Buckley CT, et al. Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells. Acta Biomater 2014; 10: 4400-4409. [DOI:10.1016/j.actbio.2014.05.030] [PMID]
7. Can A, Karahuseyinoglu S. Concise review: human umbilical cord stroma with regard to the source of fetus‐derived stem cells. Stem Cells 2007; 25: 2886-2895. [DOI:10.1634/stemcells.2007-0417] [PMID]
8. Benders KE, van Weeren PR, Badylak SF, Saris DB, Dhert WJ, Malda J. Extracellular matrix scaffolds for cartilage and bone regeneration. Trends Biotechnol 2013; 31: 169-176. [DOI:10.1016/j.tibtech.2012.12.004] [PMID]
9. Cheng N-C, Estes BT, Awad HA, Guilak F. Chondrogenic differentiation of adipose-derived adult stem cells by a porous scaffold derived from native articular cartilage extracellular matrix. Tissue Eng Part A 2009; 15: 231-241. [DOI:10.1089/ten.tea.2008.0253] [PMID] [PMCID]
10. Focaroli S, Teti G, Salvatore V, Durante S, Belmonte MM, Giardino R, et al. Chondrogenic differentiation of human adipose mesenchimal stem cells: influence of a biomimetic gelatin genipin crosslinked porous scaffold. Microsc Res Tech 2014; 77: 928-934 [DOI:10.1002/jemt.22417] [PMID]
11. Gholipourmalekabadi M, Sameni M, Radenkovic D, Mozafari M, Mossahebi‐Mohammadi M, Seifalian A. Decellularized human amniotic membrane: how viable is it as a delivery system for human adipose tissue‐derived stromal cells? Cell Prolif 2016; 49: 115-121. [DOI:10.1111/cpr.12240] [PMID]
12. Mahmoudi MR, Talebpour FA, Mirhoseini M, Ghasemi M, Mirzaei M, Mosaffa N. Application of Allogeneic Fibroblast Cultured on Acellular Amniotic Membrane for Full-thickness Wound Healing in Rats. Wounds 2016; 28: 14-19.
13. Jerman UD, Veranič P, Cirman T, Kreft ME. Amniotic membrane scaffold enriched with bladder fibroblasts promotes establishment of highly differentiated urothelium. InEuropean Microscopy Congress 2016: Proceedings 2016; Nov 30: 97-98. Wiley Online Library. [DOI:10.1002/9783527808465.EMC2016.5736]
14. Sanluis-Verdes A, Vilar MTY-P, García-Barreiro JJ, García-Camba M, Ibáñez JS, Doménech N, et al. Production of an acellular matrix from amniotic membrane for the synthesis of a human skin equivalent. Cell Tissue Bank 2015; 16: 411-423. [DOI:10.1007/s10561-014-9485-2] [PMID]
15. Prabhasawat P. Amniotic Membrane: A Treatment for Prevention of Blindness from Various Ocular Diseases. Siriraj Med J 2017; 59: 139-141.
16. Jin CZ, Park SR, Choi BH, Lee KY, Kang CK, Min BH. Human amniotic membrane as a delivery matrix for articular cartilage repair. Tissue Eng 2007; 13: 693-702. [DOI:10.1089/ten.2006.0184] [PMID]
17. Liu P, Guo L, Zhao D, Zhang Z, Kang K, Zhu R, et al. Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits. Genet Mol Res 2014; 13: 7992-8001. [DOI:10.4238/2014.September.29.12] [PMID]
18. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8: 315-317. [DOI:10.1080/14653240600855905] [PMID]
19. De Francesco F, Mannucci S, Conti G, Dai Prè E, Sbarbati A, Riccio M. A Non-Enzymatic Method to Obtain a Fat Tissue Derivative Highly Enriched in Adipose Stem Cells (ASCs) from Human Lipoaspirates: Preliminary Results. Int J Mol Sci 2018; 19: E2061. [DOI:10.3390/ijms19072061] [PMID] [PMCID]
20. Lee HJ, Choi BH, Min BH, Park SR. Changes in surface markers of human mesenchymal stem cells during the chondrogenic differentiation and dedifferentiation processes in vitro. Arthritis Rheum 2009; 60: 2325-2332. [DOI:10.1002/art.24786] [PMID]
21. Karimpour Malekshah A, Talebpour Amiri F, Ghaffari E, Alizadeh A, Jamalpoor Z, Mirhosseini M, et al. Growth and chondrogenic differentiation of mesenchymal stem cells derived from human adipose tissue on chitosan scaffolds. J Babol Univ Med Sci 2016; 18: 32-38.
22. Davis JS. Skin transplantation. Johns Hopkins Hospital Reports 1910; 15: 307-396.
23. Arrizabalaga JH, Nollert MU. The Human Amniotic Membrane: A Versatile Scaffold for Tissue Engineering. ACS Biomater Sci Eng 2018; 7: 2226-2236. [DOI:10.1021/acsbiomaterials.8b00015]
24. Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian AM. Properties of the amniotic membrane for potential use in tissue engineering. Eur Cells Mater. 2008;15:88-99. [DOI:10.22203/eCM.v015a07] [PMID]
25. Lim R. Concise Review: Fetal Membranes in Regenerative Medicine: New Tricks from an Old Dog? Stem Cells Transl Med 2017; 6: 1767-1776. [DOI:10.1002/sctm.16-0447] [PMID] [PMCID]
26. Arrizabalaga JH, Nollert MU. Human amniotic membrane: A versatile scaffold for tissue engineering. ACS Biomater Sci Eng 2018; 4: 2226-2236. [DOI:10.1021/acsbiomaterials.8b00015]
27. Díaz-Prado S, Rendal-Vázquez ME, Muiños-López E, Hermida-Gómez T, Rodríguez-Cabarcos M, Fuentes-Boquete I, et al. Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair. Cell Tissue Bank 2010; 11: 183-195. [DOI:10.1007/s10561-009-9144-1] [PMID]
28. Chen YJ, Chung MC, Jane Yao CC, Huang CH, Chang HH, Jeng JH, et al. The effects of acellular amniotic membrane matrix on osteogenic differentiation and ERK1/2 signaling in human dental apical papilla cells. Biomaterials 2012; 33: 455-463. [DOI:10.1016/j.biomaterials.2011.09.065] [PMID]
29. Yang L, Shirakata Y, Tokumaru S, Xiuju D, Tohyama M, Hanakawa Y, et al. Living skin equivalents constructed using human amnions as a matrix. J Dermatol Sci 2009; 56: 188-195. [DOI:10.1016/j.jdermsci.2009.09.009] [PMID]
30. Nogami M, Kimura T, Seki S, Matsui Y, Yoshida T, Koike-Soko C, et al. A Human Amnion-Derived Extracellular Matrix-Coated Cell-Free Scaffold for Cartilage Repair: In Vitro and In Vivo Studies. Tissue Eng Part A 2016; 22: 680-688. [DOI:10.1089/ten.tea.2015.0285] [PMID]
31. Ghaffari E, Talebpour Amiri F, Karimpour Malekshah A, Mirhosseini M, Barzegarnejad A. [Comparing the Chondrogenesis Potential of Human Adipose-derived Stem Cells in Monolayer and Micromass Culture Systems]. J Mazandaran Univ Med Sci 2016; 25: 37-47. (in Persian)
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