Volume 6, Issue 5 (7-2008)                   IJRM 2008, 6(5): 199-204 | Back to browse issues page

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Javed A, Rezaei-Zarchi S, Anvari M, Javeed Ghani M, Barzegari Firouzabadi F, Jamil A, et al . Determination of effective dosage of FSH and hCG in the maturation of preantral follicles and enclosed oocytes in mice. IJRM 2008; 6 (5) :199-204
URL: http://ijrm.ir/article-1-124-en.html
Determination of effective dosage of FSH and hCG in the maturation of preantral follicles and enclosed oocytes in mice
Aisha Javed1 , Saeed Rezaei-Zarchi2 , Morteza Anvari1 , Madiha Javeed Ghani3 , Fatemeh Barzegari Firouzabadi2 , Amer Jamil4 , Seyed Mehdi Kalantar * 5, Habibollah Nazem2
1- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Department of Biology, Payam-e-Noor University, Yazd, Iran
3- Department of Bioinformatics, Government College University, Faisalabad, Pakistan
4- Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan
5- Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , smkalantar@yahoo.com
Abstract:   (2921 Views)
Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM is an intellectual, scientific and clinical challenge with a number of potential applications.
Objective: The effect of hCG was evaluated on the timing and regulation of in vitro ovulation for the Syrian mice oocytes in the presence and absence of FSH. 
Materials and Methods: Preantral follicles, isolated from the ovaries of 6 weeks-old mice, were cultured in TCM-199 medium. The effect of 10-200 mIU/ml FSH and 1.5 IU/ml hCG was seen on the follicle maturation, as well as the changes in ovulation capacity of enclosed oocytes, after the incubation period of 6 days at 37 °C, 92% humidity and 5% CO2 in air.
Results: 100 mIU/ml FSH showed increased follicle diameter, survival, germinal vesicle breakdown (GVBD) and oocyte maturation rates (p<0.0001). Significantly higher number of follicles showed cumulus attachment when ovulation started within 16-24 hours post hCG (97% and 80% respectively; p<0.0001) as compared to the cultures without hCG or when the ovulation time increased from 24 hours post hCG. Combination of FSH and hCG showed 97% (p<0.0001) ovulation as compared to that seen for FSH-containing medium only (81%) or control (10%).
Conclusion: The combined administration of 1.5 IU/ml hCG and 100 mIU/ml FSH induces the in vitro follicle maturation, ovulation capacity and proper timing of mice oocytes. 
Keywords: Follicle stimulating hormone, HCG, Preantral follicles, Oocyte maturation, GVBD.
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Type of Study: Original Article |
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