Volume 7, Issue 1 (7-2009)                   IJRM 2009, 7(1): 29-0 | Back to browse issues page

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Venkatesh S, Gurdeep Singh M, Prasad Gupta N, Kumar R, Deecaraman M, Dada R. Correlation of sperm morphology and oxidative stress in infertile men. IJRM 2009; 7 (1) :29-0
URL: http://ijrm.ssu.ac.ir/article-1-133-en.html
1- Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, AIIMS, New Delhi, India
2- Department of Pathology, Air Force Central Medical Establishment, New Delhi, India
3- Department of Urology, AIIMS, New Delhi, India
4- Dr. MGR University, Maduravoyal, Chennai, India
5- Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, AIIMS, New Delhi, India , rima_dada@rediffmail.com
Abstract:   (2595 Views)
Background: Excess reactive oxygen species (ROS) in the semen is believed to affect fertility in men. Morphologically abnormal sperms and their relation to seminal oxidative stress in infertile and subfertile men are not clear.
Objective: To correlate various sperm morphological defects with seminal oxidative stress in infertile and subfertile men.
Materials and Methods: The study included 25 primary, 21 secondary infertile men of idiopathic infertility and 15 fertile controls. Standard semen analysis was performed according to WHO (1999) guidelines. Sperm inter-morphological defects were evaluated in 100 sperms per sample by Giemsa staining. ROS in spermatozoa was measured by the chemiluminescence assay.
Results: Significant difference in percent sperm amorphous head was found between secondary infertile group and control men. The study showed a significantly higher percent spermatozoa with residual cytoplasm between primary [11.61 (6.6, 3.9)], secondary [7.49 (0.8, 13)] and fertile controls [2.44 (0.8, 3.7)] similar to sperm count, percent sperm progressive motility, and ROS levels. A non significant but strong positive correlation (r=0.3479, p=0.0884) between percent cytoplasmic retained spermatozoa and ROS levels was observed in the primary infertile group. However, no correlation between other sperm morphological defects and oxidative stress was observed.
Conclusion: Sperm morphology was not found to be associated with oxidative stress in the present study. However, retained cytoplasmic residues in the sperm may be an important source of ROS in both primary and secondary infertile men. These immature spermatozoa are believed to be associated with impaired fertility.
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Type of Study: Original Article |

References
1. Carlsen E, Giwercman A, Keiding N, Skakkebaek NE. Evidence for decreasing quality of semen during past 50 years. BMJ 1992; 305: 609-613. [DOI:10.1136/bmj.305.6854.609]
2. Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, et al. Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod 2001; 16: 1912-1921. [DOI:10.1093/humrep/16.9.1912]
3. Tremellen K. Oxidative stress and male infertility--a clinical perspective. Hum Reprod Update 2008; 14:243-258. [DOI:10.1093/humupd/dmn004]
4. Dada R, Kumar R, Sharma RK, Gupta NP, Gupta SK. et al. AZF deletion in varicocele cases with oligospermia. IJMS 2007; 61:505-510.
5. Ushijima C, Kumasako Y, Kihaile PE, Hirotsuru K, Utsunomiya T. Analysis of chromosomal abnormalities in human spermatozoa using multi-colour fluorescence in-situ hybridization. Hum Reprod 2000; 15:1107-1111. [DOI:10.1093/humrep/15.5.1107]
6. Meeker JD, Rossano MG, Protas B, Diamond MP, Puscheck E, Daly D, et al. Cadmium, Lead, and Other Metals in Relation to Semen Quality: Human Evidence for Molybdenum as a Male Reproductive Toxicant 2008;116:1473-1479.
7. Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF, Oehninger S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988; 49:112-117. [DOI:10.1016/S0015-0282(16)59660-5]
8. Oehninger S, Acosta AA, Morshedi M, Veeck L, Swanson RJ, Simmons K, et al. Corrective measures and pregnancy outcome in in vitro fertilization in patients with severe sperm morphology abnormalities. Fertil Steril 1988; 50:283-287. [DOI:10.1016/S0015-0282(16)60074-2]
9. Griveau JF, Le Lannou D. Reactive oxygen species and human spermatozoa: Physiology and pathology. Int J Androl 1997; 20:61-69. [DOI:10.1046/j.1365-2605.1997.00044.x]
10. Agarwal A, Nallela KP, Allamaneni SSR, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed online 2004;8:616-627 [DOI:10.1016/S1472-6483(10)61641-0]
11. Venkatesh S, Deecaraman M, Kumar R, Shamsi MB, Dada R. Reactive oxygen species and its role in the pathogenesis of mitochondrial DNA (mtDNA) mutations in male infertility. Ind J Med Res 2009. In Press
12. Chia SE, Tay SK, Lim ST. What constitutes a normal seminal analysis? Semen parameters of 243 fertile men. Hum Reprod 1998; 13:3394-3398. [DOI:10.1093/humrep/13.12.3394]
13. Guzick DS, Overstreet JW, Factor-Litvak P, Brazil CK, Nakajima ST, Coutifaris C, et al. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2001; 345:1388-1393. [DOI:10.1056/NEJMoa003005]
14. Nallella KP, Sharma RK, Aziz N, Agarwal A. Significance of sperm characteristics in the evaluation of male infertility. Fertil Steril 2006; 85:629-634. [DOI:10.1016/j.fertnstert.2005.08.024]
15. Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas AJ Jr, et al. Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril 2004; 81:349-354. [DOI:10.1016/j.fertnstert.2003.06.026]
16. Moein MR, Dehghani VO, Tabibnejad N, Vahidi S. Reactive Oxygen Species (ROS) level in seminal plasma of infertile men and healthy donors. Iranian Journal of Reproductive Medicine 2007; 5:51-55.
17. WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. 4 ed. Cambridge, UK: Cambridge University Press, 1999.
18. Athayde KS, Cocuzza M, Agarwal A, Krajcir N, Lucon AM, Srougi M, et al. Development of normal reference values for seminal reactive oxygen species and their correlation with leukocytes and semen parameters in a fertile population. J Androl 2007; 28:613-620. [DOI:10.2164/jandrol.106.001966]
19. Venkatesh S, Riaz AM, Kumar M, Shamsi MB, Tanwar M, Kumar R, et al. OXIDATIVE STRESS - A Marker of Male infertility. Obs and Gyn today 2009; 14:34-36.
20. Zarghami N, Khosrowbeygi A. Evaluation of Lipid Peroxidation as an Indirect Measure of Oxidative Stress in Seminal Plasma. Iranian Journal of Reproductive Medicine 2004; 2:34-39.
21. Said TM, Aziz N, Sharma RK, Lewis-Jones I, Thomas Jr AJ, Agarwal A. Novel association between sperm deformity index and oxidative stress-induced DNA damage in infertile male patients. Asian J Androl 2005; 7:121-126. [DOI:10.1111/j.1745-7262.2005.00022.x]
22. Said TM, Agarwal A, Sharma RK, Thomas AJ Jr., Sikka SC. Impact of sperm morphology on DNA damage caused by oxidative stress induced by beta-nicotinamide adenine dinucleotide phosphate. Fertil Steril 2005; 83:95-103. [DOI:10.1016/j.fertnstert.2004.06.056]
23. Alvarez J, Touchstone J, Blasco L, Storey B. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. J Androl 1987; 8: 338-348. [DOI:10.1002/j.1939-4640.1987.tb00973.x]
24. Andreyev AYu, Kushnareva YuE, Starkov AA. Mitochondrial Metabolism of Reactive Oxygen Species. Biochemistry (Moscow) 2005; 70:200-214. [DOI:10.1007/s10541-005-0102-7]
25. Nicopoullos JD, Gilling-Smith C, Almeida PA, Homa S, Norman-Taylor JQ, Ramsay JW. Sperm DNA fragmentation in subfertile men: the effect on the outcome of intracytoplasmic sperm injection and correlation with sperm variables. BJU Int 2008; 101:1553-1560. [DOI:10.1111/j.1464-410X.2008.07518.x]
26. Huang CC, Lin DP, Tsao HM, Cheng TC, Liu CH, Lee MS. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril 2005; 84:130-140. [DOI:10.1016/j.fertnstert.2004.08.042]
27. Muratori M, Piomboni P, Baldi E, Filimberti E, Pecchioli P, Moretti E, et al. Functional and Ultrastructural Features of DNA-Fragmented Human Sperm. Journal of Androl 2000; 21:903-912.
28. Fisher H, Aitken R. Comparative analysis of the ability of precursor germ cells and epididymal spermatozoa to generate reactive oxygen metabolites. J Exp Zool 1997; 277:390-400. https://doi.org/10.1002/(SICI)1097-010X(19970401)277:5<390::AID-JEZ5>3.0.CO;2-K [DOI:10.1002/(SICI)1097-010X(19970401)277:53.0.CO;2-K]
29. Gil-Guzman E, Ollero M, Lopez M, Sharma R, Alvarez J, Thomas AJ, et al. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 2001; 16:1922-1930. [DOI:10.1093/humrep/16.9.1922]
30. Kobayashi T, Jinno M, Sugimura K, Nozawa S, Sugiyama T, Iida E. Sperm morphological assessment based on strict criteria and in-vitro fertilization outcome. Hum Reprod 1991; 6:983-986. [DOI:10.1093/oxfordjournals.humrep.a137473]
31. Agarwal A, Nallella KP, Allamaneni SS, Said TM. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 2004; 8:616-627. [DOI:10.1016/S1472-6483(10)61641-0]
32. Lenzi A, Culasso F, Gandini L, Lombardo F , Dondero F. Placebocontrolled, double-blind, cross-over trial of glutathione therapy in male infertility. Human Reprod 1993; 8: 1657-1662. [DOI:10.1093/oxfordjournals.humrep.a137909]

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