International Journal of
Reproductive Biomedicine
Mon, Dec 30, 2024
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Volume 18, Issue 7 (July 2020)
IJRM 2020, 18(7): 501-508 |
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Charostad J, Mokhtari-Azad T, Yavarian J, Ghavami N, Seyed Khorrami S M, Behboodi E, et al . Detection of human herpes viruses 1-5 in miscarriage: A case-control study. IJRM 2020; 18 (7) :501-508
URL: http://ijrm.ir/article-1-1339-en.html
URL: http://ijrm.ir/article-1-1339-en.html
Javad Charostad1 
, Talat Mokhtari-Azad1 , Jila Yavarian1 , Nastaran Ghavami1 , Seyed Mahmood Seyed Khorrami2 , Emad Behboodi1 , Somayeh Jalilvand1 , Somayeh Shatizadeh Malekshahi2 , Nazanin-Zahra Shafiei-Jandaghi * 3
, Talat Mokhtari-Azad1 , Jila Yavarian1 , Nastaran Ghavami1 , Seyed Mahmood Seyed Khorrami2 , Emad Behboodi1 , Somayeh Jalilvand1 , Somayeh Shatizadeh Malekshahi2 , Nazanin-Zahra Shafiei-Jandaghi * 3
1- Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
2- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
3- Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. ,nz-shafiei@tums.ac.ir
2- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
3- Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. ,
Abstract: (3123 Views)
Background: Miscarriage is the spontaneous pregnancy loss before 24 wk of gestation. The incidence rate of miscarriage over the past few decades has shown steady or even growing trends. Viral intrauterine infections are one of the probable etiological causes of miscarriage. Previous evidence have shown that human herpes viruses (HHVs) could be considered as the potential reasons for intrauterine infections and adverse pregnancy outcomes.
Objective: This case-control study aimed to detect HHV1-5 DNAs in placental tissues and assess their association with miscarriage during the first 24 wk of pregnancy in spontaneous and therapeutic abortions.
Materials and Methods: Placental tissues from 83 women with spontaneous abortions during the first and the second trimesters of pregnancy and 81 women with therapeutic abortion during the same gestational age were collected. The DNA extraction was performed by the phenol/chloroform method. A part of the DNA polymerase gene of HHVs was amplified with multiplex nested-polymerase chain reaction. The polymerase chain reaction products were subjected to sequencing.
Results: The results showed the presence of human cytomegalovirus genome in the placenta of both spontaneous (8.4%) and therapeutic (4.9%) abortions. No statistically significant differences were found between these two groups. The other investigated viruses were not detected here.
Conclusion: In conclusion, like some other studies, no correlation was detected between the HHVs placental infections and the increased risk of spontaneous abortions. In order to find the actual role of HHVs infections in miscarriage, further investigations should be performed on a larger sample size in different areas.
Objective: This case-control study aimed to detect HHV1-5 DNAs in placental tissues and assess their association with miscarriage during the first 24 wk of pregnancy in spontaneous and therapeutic abortions.
Materials and Methods: Placental tissues from 83 women with spontaneous abortion
Results: The results showed the presence of human cytomegalovirus genome in the placenta of both spontaneous (8.4%) and therapeutic (4.9%) abortions. No statistically significant differences were found between these two groups. The other investigated viruses were not detected here.
Conclusion: In conclusion, like some other studies, no correlation was detected between the HHVs placental infections and the increased risk of spontaneous abortions. In order to find the actual role of HHVs infections in miscarriage, further investigations should be performed on a larger sample size in different areas.
Type of Study: Original Article |
Subject:
Reproductive Epidemiology
References
1. Lang K. Nuevo-Chiquero A. Trends in self-reported spontaneous abortions: 1970-2000. Demography 2012; 49: 989-1009. [DOI:10.1007/s13524-012-0113-0] [PMID] [PMCID]
2. Nigro G, Mazzocco M, Mattia E, Di Renzo GC, Carta G, Anceschi MM. Role of the infections in recurrent spontaneous abortion. J Matern Fetal Neonatal Med 2011; 24: 983-989. [DOI:10.3109/14767058.2010.547963] [PMID]
3. El Hachem H, Crepaux V, May-Panloup P, Descamps P, Legendre G, Bouet PE. Recurrent pregnancy loss: current perspectives. Int J Womens Health, 2017; 9: 331-345. [DOI:10.2147/IJWH.S100817] [PMID] [PMCID]
4. Giakoumelou S, Wheelhouse N, Cuschieri K, Entrican G, Howie SE, Horne AW. The role of infection in miscarriage. Hum Reprod Update 2016; 22: 116-133. [DOI:10.1093/humupd/dmv041] [PMID] [PMCID]
5. Hamilton ST, Scott G, Naing Z, Iwasenko J, Hall B, Graf N, et al. Human cytomegalovirus-induces cytokine changes in the placenta with implications for adverse pregnancy outcomes. PloS One 2012; 7: e52899. [DOI:10.1371/journal.pone.0052899] [PMID] [PMCID]
6. Finger-Jardim F, Teixeira LO, de Oliveira GR, Barral MF, da Hora VP, Gonçalves CV, et al. Herpes simplex virus: prevalence in placental tissue and incidence in neonatal cord blood samples. J Med Virol 2014; 86: 519-524. [DOI:10.1002/jmv.23817] [PMID]
7. Christian LM, Iams JD, Porter K, Glaser R. Epstein-Barr virus reactivation during pregnancy and postpartum: effects of race and racial discrimination. Brain Behav Immun 2012; 26: 1280-1287.
https://doi.org/10.1016/j.bbi.2012.08.006 [DOI:10.1016/j.bbi.2012.07.032] [PMID] [PMCID]
8. Britt WJ. Maternal immunity and the natural history of congenital human cytomegalovirus infection. Viruses 2018; 10: 405-422. [DOI:10.3390/v10080405] [PMID] [PMCID]
9. Tabata T, Petitt M, Fang-Hoover J, Rivera J, Nozawa N, Shiboski S, et al. Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model. Am J Pathol 2012; 181: 1540-1559. [DOI:10.1016/j.ajpath.2012.08.003] [PMID] [PMCID]
10. Arora N, Sadovsky Y, Dermody TS, Coyne CB. Microbial vertical transmission during human pregnancy. Cell Host Microbe 2017; 21: 561-567. [DOI:10.1016/j.chom.2017.04.007] [PMID] [PMCID]
11. Zydek M, Petitt M, Fang-Hoover J, Adler B, Kauvar LM, Pereira L, et al. HCMV infection of human trophoblast progenitor cells of the placenta is neutralized by a human monoclonal antibody to glycoprotein B and not by antibodies to the pentamer complex. Viruses 2014; 6: 1346-1364. [DOI:10.3390/v6031346] [PMID] [PMCID]
12. Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, et al. Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta. J Virol 2015; 89: 5134-5147. [DOI:10.1128/JVI.03674-14] [PMID] [PMCID]
13. Adams Waldorf KM, McAdams RM. Influence of infection during pregnancy on fetal development. Reproduction 2013; 146: R151-R162. [DOI:10.1530/REP-13-0232] [PMID] [PMCID]
14. Yan XC, Wang JH, Wang B, Huang LL, Zhou LQ, Zhu B, et al. Study of human cytomegalovirus replication in body fluids, placental infection, and miscarriage during the first trimester of pregnancy. J Med Virol 2015; 87: 1046-1053. [DOI:10.1002/jmv.24158] [PMID]
15. Straface G, Selmin A, Zanardo V, De Santis M, Ercoli A, Scambia G. Herpes simplex virus infection in pregnancy. Infectious Diseases in Obstetrics and Gynecology 2012; 2012: 1-6. [DOI:10.1155/2012/385697] [PMID] [PMCID]
16. Kim Y, Kim HS, Park JS, Kim CJ, Kim WH. Identification of Epstein-Barr Virus in the human placenta and its pathologic characteristics. J Korean Med Sci 2017; 32: 1959-1966. [DOI:10.3346/jkms.2017.32.12.1959] [PMID] [PMCID]
17. Tomai XH. Stillbirth following severe symmetric fetal growth restriction due to reactivation of Epstein-Barr virus infection in pregnancy. J Obstet Gynaecol Res 2011; 37: 1877-1882. [DOI:10.1111/j.1447-0756.2011.01662.x] [PMID]
18. Lamont RF, Sobel JD, Carrington D, Mazaki‐Tovi S, Kusanovic JP, Vaisbuch E, et al. Varicella‐zoster virus (chickenpox) infection in pregnancy. BJOG 2011; 118: 1155-1162. [DOI:10.1111/j.1471-0528.2011.02983.x] [PMID] [PMCID]
19. Shafiei-Jandaghi NZ, Yavarian J, Faghihloo E, Ghavami N, Ghalejoogh ZY, Kiani SJ, et al. Prevalence of adeno-associated virus and human papillomavirus DNA in Iranian women with and without cervical cancer. Pathol Res Pract 2017; 213: 457-460. [DOI:10.1016/j.prp.2017.02.010] [PMID]
20. Tafreshi NK, Sadeghizadeh M, Amini-Bavil-Olyaee S, Ahadi AM, Jahanzad I, Roostaee MH. Development of a multiplex nested consensus PCR for detection and identification of major human herpesviruses in CNS infections. J Clin Virol 2005; 32: 318-324. [DOI:10.1016/j.jcv.2004.05.018] [PMID]
21. Al-Buhtori M, Moore L, Benbow EW, Cooper RJ. Viral detection in hydrops fetalis, spontaneous abortion, and unexplained fetal death in utero. J Med Virol 2011; 83: 679-684. [DOI:10.1002/jmv.22007] [PMID]
22. Gao YL, Gao Z, He M, Liao P. Infection status of human parvovirus B19, cytomegalovirus and herpes simplex Virus-1/2 in women with first-trimester spontaneous abortions in Chongqing, China. Virol J 2018; 15: 74-81. [DOI:10.1186/s12985-018-0988-5] [PMID] [PMCID]
23. Sherkat R, Meidani M, Zarabian H, Rezaei A, Gholamrezaei A. Seropositivity of cytomegalovirus in patients with recurrent pregnancy loss. J Res Med Sci 2014; 19 (Suppl.): S22-S25.
24. Bagheri josheghani S, Moniri R, Baghbani Taheri F, Sadat S, Heidarrzadeh Z. Prevalence of serum antibodies to TORCH infection in the first trimester of the pregnancy in Kashan, Iran. Iranian Journal of Neonatology 2015; 6: 8-12.
25. Shaiegan M, Rasouli M, Zadsar M, Zolfaghari S. Meta-analysis of cytomegalovirus seroprevalence in volunteer blood donors and healthy subjects in Iran from 1992 to 2013. Iran J Basic Med Sci 2015; 18: 627-634.
26. Delfan-Beiranvand M, Sheikhian A, Birjandi M, Fazeli M. Seroprevalence of cytomegalovirus infection in pregnant women referred to Health Care Center of Khorramabad. Iranian Journal of Virology 2011; 5: 11-16. [DOI:10.21859/isv.5.4.11]
27. Nagamori T, Koyano S, Inoue N, Yamada H, Oshima M, Minematsu T, et al. Single cytomegalovirus strain associated with fetal loss and then congenital infection of a subsequent child born to the same mother. J Clin Virol 2010; 49: 134-136. [DOI:10.1016/j.jcv.2010.06.021] [PMID]
28. Haeri S, Baker AM, Boggess KA. Prevalence of epstein-barr virus reactivation in pregnancy. Am J Perinatol 2010; 27: 715-719. [DOI:10.1055/s-0030-1253098] [PMID]
29. Sanchez MA, Bello-Munoz JC, Cebrecos I, Sanz TH, Martinez JS, Moratonas EC, et al. The prevalence of congenital varicella syndrome after a maternal infection, but before 20 weeks of pregnancy: a prospective cohort study. J Matern Fetal Neonatal Med 2011; 24: 341-347. [DOI:10.3109/14767058.2010.497567] [PMID]
30. Borhani MS, Hoseini SM, Chamani M, Bagheri R, Kamali K, Aarabi M, et al. PCR Detection of Herpes Simplex Virus in Human Placenta and Aborted Materials in Patients with Spontaneous Abortion. Arch Clin Infect Dis 2012; 6: 17-20.
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