Volume 7, Issue 1 (7-2009)                   IJRM 2009, 7(1): 23-0 | Back to browse issues page

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Ogbuewu I P, Okoli I C, Uwaezuoke Iloeje M. Semen quality characteristics, reaction time, testis weight and seminiferous tubule diameter of buck rabbits fed neem (Azadirachta indica A. Juss) leaf meal based diets. IJRM 2009; 7 (1) :23-0
URL: http://ijrm.ssu.ac.ir/article-1-138-en.html
1- Department of Animal Science and Technology, Animal Physiology Laboratory, Federal University of Technology, Owerri, P.M.B.1526, Owerri, Nigeria
Abstract:   (2426 Views)
Background: To ascertain the effects of tropical leaf meals on semen production and semen quality.
Objective: This study was conducted with the main objective of investigating the effect of neem leaf meal on physiological responses of rabbit bucks fed graded levels of neem leaf (Azadirachta indica A. Juss) meal.
Materials and Methods: The varying levels of neem leaf meal (NLM) in the different experimental diets were 0, 5, 10 and 15% respectively. Four groups of nine crossbred New Zealand type rabbit bucks each, aged 7-8 months were randomly assigned to four diets containing neem leaf meal (NLM) at 0% (control) (CD0), 5% (CD1), 10% (CD2) and 15% (CD3) respectively for 16 weeks.
Results: The sperm concentration values obtained were 20.15 ×106 /ml, 18.04×106 /ml, 13.65×106 /ml, 6.46 ×106 /ml for the CD0, CD1, CD2 and CD3 groups respectively. The results obtained indicate that sperm motility were lowest (p<0.05) in the treatment groups than the control group. Total sperm per ejaculate was similar (p>0.05) between the control and those on 5–10 %NLM dietary groups however, the value for the 15%NLM group was significantly (p<0.05) lower than that of the control. Abnormal sperm percentage of the bucks fed 15% NLM was significantly (p<0.05) higher than those bucks on CD1, CD2 and CD3 groups. The seminiferous tubule diameters were significantly smaller in the 15% NLM (203µm) than the other 3 dietary groups. All the other variables measured including semen volume, weight of testis and reaction time did not differ (p>0.05) among the experimental group.
Conclusion: The results of the study indicate that the inclusion of neem leaf meal up to 15% in the ration of matured rabbit bucks could cause mild depressive effect on the spermatogenesis, semen quality and seminiferous tubule diameter.
Keywords: Rabbits, Neem, leaf, Histology, Semen.
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Type of Study: Original Article |

References
1. Gbadamosi AJ, Egbunike GN. Studies on cock semen (1) Effects of frequency of ejaculation and breed on physical characteristics. Trop J Anim Sci 1999; 1: 157-164.
2. Togun VA, Egbunike, GN. Seasonal variations in the sperm production characteristics of Zebu (white Fulani) cattle genitalia in the humid tropical environment. Middle-East J Sci Res 2006; 1:87-95.
3. Esonu BO, Opara MN, Okoli IC, Obikaonu HO, Udedibie C , Iheshiulor OOM. Physiological responses of laying birds to Neem (Azadirachta indica) leaf meal based diets, body weight, organ characteristics and hematology. Online J. Hlth. Allied Sci 2006
4. Herbert U, Ozoje MO, Adejumo DO. Effect of Leucaena and Gliricidia leaf meals on the seminal characteristics, testis weights and seminiferous tubule diameters of rabbits. Anim Res 2005; 54:173-178. [DOI:10.1051/animres:2005017]
5. Bawa GS, Orumuyin M, Agbaji AS, Ladan Z, Okekeifi, UO. Effects of different methods of processing Neem seeds on performance of young growing rabbits. Pak. J of Nutr 2006; 6: 212-216.
6. Herbert. Growth and reproductive characteristics of rabbits fed on Leucaena (Leucaena leucocephala) and (Gliricidia sepium) foliage 1992. PhD Thesis.
7. Herbert U, Adejumo DO. Construction and evaluation of Artificial Vagina for collecting rabbit semen. Delta Agric 1995; 2: 99-108.
8. Chibundu UC. Response of pre-pubertal bucks to administration of estradiol β. Project Report, Federal University of Technology, Owerri 2005; pp.30.
9. Majumdar NN. A textbook of Histology, Vikas Publishing House, PVT Ltd. India 1980; 57-58.
10. Steel RG, Torrie JH. Principles and Procedures of Statistics. An Approach. 2nd ed. McGraw-Hill Book Co. Inc New York, 1980; 85-87.
11. SAS. Institute Inc. SAS Technical Report Package 234 SAS/STAT Software. The GEMOD Procedure. Release 6.09.SAS Institutes Inc. Cary, NC. USA 2000.
12. Kasturi M, Manivaannam B, Nazeer AR, Parveen, DS, Pathan KM. Changes in epididymis structure and functions of albino rats treated with Neem leaves. Indian J Exp Biol 1995; 33:725-729.
13. Raghav KM, Shio KS. Effects of aqueous leaf extract of Neem on the reproductive organs in male mice. Indian J Exp Biol 2005; 43:1093-1031.
14. Aladakatti RH, Ahmed RN. Azadirachta indica induced changes in spermatogenic pattern in albino rats. J Nat Remed 2006; 6: 62-67.
15. Alvarez-Gomez AM, Cardona-Maya WD, Castro Alvarez JF, Silva J, Cadavid A. Colombia plants with spermicidal activity, new options in anti-conception: brief review 2007; 3:101-107.
16. Upadhyay SN, Dhawan S, Talwar GP. Anti-fertility effects of Neem oil in male rats by single intra-vas administration; an alternate approach to vasectomy. McAdams Health International 2008; pp.350.
17. Rahman MH. Pathological effect of feeding Leucaena leucocephala to rats. Leuc Res Rural DevRepts 1989; 9: 23-30.
18. Rahman MH, Wahab MA, Ahmed A, Iyeb A. The effect of feeding Leucaena leucocephala to Tilapia nilotica, Leuc Res Repts 1988; 9: 35-36.
19. Zemjanis R. Collection and evaluation of semen. In: Diagnostic and therapeutic techniques in animal reproduction. The Williams and Wilkins Co. Baltimore, 1977; 242.
20. Oyeyemi MO, Okediran BS. Testicular parameters and sperm morphology of chinchilla rabbits fed with different planes of soybean meal. Int J Morphol 2007; 25:139-144. [DOI:10.4067/S0717-95022007000100021]
21. Khan PK, Awasthy KS. Cytogenic toxicity of Neem. Food and Chemical Toxicology 2003; 41: 1325-1328. [DOI:10.1016/S0278-6915(03)00123-6]
22. Omarkar P, Gardner MT, The T, Williams LAD, Fletcher CK. Anti-fertility effect of aqueous and steroidal extracts of Neem leaf in male Wister rats. Short communication, Phytotherapy Research 2000; 11: 168-170.
23. Kasturi M, Manivaannam B, Nazeer AR, Parveen DS, Pathan KM. Effects of Neem leave on seminal vesicles and ventral prostate in albino rats. Indian J Physiol Pharmacol 1997; 41: 234-240.
24. Khallare MA, Shrivastav K. Spermatocidal activity of Neem leaf extract. Contraception 2003; 68: 225-229. [DOI:10.1016/S0010-7824(03)00165-3]
25. Andersen AG, Jensen TK, Carlsen E, Jorgensen N, Andersson AM, Krarup T, et al. Frequency of suboptimal semen quality in an unselected population of young men. Hum Reprod 2000; 15: 366 -372. [DOI:10.1093/humrep/15.2.366]
26. Behre HM, Yeuing CH, Holstein AF, Weinbauer GF, Gassner P, Nieschlag E. Diagnosis of male infertility and hypogonadism. In: Nieschlag E, Behre HM, eds. Andrology, Male Reproductive Health and Dysfunction. Berlin: Springer, 2000: 92.
27. MacLeod J, Wang Y. Male fertility potential in terms of semen quality: a review of the past, a study of the present. Fertil Steril 1979; 31: 103 -116. [DOI:10.1016/S0015-0282(16)43808-2]
28. Morris DL, Smith MF, Parrish W, William, JD, Wilbank JN. The effect of scrotal circumference, libido and semen quality on fertility of American Brahaman and Santa Certudies Bull. Proc. Animal management of the Society for Theriogenology, Oklahoma City 1988; 5:93-97.
29. Perry G, Petterson D. Determining reproductive fertility in herd bulls. University of Missouri Agriculture publication 2001; 2011:1-8.
30. Gupta AK, Mohanty TK. Testicular biometry and semen quality in Karan Fries bulls. Ind J Diary Sci 2003; 56: 317-319.
31. Oyeyemi MO, Oke A, Olusola C, Ajala O, Oluwatoyin O, Idehen CO. Differences in testicular parameters and morphological characteristics of spermatozoa as related to age of West African Dwarf bucks. Tropical Journal of Animal Science 2002; 5: 99-107.
32. Galmessa U, Raina VS, Mohanty TK, Gupta AK. Seminal attributes related to age and scrotal circumference in diary bulls. Ind J Dairy Sci. 2003; 56: 376-379.
33. Britto IFC, Silva AEDF, Unainian MM. Sexual development in early and late maturing Bos indicus and Bos indicus × Bos Taurus crossbred bulls in Brazil. Theriogenology, Elsevier Science Inc. New York, USA. 2004; 62:1177-2179.
34. Morton D. The use of rabbits in male reproductive toxicology. Environmental Health Perspective 1988; 77: 5-9. [DOI:10.1289/ehp.88775]
35. Berndtson WE, Igboeli G, Pickett BW. Relationship between absolute numbers of sertoli cells to testicular size and spermatogenesis in young beef bulls. J Anim Sci 1987; 64: 241-246. [DOI:10.2527/jas1987.641241x]
36. Anonymous. Divine Guide; Complete Herbal Guide for Everyone 2007; pp3.
37. Anjah RJ, Nazeer AR, Pathan KM, Manivannan B. Effects of Neem leave on testis and its recovering in albino rats. Indian J Exp Bio 1996; 34: 1091-1094.

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