Volume 7, Issue 2 (7-2009)                   IJRM 2009, 7(2): 91-95 | Back to browse issues page

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Roshangar L, Soleimani Rad J, Nikpoo P, Sayyah Melli M. Effect of oxytocin injection on folliculogenesis, ovulation and endometrial growth in mice. IJRM 2009; 7 (2) :91-95
URL: http://ijrm.ir/article-1-144-en.html
Effect of oxytocin injection on folliculogenesis, ovulation and endometrial growth in mice
Leila Roshangar1 , Jafar Soleimani Rad * 2, Parisa Nikpoo3 , Manijeh Sayyah Melli4
1- Department of Embryology and Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Embryology and Histology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran , Soleimanirj@yahoo.com
3- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4- Gynecologist, Tabriz University of Medical Sciences, Alzahra Hospital, Tabriz, Iran
Abstract:   (2566 Views)
Background: Induction of ovulation in ART is necessary for superovulation and the side effects of superovulatory drugs are debated. Oxytocin as a natural hormone, have receptors and is synthesized by several reproductive organs. Preovulatory presence of oxytocin receptor mRNAs in granulosa cells indicating a role for oxytocin in follicular development.
Objective: The aim of the present study was to investigate the effect of exogenous oxytocin injection on folliculogenesis, ovulation and endometrial growth in mice.
Materials and Methods: Forty adult female mice were divided into two groups as control and experimental. The mice at their sterous cycle received 1 IU/gr oxytocin, in experimental, and the same volume of solvent in control groups. Half of the mice in each group are sacrificed at 24 hours post injection and the other half, 48 hours after the injection. Ovarian samples fixed in 10% formalin, embedded in paraffin and sections were stained with H and E and studied using stereological techniques. The data were analyzed with Man– Whitney test.
Results: Microscopic examination revealed that the number and morphological features of follicles at different stages were similar at 24 and 48 hours post injection in both groups. The volumes of the ovaries were similar in both groups at 24 hour. However, at 48 hour, the volume of ovaries, corpora lutea and endometrial thickness, in experimental group, were significantly higher than those in control group (p< 0.05).
Conclusion: According to the increased volume of corpus luteum in the experimental group, it is concluded that oxytocin injection has a stimulatory effect on induction of ovulation.   
Keywords: Oxytocin, Folliculogenesis, Ovulation, Endometrium, Mouse.
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Type of Study: Original Article |
References
1. Opsahl MS, Robins ED, O'Connor DM, Scott RT, Fritz MA. Characteristics of gonadotropin response, follicular development and endometrial growth and maturation across consecutive cycles of clomiphene citrate treatment. Fertil Steril 1996; 66:533. [DOI:10.1016/S0015-0282(16)58564-1]
2. Tarlatzis BC, Kolibianakis EM. GnRH agonists vs antagonists. Best Pract Res Clin Obstet Gynaecol 2007; 21: 57-65. [DOI:10.1016/j.bpobgyn.2006.08.002]
3. Csemiczky G, Wramsly H, Johannisson E. Importance Of endometrial guality in women with tubal infertility during a natural menstrual cycle for the outcome of IVF treatment. J Assist Reprod Genet 1998; 15:55-61. [DOI:10.1007/BF02766825]
4. Burns PD, Mendesjr OB, Yemm RS, Clay CM, Nelson SE, Hayes SH. Cellular mechanisms by which oxytocin mediates ovine endometrial prostaglandin F2α synthesis: Role of Gi proteins and nitrogen-activated protein kinases. Biology of reproduction 2001; 65:1150-1155. [DOI:10.1095/biolreprod65.4.1150]
5. Khan-Dawood FS. Immunocytochemical localization of oxytocin and neurophysin in human corpora lutea. Am J Anat 1987; 179:18-24. [DOI:10.1002/aja.1001790104]
6. Liedman R, Hansson SR, Howe D, Igidbasian S, Mcleod A, Russell RJ, et al. Reproduction hormones in plasma over the menstrual cycle in primary dysmenorrheal compared with healthy subjects. Gynecol Endocrinol 2008; 24: 508-513. [DOI:10.1080/09513590802306218]
7. Rodgers RJ, O'Shwa JD, Findlay JK, Flint APE, Sheldrick EL. Large luteal cells: the sourco of luteal oxytocin in the sheep. Endocrinology 1983; 113: 2302-2304. [DOI:10.1210/endo-113-6-2302]
8. Gerald G, Falk F. The Oxytocin Receptor System: Structure, Function, and Regulation. Physiol Rev 2001; 81: 629-683. [DOI:10.1152/physrev.2001.81.2.629]
9. Okuda K, Uenoyama Y, Fujita Y, Iga K, Sakamoto K, Kimura T. Functional oxytocin receptors in bovine granulosa cells. Biol Reprod 1997; 56: 625-631. [DOI:10.1095/biolreprod56.3.625]
10. Furuya K, Mizumoto Y, Makimura N, Mitsui C, Murakami M, Tokuka S, et al. A novel biological aspect of ovarian oxytocin: gene expression of oxytocin and oxytocin receptor in cumulus/Iuteal cells and the effect of oxytocin on embryogenesis in fertilized occytes. Adv Exp Med Biol 1995; 395: 523-528.
11. Skarzynski DJ, Ferreira-Dias G, Okuda K. Regulation of luteal function and corpus luteum regression in cow: hormonal control, immune mechanisms and intercellular communication. Reprod Domest Anim 2008; 43: suppl: 57-65. [DOI:10.1111/j.1439-0531.2008.01143.x]
12. Einspanier A, Ivell R, Hodges JK. Oxytocin: a follicular Iuteinisation factor in the marmoset monkey. Adv Exp Med Biol 1995; 395: 517-522.
13. Robinson G, Evans JJ, Catt KJ. Oxytocin stimulates LH production by the anterior pituitary gland of the rat. Endocrinology 1992; 132: 277-283. [DOI:10.1677/joe.0.1320277]
14. Evans J.J. Peptides Interact in Gonadotrophin Regulation. Arch Physiol Biochem 2002; 110:154 - 116. [DOI:10.1076/apab.110.1.154.902]
15. Mass S, Jarry H, Teichmann A, Rath W, Kuhn W, Wuttke W. Paracrine actions of oxytocin, prostaglandin F2α and estradiol within the human corpus luteum. J Clin Endocrinol Metab 1992; 74: 306-312. [DOI:10.1210/jcem.74.2.1730809]
16. Evans JJ, Reid RA, Wakeman SA, Croft LB, Benny PS. Evidence that oxytocin is a physiological component of LH regulation in non-pregnant women. Human Reproduction 2003; 18: 1428 -1431. [DOI:10.1093/humrep/deg291]
17. Hull ML, Reid RA, Evans JJ, Benny PS, Aickin DR. Pre-ovulatory oxytocin administration promotes the onset of the luteinizing hormone surge in human females. Hum Reprod 1995; 10: 2266-2269. [DOI:10.1093/oxfordjournals.humrep.a136282]
18. Einspanier A, Jarry H, Pitzel L, Holtz W, Wuttke W. Determination of secretion of estradiol, progesterone, oxytocin, and angiotensin II from tertiary follicles and freshly formed corpora lutea in freely moving sows. Endocrinology 1991; 129:3403-3409. [DOI:10.1210/endo-129-6-3403]
19. Tallam SK, Walton JS. Effects of oxytocin on follicular development and duration of the estrous cycle in heifers. Therigenology 2000; 53: 951-962. [DOI:10.1016/S0093-691X(00)00242-9]
20. King PR, Coetzer WA. The effect of treatment with a slow-releasing oxytocin preparation at the onset of oestrus on the ovulation rate of Merino ewes. J S Afr Vet Assoc 1997, 68: 16-27. [DOI:10.4102/jsava.v68i1.859]
21. Drickamer LC. Dtermination of oestrous condition in female mice is dependent upon time of day. J Reprod Fertil 1987; 79: 659-662. [DOI:10.1530/jrf.0.0790659]
22. Robinson G, Evans JJ. Oxytocin influences preovulatory follicular development and advances ovulation in rats. Acta Endocrinol (copenh) jun 1991; 125 :109 -112. [DOI:10.1530/acta.0.1250109]
23. Wramsby H, Fredga K, Liedholm P. Chromosome analysis of human oocytes from preovulatory follicles in stimulated cycles. New Engl J Med 1987; 316: 121-124. [DOI:10.1056/NEJM198701153160301]
24. Homburg R, Armar NA, Eshel A, Adam J, Jacobs HS. Influence of serum luteinising hormone concentrations on ovulation, conception, and early pregnancy loss in polycystic ovary syndrome. Br Med J 1988; 297: 1024-1026. [DOI:10.1136/bmj.297.6655.1024]
25. Okuda K, Uenoyama Y, Fujita Y, Iga K, Sakamoto K, Kimura T. Functional oxytocin receptors in bovine granulosa cells. Biol Reprod 1997; 56: 625-631. [DOI:10.1095/biolreprod56.3.625]
26. Gimpl G, Fahrenholz F. The oxytocin receptor system: structure, function, and regulation. Physiol Rev 2001; 81: 629-683. [DOI:10.1152/physrev.2001.81.2.629]
27. Copland JA, Zlatnik MG, Ives KL, Soloff MS. Oxytocin receptor regulation and action in a human granulosa-lutein cell line. Biol Reprod 2002; 66: 1230-1236. [DOI:10.1095/biolreprod66.5.1230]
28. Amico JA, Seif SM, Robinson AG. Elevation of oxytocin and oxytocin-associated neurophysin in the plasma of normal woman during midcycle. J Clin Endocrinol Metab 1981; 53: 1229-1232. [DOI:10.1210/jcem-53-6-1229]
29. Sayyah Melli M, Tagavi S, Alizadeh M, Ghojazadeh M, Kazemi Sheshvan M. Comparison the effect of oxytocin and human chorionic gonadotropin on ovulation. J Med Sci 2007; 7: 1126- 1134. [DOI:10.3923/jms.2007.1126.1134]
30. Aladin-Chandrasekher Y, Fortune JE. Effects of oxytocin on steroidogenesis by bovine theca and granulosa cells in vitro. Endocrinology 1990; 127: 926-933. [DOI:10.1210/endo-127-2-926]
31. Mass S, Jarry H, Teichmann A, Rath W, Kuhn W, Wuttke W. Paracrine actions of oxytocin, prostaglandin F2 alpha, and estradiol within the human corpus luteum. Jornal of clinical Endocrinol Metaboli 1992; 74, 306-312. [DOI:10.1210/jcem.74.2.1730809]
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