شنبه 8 دی 1403
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دوره 7، شماره 2 - ( 4-1388 )
جلد 7 شماره 2 صفحات 64-59 |
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Fattahi E, Parivar K, Jorsaraei S G A, moghadamnia A A. The effects of diazinon on testosterone, FSH and LH levels and testicular tissue in mice. IJRM 2009; 7 (2) :59-64
URL: http://ijrm.ir/article-1-145-fa.html
URL: http://ijrm.ir/article-1-145-fa.html
The effects of diazinon on testosterone, FSH and LH levels and testicular tissue in mice. International Journal of Reproductive BioMedicine. 1388; 7 (2) :59-64
چکیده: (2909 مشاهده)
Background: Diazinon (DZN) is an organophosphate insecticide which is used worldwide in agriculture. The exposure to this chemical might lead to damages to the living systems.
Objective: The present study was done to investigate the effects of diazinon on the structure of testis and levels of sex hormones in adult male mice. Materials and Methods: For this experiment, the mature male mice divided into three groups; Control (no injection), sham (corn oil injection) and DZN (diazinon was administrated at dose of 30 mg / kg for 30 d five consecutive days per week). Animals were killed 35 days after the latest injection. Testes tissues sections were provided to investigate the histopathological changes. Serum testosterone, LH and FSH concentrations were measured by radioimmunoassay. Data were analyzed using of one-way ANOVA. Significance was set at p<0.05.
Results: A significant reduction was observed in diameter and weight of testes after DZN administration. Furthermore, DZN brought about significant reduction in sperm counts and spermatogenic, Leydig and Sertoli cells and a decrease in serum testosterone concentration. Histopathological examination of testes showed degenerative changes in seminiferous tubules (p<0.001). The levels of LH and FSH were increased in DZN groups compared to the control and sham groups (p<0.05).
Conclusion: DZN is a toxicant for mammals’ spermatogenic cells during the early spermatogenesis. Therefore, application of DZN should be limited to a designed program.
Objective: The present study was done to investigate the effects of diazinon on the structure of testis and levels of sex hormones in adult male mice. Materials and Methods: For this experiment, the mature male mice divided into three groups; Control (no injection), sham (corn oil injection) and DZN (diazinon was administrated at dose of 30 mg / kg for 30 d five consecutive days per week). Animals were killed 35 days after the latest injection. Testes tissues sections were provided to investigate the histopathological changes. Serum testosterone, LH and FSH concentrations were measured by radioimmunoassay. Data were analyzed using of one-way ANOVA. Significance was set at p<0.05.
Results: A significant reduction was observed in diameter and weight of testes after DZN administration. Furthermore, DZN brought about significant reduction in sperm counts and spermatogenic, Leydig and Sertoli cells and a decrease in serum testosterone concentration. Histopathological examination of testes showed degenerative changes in seminiferous tubules (p<0.001). The levels of LH and FSH were increased in DZN groups compared to the control and sham groups (p<0.05).
Conclusion: DZN is a toxicant for mammals’ spermatogenic cells during the early spermatogenesis. Therefore, application of DZN should be limited to a designed program.
نوع مطالعه: Original Article |
فهرست منابع
1. Sarabia L, Maurer I, Bustos-Obregón E. Melatonin prevents damage elicited by the organophosphorous pesticide diazinon on the mouse testis. Ecotoxicol Environ Saf 2009; 72: 938-942 [DOI:10.1016/j.ecoenv.2008.04.022]
2. Poet TS, Kousba AA, Dennison SL, Timchalk C. Physiologically base pharmacokinetic/pharmacodynamic model for the organophosphorus pesticide diazinon. Neurotoxicology 2004; 25:1013-1030. [DOI:10.1016/j.neuro.2004.03.002]
3. Sams C, Cocker J, Lennard MS. Biotransformation of chlorpyrifos and diazinon by human liver microsomes and recombinant human cytochrome P450s (CYP). Xenobiotica 2004; 34:861-873. [DOI:10.1080/00498250400017273]
4. Slotkin TA, Seidler FJ. Comparative developmental neurotoxicity of organophosphates in vivo: Transcriptional responses of pathways for brain cell development, cell signaling, cytotoxicity and neurotransmitter systems. Brain Res Bull 2007; 30: 232-274. [DOI:10.1016/j.brainresbull.2007.01.005]
5. Bustos-Obregon E, Gonzalez JR, Espinoza O. Melatonin as protective agent for the cytotoxic effects of diazinon in the spermatogenesis in the earthworm Eisenia foetida. Ital J Anat Embryol 2005; 110:159-165.
6. Pina-Guzman B, Solis-Heredia MJ, Quintanilla-Vega B. Diazinon alters sperm chromatin structure in mice by phosphorylating nuclear protamines. Toxicol Appl Pharmacol 2005; 15: 189-198. [DOI:10.1016/j.taap.2004.06.028]
7. Bonilla E, Hernández F, Cortés L, Mendoza M, Mejía J, Carrillo E, et al. Effects of the insecticides malathion and diazinon on the early oogenesis in mice in vitro. Environ Toxicol 2008; 23: 240-245. [DOI:10.1002/tox.20332]
8. Ducolomb Y, Casas E, Valdez A, González G, Altamirano-Lozano M, Betancourt M. In vitro effect of malathion and diazinon on oocytes fertilization and embryo development in porcine. Cell Biol Toxicol 2009 17. [DOI:10.1007/s10565-008-9117-3]
9. Mankame T, Hokanson R, Fudge R, Chowdhary R, Busbee D. Alteration of gene expression in human cells treated with the agricultural chemical diazinon: possible interaction in fetal development. Hum Exp Toxicol 2006; 25: 225-233. [DOI:10.1191/0960327106ht622oa]
10. Alahyary P, Poor MI, Azarbaijani FF, Nejati V. The potential toxicity of diazinon on physiological factors in male rat. Pak J Biol Sci 2008; 11:127-130. [DOI:10.3923/pjbs.2008.127.130]
11. Salazar-Arredondo E, de Jesús Solís-Heredia M, Rojas-García E, Hernández-Ochoa I, Quintanilla-Vega B. Sperm chromatin alteration and DNA damage by methyl-parathion, chlorpyrifos and diazinon and their oxon metabolites in human spermatozoa. Reprod Toxicol 2008; 25: 455-460. [DOI:10.1016/j.reprotox.2008.05.055]
12. Kolstad HA, Bonde JP, Spano M, Giwercman A, Zschiesche W, Kaae D, et al. Change in semen quality and sperm chromatin structure following occupational styrene exposure. Int Arch Occup Environ Health 1999; 7, 135-141. [DOI:10.1007/s004200050352]
13. Swan SH, Kruse RL, Liu F, Barr DB, Drobnis EZ, Redmon JB, et al. Study for Future Families Research Group. Semen quality in relation to biomarkers of pesticide exposure. Environ Health Perspect 2003; 111:1478-1484. [DOI:10.1289/ehp.6417]
14. Dahlgren JG, Takhar HS, Ruffalo CA, Zwass M. Health effects of diazinon on a family. J Toxicol Clin Toxicol 2004; 42: 579-591. [DOI:10.1081/CLT-200026979]
15. Altuntas I, Kilinc I, Orhan H, Demirel R, Koylu H, Delibas N. The effects of diazinon on lipid peroxidation and antioxidant enzymes in erythrocytes in vitro. Hum Exp Toxicol 2004; 23: 9-13. [DOI:10.1191/0960327104ht408oa]
16. Dutta HM, Meijer HJ. Sublethal effects of diazinon on the structure of the testis of bluegill, Lepomis macrochirus: a microscopic analysis. Enviromental pollution 2003; 125: 355-360. [DOI:10.1016/S0269-7491(03)00123-4]
17. Maxwell LB, Dutta HM. Diazinon-induced endocrine disruption in bluegill sunfish, Lepomis macrochirus. Ecotoxicol Environ Saf 2005; 60: 21-27. [DOI:10.1016/j.ecoenv.2003.12.015]
18. Wesseling C, Antich D, Hogstedt C, Rodríguez AC, Ahlbom A. Geographical differences of cancer incidence in Costa Rica in relation to environmental and occupational pesticide exposure. Int J Epidemiol 1999; 28: 365-374. [DOI:10.1093/ije/28.3.365]
19. Pidoux G, Gerbaud P, Tsatsaris V, Marpeau O, Ferreira F, Meduri G, et al. Biochemical characterization and modulation of LH/CG-receptor during human trophoblast differentiation. J Cell Physiol 2007; 212: 26-35. [DOI:10.1002/jcp.20995]
20. Peegel H, Towns R, Nair A, Menon KM. A novel mechanism for the modulation of luteinizing hormone receptor mRNA expression in the rat ovary. Mol Cell Endocrinol 2005; 233: 65-72. [DOI:10.1016/j.mce.2004.12.009]
21. Shan L, Hardy DO, Catterall JF, Hardy MP. Effects of luteinizing hormone (LH) and androgen on steady state levels of messenger ribonucleic acid for LH receptors, androgen receptors, and steroidogenic enzymes in rat Leydig cell progenitors in vivo. Endocrinology 1995; 136: 1686-1693. [DOI:10.1210/endo.136.4.7895679]
22. Sarkar R, Mohanakumar KP, Chowdhury M. Effects of an organophosphate pesticide, quinalphos, on the hypothalamo-pituitary-gonadal axis in adult male rats. Journal of Reproduction and Fertility 2000; 118: 29-38 [DOI:10.1530/jrf.0.1180029]
23. Nakai M, Hess RA, Netsu J, Nasu T. Deformation of the rat Sertoli cell by oral administration of carbendazim (methyl 2-benzimidazole carbamate). J Androl 1995; 16: 410-416.
24. Hess RA, Nakai M. Histopathology of the male reproductive system induced by the fungicide benomyl. Histol Histopathol 2000; 15: 207-224.
25. Boekelheide K, Schoenfeld HA. Spermatogenesis by Sisyphus: proliferating stem germ cells fail to repopulate the testis after 'irreversible' injury. Adv Exp Med Biol 2001; 500: 421-428. [DOI:10.1007/978-1-4615-0667-6_64]
26. Izumi H, Kimura E, Ota T, Shimazu S. A two-generation reproductive toxicity study of n-butylbenzene in rats. J Toxicol Sci 2005; 30: 21-38. [DOI:10.2131/jts.30.S21]
27. Ibrahim NA, El-Gamal BA. Effect of diazinon, an organophosphate insecticide, on plasma lipid constituents in experimental animals. J Biochem Mol Biol 2003; 36: 499-504. [DOI:10.5483/BMBRep.2003.36.5.499]
28. Narayana K, Prashanthi N, Nayanatara A, Bairy LK, D'Souza UJ. An organophosphate insecticide methyl parathion (o- o- dimethyl o - 4 - nitrophenyl phosphorothioate) induces cytotoxic damage and tubular atrophy in the testis despite elevated testosterone level in the rat. J Toxicol Sci 2006; 31:177-189. [DOI:10.2131/jts.31.177]
29. Recio R, Robins WA, Ocmpo-Gomez G, Borja-AburtoV. Organophosphorus pesticide exposure increase the frequeny of sperm sex null aneuploidy. Environ Health Prspect 2001; 109:1237-1240. [DOI:10.1289/ehp.011091237]
30. Cakir S, Sarikaya R. Genotoxicity testing of some organophosphate insecticides in the Drosophila wing spot test. Food Chem Toxicol 2005; 43: 443-450. [DOI:10.1016/j.fct.2004.11.010]
31. Prashanthi N, Narayana K, Nayanatara A, Chandra Kumar HH, Bairy KL, D'Souza UJ. The reproductive toxicity of the organophosphate pesticide 0, 0-dimethyl 0-4-nitrophenyl phosphorothioate (methyl parathion) in the male rat. Folia Morphol (Warsz) 2006; 65: 309-321.
32. Maitra SK, Sarkar R. Influence of methyl parathion on gametogenic and acetylcholinesterase activity in the testis of whitethroated munia (Lonchura malabarica). Arch Environ Contam Toxicol 1996; 30: 384-389 [DOI:10.1007/BF00212298]
33. Salem KA. Infectious diseases among food handlers. J Egypt Public Health Assoc 1998; 73: 563-575.
34. Hatjian BA, Mutch E, Williams FM, Blain PG, Edwards JW. Cytogenetic response without changes in peripheral cholinesterase enzymes following exposure to a sheep dip containing diazinon in vivo and in vitro. Mutat Res 2000; 427, 85-92. [DOI:10.1016/S1383-5718(00)00131-5]
35. Aluigi MG, Angelini C, Falugi C, Fossa R, Genever P, Gallus L, et al. Interaction between organophosphate compounds and cholinergic functions during development. Chem Biol Interact 2005; 157-158:305-316. [DOI:10.1016/j.cbi.2005.10.037]
36. LeBlanc G, Bain LG, Wilson VS. Pesticides:Multiple mechanisms of demasculi- nization. Mol Cell Endocrinol 1997; 126:1-5. [DOI:10.1016/S0303-7207(96)03968-8]
37. Watanabe HK, Hoskins B, Ho IK. Selective inhibitory effect of organophosphates on UDP-glucuronyl transferase activities in rat liver microsomes. Biochem Pharmacol 1986; 35:455-460. [DOI:10.1016/0006-2952(86)90219-4]
38. Civen M, Brown CB. The effect of organophosphate insecticides on adrenal corticosterone formation. Pesticide Biochemistry and Physiology 1974; 4: 254-259. [DOI:10.1016/0048-3575(74)90108-4]
39. Chattopadhyay A, Sarkar M, Biswas NM. Dose-dependent effect of copper chloride on male reproductive function in immature rats. Kathmandu Univ Med J (KUMJ) 2005; 3: 392-400.
40. Pidoux G, Gerbaud P, Tsatsaris V, Marpeau O, Ferreira F, Meduri G, et al. Biochemical characterization and modulation of LH/CG-receptor during human trophoblast differentiation. J Cell Physiol 2007; 212: 26-35. [DOI:10.1002/jcp.20995]
41. Tamura H, Maness SC, Reischmann K, Dorman DC, Gray LE, Gaido KW. Androgen receptor antagonism by the organophosphate insecticide fenitrothion. Toxicol Sci 2001; 60: 56-62. [DOI:10.1093/toxsci/60.1.56]
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