Volume 19, Issue 3 (March 2021)                   IJRM 2021, 19(3): 217-226 | Back to browse issues page

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Argyraki M, Katafigiotis S, Vavilis T, Papadopoulou Z, Tzimagiorgis G, Haidich A, et al . Influence of conception and delivery mode on stress response marker Oct4B1 and imprinted gene expression related to embryo development: A cohort study. IJRM 2021; 19 (3) :217-226
URL: http://ijrm.ir/article-1-1483-en.html
Influence of conception and delivery mode on stress response marker Oct4B1 and imprinted gene expression related to embryo development: A cohort study
Maria Argyraki * 1, Socrates Katafigiotis2 , Theofanis Vavilis3 , Zoe Papadopoulou4 , Giorgos Tzimagiorgis5 , Anna-Bettina Haidich6 , Katerina Chatzimeletiou7 , Grigoris Grimbizis7 , Basil Tarlatzis7 , Maria Syrrou7 , Alexandros Lambropoulos2
1- Laboratory of Genetics, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece. , argyrakimar@gmail.com
2- Laboratory of Genetics, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece.
3- Laboratory of Biology and Genetics, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
4- Laboratory of Biology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Ioannina, Greece.
5- Laboratory of Biological Chemistry, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
6- Department of Hygiene and Epidemiology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
7- Unit for Human Reproduction, 1st Department of Obstetrics and Gynecology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki, Greece.
Abstract:   (1863 Views)
Background: Recent scientific data support that the mode of conception and delivery may influence epigenetic regulation and therefore embryo development. Octamer-binding transcription factor 4-B1 (OCT4B1), a novel variant of OCT4 with yet unknown biological function, is suggested to have a potential role in mediating cellular stress response. Furthermore, Insulin-like Growth Factor 2 (IGF2), Mesoderm-specific Transcript (MEST) and paternally expressed gene 10 (PEG10) are genes known as imprinted and are regulated via means of epigenetic regulation. The influence of delivery mode and conception on epigenetic regulation is an active research field.
Objective: Our aim was to correlate the expression level of Oct4B1 and the expression and methylation level of IGF2, MEST, and PEG10 imprinted genes with the mode of delivery and conception in the umbilical cord blood of newborns.
Materials and Methods: Samples of umbilical cord blood from infants born after vaginal delivery, caesarean section (CS) with the infant in cephalic position and CS due to breech position were examined. Furthermore, the investigation included infants conceived through means of assisted reproductive technology.
Results: No statistically significant differences were found in mRNA expression levels between different modes of conception and delivery (p = 0.96). Oct4B1, IGF2, MEST, and PEG10 expression levels do not seem to be significantly affected by different modes of conception and delivery.
Conclusion: These results indicate that the expression and methylation patterns of Oct4B1, IGF2, MEST and PEG10 in umbilical cord blood are not affected by the conception and delivery mode.
Keywords: Conception, Fertilization in vitro, Genomic imprinting, Fetalblood.
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Type of Study: Short Research Reports | Subject: Reproductive Biology
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