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Volume 18, Issue 8 (August 2020)
IJRM 2020, 18(8): 637-650 |
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Ashkar F, Eftekhari M H, Tanideh N, Koohpeyma F, Mokhtari M, Irajie K et al . Effect of hydroalcoholic extract of Berberis integerrima and resveratrol on ovarian morphology and biochemical parameters in Letrozole-induced polycystic ovary syndrome rat model: An experimental study. IJRM 2020; 18 (8) :637-650
URL: http://ijrm.ir/article-1-1486-en.html
URL: http://ijrm.ir/article-1-1486-en.html
Fatemeh Ashkar1 
, Mohammad Hassan Eftekhari1 , Nader Tanideh * 2, Farhad Koohpeyma3 , Maral Mokhtari4 , Kambyz Irajie5 , Aida Iraji6
, Mohammad Hassan Eftekhari1 , Nader Tanideh * 2, Farhad Koohpeyma3 , Maral Mokhtari4 , Kambyz Irajie5 , Aida Iraji6
1- Department of Clinical Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2- Stem cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ,tanidehn@gmail.com
3- Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5- Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
6- Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
2- Stem cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ,
3- Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4- Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5- Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
6- Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract: (2705 Views)
Background: Resveratrol and Berberis integerrima (B. integerrima) are known to be natural antioxidants and regulators of human metabolism. However, the effects of resveratrol and B. integerrima on the ovarian morphology in polycystic ovary syndrome (PCOS) are not obvious.
Objective: This study aimed to determine the effect of the hydroalcoholic extract of B. integerrima in combination with resveratrol on some biochemical parameters and ovarian morphology in the letrozole-induced PCOS rat.
Materials and Methods: Seventy adult female Sprague-Dawley rats aged 10-12 weeks weighing 200 ± 20 gr were randomly divided into seven groups (n = 10/each). Group I): normal; Group II): vehicle; Group III): letrozole-induced PCOS 1 mg/kg letrozole orally, rats receiving 1 cc normal saline orally; Group IV): PCOS + receiving 150 mg/kg metformin orally; Group V): PCOS + receiving 20 mg/kg resveratrol orally; Group VI): PCOS + 3 gr/kg barberry orally; and Group VII): PCOS + receiving 3 gr/kg barberry and 20 mg/kg resveratrol orally. All animals were followed-up for 63 days. The biochemical parameters and histological assessments of ovaries were performed.
Results: Resveratrol alone and/or in combination with B. integerrima treatment in rats led to a significant decrease in low-density lipoprotein, triglyceride, malondialdehyde , and tumor necrosis factor-alpha concentrations (p = 0.02). The groups IV, V, VI, and VII showed a decrease in insulin resistance and an increase in the superoxide dismutase, total antioxidant capacity, and high-density lipoprotein (p =0.01). No significant difference was observed between the level of serum glucose in the treatment groups. Number of cystic follicles had a significant decrease in barberry, resveratrol, and their combination groups (p < 0.001).
Conclusion: Resveratrol, B. integerrima, and their combination as natural products with fewer side effects might be effective as an alternative medicine in treatment of PCOS.
Objective: This study aimed to determine the effect of the hydroalcoholic extract of B. integerrima in combination with resveratrol on some biochemical parameters and ovarian morphology in the letrozole-induced PCOS rat.
Materials and Methods: Seventy adult female Sprague-Dawley rats aged 10-12 weeks weighing 200 ± 20 gr were randomly divided into seven groups (n = 10/each). Group I): normal; Group II): vehicle; Group III): letrozole-induced PCOS 1 mg/kg letrozole orally, rats receiving 1 cc normal saline orally; Group IV): PCOS + receiving 150 mg/kg metformin orally; Group V): PCOS + receiving 20 mg/kg resveratrol orally; Group VI): PCOS + 3 gr/kg barberry orally; and Group VII): PCOS + receiving 3 gr/kg barberry and 20 mg/kg resveratrol orally. All animals were followed-up for 63 days. The biochemical parameters and histological assessments of ovaries were performed.
Results: Resveratrol alone and/or in combination with B. integerrima treatment in rats led to a significant decrease in low-density lipoprotein, triglyceride, malondialdehyde , and tumor necrosis factor-alpha concentrations (p = 0.02). The groups IV, V, VI, and VII showed a decrease in insulin resistance and an increase in the superoxide dismutase, total antioxidant capacity, and high-density lipoprotein (p =0.01). No significant difference was observed between the level of serum glucose in the treatment groups. Number of cystic follicles had a significant decrease in barberry, resveratrol, and their combination groups (p < 0.001).
Conclusion: Resveratrol, B. integerrima, and their combination as natural products with fewer side effects might be effective as an alternative medicine in treatment of PCOS.
Type of Study: Original Article |
Subject:
Fertility & Infertility
References
1. Zahiri Z, Sharami SH, Milani F, Mohammadi F, Kazemnejad E, Ebrahimi H, et al. Metabolic syndrome in patients with polycystic ovary syndrome in Iran. Int J Fertil Steril 2016; 9: 490-496.
2. Ghowsi M, Khazali H, Sisakhtnezhad S. The effect of resveratrol on oxidative stress in the liver and serum of a rat model of polycystic ovary syndrome: An experimental study. Int J Reprod Biomed 2018; 16: 149-158. [DOI:10.29252/ijrm.16.3.149]
3. Bailey B, Phinney S, Volek J. Polycystic Ovarian Syndrome, Insulin Resistance and Inflammation. Retrieved (2019, February 2) from https://blog virtahealth com/pcos-polycystic-ovarian-syndrome. 2019.
4. Ziaee A, Oveisi S, Abedini A, Hashemipour S, Karimzadeh T, Ghorbani A. Effect of metformin and pioglitazone treatment on cardiovascular risk profile in polycystic ovary syndrome. Acta Med Indones 2012; 44: 16-22.
5. Kwon CY, Lee B, Park KS. Oriental herbal medicine and moxibustion for polycystic ovary syndrome: A meta-analysis. Medicine 2018; 97: e12942-e12954. [DOI:10.1097/MD.0000000000012942] [PMID] [PMCID]
6. Minaiyan M, Ghannadi A, Mahzouni P, Jaffari-Shirazi E. Comparative study of Berberis vulgaris fruit extract and berberine chloride effects on acetic acid-induced colitis in rats. Iran J Pharm Res 2011; 10: 97-104.
7. El-Wahab AEA, Ghareeb DA, Sarhan EEM, Abu-Serie MM, El Demellawy MA. In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects. BMC Complement Altern Med 2013; 13: 218-229. [DOI:10.1186/1472-6882-13-218] [PMID] [PMCID]
8. Lazavi F, Mirmiran P, Sohrab G, Nikpayam O, Angoorani P, Hedayati M. The barberry juice effects on metabolic factors and oxidative stress in patients with type 2 diabetes: a randomized clinical trial. Complement Ther Clin Pract 2018; 31: 170-174. [DOI:10.1016/j.ctcp.2018.01.009] [PMID]
9. Kumar A, Chopra K, Mukherjee M, Pottabathini R, Dhull DK. Current knowledge and pharmacological profile of berberine: An update. Eur J Pharmacol 2015; 761: 288-297. [DOI:10.1016/j.ejphar.2015.05.068] [PMID]
10. Tomé-Carneiro J, Larrosa M, González-Sarrías A, Tomas-Barberan FA, Teresa Garcia-Conesa M, Carlos Espin J. Resveratrol and clinical trials: the crossroad from in vitro studies to human evidence. Curr Pharm Des 2013; 19: 6064-6093. [DOI:10.2174/13816128113199990407] [PMID] [PMCID]
11. Ergenoglu M, Yildirim N, Sahingoz Yildirim AG, Yeniel O, Erbas O, Yavasoglu A, et al. Effects of resveratrol on ovarian morphology, plasma anti-mullerian hormone, IGF-1 levels, and oxidative stress parameters in a rat model of polycystic ovary syndrome. Reprod Sci 2015: 22: 942-947. [DOI:10.1177/1933719115570900] [PMID]
12. Baravalle C, Salvetti NR, Mira GA, Pezzone N, Ortega HH. Microscopic characterization of follicular structures in letrozole-induced polycystic ovarian syndrome in the rat. Arch Med Res 2006; 37: 830-839. [DOI:10.1016/j.arcmed.2006.04.006] [PMID]
13. Di Pietro M, Parborell F, Irusta G, Pascuali N, Bas D, Bianchi MS, et al. Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model. Endocrinology 2015; 156: 1453-1463. [DOI:10.1210/en.2014-1765] [PMID]
14. Tanideh N, Afaridi E, Mehrabani D, Azarpira N, Hosseinzadeh M, Amini M, et al. The healing effect of berberis vulgaris in acetic acid-induced ulcerative colitis in rat. Middle-East J Sci Res 2014; 21: 1288-1294.
15. Iraji A, Firuzi O, Khoshneviszadeh M, Nadri H, Edraki N, Miri R. Synthesis and structure-activity relationship study of multi-target triazine derivatives as innovative candidates for treatment of Alzheimer's disease. Bioorg Chem 2018; 77: 223-235. [DOI:10.1016/j.bioorg.2018.01.017] [PMID]
16. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology 1995; 28: 25-30. [DOI:10.1016/S0023-6438(95)80008-5]
17. Koohi-Hosseinabadi O, Ranjbar Z, Sepehrimanesh M, AndisheTadbir A, Poorbaghi SL, Bahranifard H, et al. Biochemical, hematological, and pathological related healing effects of Elaeagnus angustifolia hydroalcoholic extract in 5-fluorouracil-induced oral mucositis in male golden hamster. Enviro Sci Pollut Res Int 2017; 24: 24447-24453. [DOI:10.1007/s11356-017-0137-5] [PMID]
18. Tanideh N, Bahrani M, Khoshnood-Mansoorkhani MJ, Mehrabani D, Firoozi D, Koohi-Hosseinabadi O, et al. Evaluating the effect of melillotus officinalis L. Aqueous extracts on healing of acetic acid-induced ulcerative colitis in male rats. Ann Colorectal Res 2016; 4: e42856-e42862. [DOI:10.17795/acr-42856]
19. Valavi M, Mezginejad F, Haghighi F, Hemmati M, Zarban A, Rabiei Gask E. Effectiveness of aqueous and alcoholic extracts of barberry, jujube, and saffron against oxidative stress in streptozotocin-induced diabetic rats. Mod Care J 2016; 13: e11162-e11167. [DOI:10.5812/modernc.11162]
20. Fonseca ÉJNdC, Rocha TPO, Nogueira IAL, Melo JBd, Silva BLE, Lopes EJ, et al. Metabolic syndrome and insulin resistance by HOMA-IR in menopause. Int J Cardiovasc Sci 2018; 31: 201-208. [DOI:10.5935/2359-4802.20180009]
21. Ihsan I, Tehreem A, Rasool S. Significance of TNF-Alpha and insulin resistance in women with polycystic ovarian syndrome. Pakistan J Med Health Sci 2018; 12: 459-463.
22. Manneras L, Cajander S, Holmang A, Seleskovic Z, Lystig T, Lönn M, et al. A new rat model exhibiting both ovarian and metabolic characteristics of polycystic ovary syndrome. Endocrinology 2007; 148: 3781-3791. [DOI:10.1210/en.2007-0168] [PMID]
23. Walters KA, Bertoldo MJ, Handelsman DJ. Evidence from animal models on the pathogenesis of PCOS. Best Pract Res Clin Endocrinol Metab 2018; 32: 271-281. [DOI:10.1016/j.beem.2018.03.008] [PMID]
24. Noorafshan A, Ahmadi M, Mesbah SF, Karbalay-Doust S. Stereological study of the effects of letrozole and estradiol valerate treatment on the ovary of rats. Clin Exp Reprod Med 2013; 40: 115-121. [DOI:10.5653/cerm.2013.40.3.115] [PMID] [PMCID]
25. Webber LJ, Stubbs S, Stark J, Trew GH, Margara R, Hardy K, et al. Formation and early development of follicles in the polycystic ovary. Lancet 2003; 362: 1017-1021. [DOI:10.1016/S0140-6736(03)14410-8]
26. Charehsaz M, Sipahi H, Celep E, Üstündağ A, Ülker ÖC, Duydu Y, et al. The fruit extract of Berberis crataegina DC: exerts potent antioxidant activity and protects DNA integrity. DARU J Pharm Sci 2015; 23: 24-30. [DOI:10.1186/s40199-015-0108-7] [PMID] [PMCID]
27. Sabahi Z, Farmani F, Soltani F, Moein M. DNA protection, antioxidant and xanthine oxidase inhibition activities of polyphenol-enriched fraction of Berberis integerrima Bunge fruits. Iran J Basic Med Sci 2018; 21: 411-416.
28. Sharifi F, Poorakbar L. The survey of antioxidant properties of phenolic compounds in fresh and dry hybrid Barberry fruits (Berberis integerrima× vulgaris). Cumhuriyet Üniversitesi Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi 2015; 36: 1609-1617.
29. Maharjan R, Nagar PS, Nampoothiri L. Effect of Aloe barbadensis Mill. formulation on Letrozole induced polycystic ovarian syndrome rat model. J Ayurveda Integr Med 2010; 1: 273-279. [DOI:10.4103/0975-9476.74090] [PMID] [PMCID]
30. Padalkar RK, Patil SM, Andure DV, Bhagat SS, Raut AM. Study of hormone and lipid profile in polycystic ovarian syndrome women between the age 18 to 30 years. J Pract Biochem Biophys 2017; 2: 11-15.
31. Hadi A, Arab A, Ghaedi E, Rafie N, Miraghajani M, Kafeshani M. Barberry (Berberis vulgaris L.) is a safe approach for management of lipid parameters: A systematic review and meta‐analysis of randomized controlled trials. Complement Ther Med 2019; 43: 117-124. [DOI:10.1016/j.ctim.2019.01.017] [PMID]
32. Derosa G, D'Angelo A, Bonaventura A, Bianchi L, Romano D, Maffioli P. Effects of berberine on lipid profile in subjects with low cardiovascular risk. Expert Opin Biol Ther 2013; 13: 475-482. [DOI:10.1517/14712598.2013.776037] [PMID]
33. Shamsa F, Ahmadiani A, Khosrokhavar R. Antihistaminic and anticholinergic activity of barberry fruit (Berberis vulgaris) in the guinea-pig ileum. J Ethnopharmacol 1999; 64: 161-166. [DOI:10.1016/S0378-8741(98)00122-6]
34. Simental-Mendía LE, Guerrero-Romero F. Effect of resveratrol supplementation on lipid profile in subjects with dyslipidemia: A randomized double-blind, placebo-controlled trial. Nutrition 2019; 58: 7-10. [DOI:10.1016/j.nut.2018.06.015] [PMID]
35. Cho IJ, Ahn JY, Kim S, Choi MS, Ha TY. Resveratrol attenuates the expression of HMG-CoA reductase mRNA in hamsters. Biochem Biophys Res Commun 2008; 367: 190-194. [DOI:10.1016/j.bbrc.2007.12.140] [PMID]
36. Yashiro T, Nanmoku M, Shimizu M, Inoue J, Sato R. Resveratrol increases the expression and activity of the low density lipoprotein receptor in hepatocytes by the proteolytic activation of the sterol regulatory element-binding proteins. Atherosclerosis 2012; 220: 369-374. [DOI:10.1016/j.atherosclerosis.2011.11.006] [PMID]
37. Bonnefont-Rousselot D. Resveratrol and cardiovascular diseases. Nutrients 2016; 8: 250-273. [DOI:10.3390/nu8050250] [PMID] [PMCID]
38. Victor VM, Rocha M, Bañuls C, Alvarez A, de Pablo C, Sanchez-Serrano M, et al. Induction of oxidative stress and human leukocyte/endothelial cell interactions in polycystic ovary syndrome patients with insulin resistance. J Clin Endocrinol Metab 2011; 96: 3115-3122. [DOI:10.1210/jc.2011-0651] [PMID]
39. Zhu X, Liu Q, Wang M, Liang M, Yang X, Xu X, et al. Activation of Sirt1 by resveratrol inhibits TNF-alpha induced inflammation in fibroblasts. PloS One 2011; 6: e27081. [DOI:10.1371/journal.pone.0027081] [PMID] [PMCID]
40. Zhu J, Yong W, Wu X, Yu Y, Lv J, Liu C, et al. Anti-inflammatory effect of resveratrol on TNF-α-induced MCP-1 expression in adipocytes. Biochem Biophys Res Commun 2008; 369: 471-477. [DOI:10.1016/j.bbrc.2008.02.034] [PMID]
41. Sebastiano M, Eens M, Messina S, AbdElgawad H, Pineau K, Beemster GTS, et al. Resveratrol supplementation reduces oxidative stress and modulates the immune response in free‐living animals during a viral infection. Functional Ecology 2018; 32: 2509-2519. [DOI:10.1111/1365-2435.13195]
42. Imenshahidi M, Hosseinzadeh H. Berberis vulgaris and berberine: An update review. Phytother Res 2016; 30: 1745-1764. [DOI:10.1002/ptr.5693] [PMID]
43. Li Z, Geng YN, Jiang JD, Kong WJ. Antioxidant and anti-inflammatory activities of berberine in the treatment of diabetes mellitus. Evid Based Complement Alternat Med 2014; 2014: 289264-289276. [DOI:10.1155/2014/289264] [PMID] [PMCID]
44. Bergman RN, Finegood DT, Kahn SE. The evolution of β‐cell dysfunction and insulin resistance in type 2 diabetes. Eur J Clin Invest 2002; 32 (Suppl.): 35-45. [DOI:10.1046/j.1365-2362.32.s3.5.x] [PMID]
45. Firouzi S, Malekahmadi M, Ghayour-Mobarhan M, Ferns G, Rahimi HR. Barberry in the treatment of obesity and metabolic syndrome: possible mechanisms of action. Diabetes Metab Syndr Obes 2018; 11: 699-705. [DOI:10.2147/DMSO.S181572] [PMID] [PMCID]
46. Pérez-Rubio KG, González-Ortiz M, Martínez-Abundis E, Robles-Cervantes JA, Espinel-Bermúdez MC. Effect of berberine administration on metabolic syndrome, insulin sensitivity, and insulin secretion. Metab Syndr Relat Disord 2013; 11: 366-369. [DOI:10.1089/met.2012.0183] [PMID]
47. Ashraf H, Heidari R, Nejati V, Ilkhanipoor M. [Preventive effect of berberis integerrima on the serum levels of glucose and lipids in streptozotocin (stz)-induced diabetes in rats]. J Fasa Univ Med Sci 2012; 2: 148-155. (in Persian)
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