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Volume 18, Issue 8 (August 2020)
IJRM 2020, 18(8): 597-604 |
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Sadeghi S, Jalali M, Nikravesh M R, Sankian M. Effect of experimental hyperthyroidism on CatSper1 and CatSper2 genes expression in the seminiferous tubules of BALB/c mice: An experimental study. IJRM 2020; 18 (8) :597-604
URL: http://ijrm.ir/article-1-1495-en.html
URL: http://ijrm.ir/article-1-1495-en.html
1- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
2- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran. , JalaliM@mums.ac.ir
3- Immunology Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
2- Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran. , JalaliM@mums.ac.ir
3- Immunology Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran.
Abstract: (1688 Views)
Background: CATSPER 1 (Cation Channel Sperm Associated 1) and CATSPER2 channels have an important role in sperm motility. In this study, the effects of hyperthyroidism on Catsper1 and 2 genes of seminiferous tubules in mice testes were investigated.
Objective: The present study was conducted to investigate the effect of hyperthyroidism on the expression of CATSPER1 and CATSPER2 genes in the seminiferous tubules of mice.
Materials and Methods: This study was conducted on 20 BALB/C male mice divided into two groups - experimental and control. The experimental group was administered with 500 mg/l levothyroxine (L-thyroxine) liquid solution for two months for inducing hyperthyroidism, which was confirmed by radioimmunoassay. On the other hand, the control group was kept in animal houses under a normal condition. The implementation of real-time polymerase chain reaction and immunohistochemical studies was accomplished after the removal of the testes of the mice under anesthesia induced by chloroform.
Results: Results showed that there was no significant difference in CATSPER1 (p = 0.45) and CATSPER2 (p = 0.34) gene expression between groups. At the same time, the color intensity showed no significant enhancement in the hyperthyroidism group (CATSPER1 p = 0.17 and CATSPER2 p = 0.22) as compared to the control group.
Conclusion: Considering the key role of CATSPER in the molecular structure of the sperm, our findings showed that the hyperactivity of the thyroid gland has no significant effects on the function of these components. Therefore, it might be concluded that hyperthyroidism has no considerable effects on the seminiferous tubules.
Objective: The present study was conducted to investigate the effect of hyperthyroidism on the expression of CATSPER1 and CATSPER2 genes in the seminiferous tubules of mice.
Materials and Methods: This study was conducted on 20 BALB/C male mice divided into two groups - experimental and control. The experimental group was administered with 500 mg/l levothyroxine (L-thyroxine) liquid solution for two months for inducing hyperthyroidism, which was confirmed by radioimmunoassay. On the other hand, the control group was kept in animal houses under a normal condition. The implementation of real-time polymerase chain reaction and immunohistochemical studies was accomplished after the removal of the testes of the mice under anesthesia induced by chloroform.
Results: Results showed that there was no significant difference in CATSPER1 (p = 0.45) and CATSPER2 (p = 0.34) gene expression between groups. At the same time, the color intensity showed no significant enhancement in the hyperthyroidism group (CATSPER1 p = 0.17 and CATSPER2 p = 0.22) as compared to the control group.
Conclusion: Considering the key role of CATSPER in the molecular structure of the sperm, our findings showed that the hyperactivity of the thyroid gland has no significant effects on the function of these components. Therefore, it might be concluded that hyperthyroidism has no considerable effects on the seminiferous tubules.
Type of Study: Original Article |
Subject:
Reproductive Anatomy
References
1. Singh R, Hamada AJ, Agarwal A. Thyroid hormones in male reproduction and fertility. Open Reprod Sci J 2011; 3: 98-104. [DOI:10.2174/1874255601103010098]
2. Brent GA. Mechanisms of thyroid hormone action. J Clin Invest 2012; 122: 3035-3043. [DOI:10.1172/JCI60047] [PMID] [PMCID]
3. Wagner MS, Wajner SM, Maia AL. The role of thyroid hormone in testicular development and function. J Endocrinol 2008; 199: 351-365. [DOI:10.1677/JOE-08-0218] [PMID] [PMCID]
4. Ai J, Zarifkar AA, Takhshid MA, Alavi J, Moradzadeh M. The effect of thyroid activity on adult rat spermatogenesis. Iran J Vet Res 2007; 8: 155-160.
5. Souhila DS, Zohra HS, Kame A, Hadj-Bekkouche F. Effects of thyroxine treatment during lactation on the testicular function of rats across different ages. Folia Histochem Cytobiol 2013; 51: 107-114. [DOI:10.5603/FHC.2013.0017] [PMID]
6. Holsberger DR, Kiesewetter SE, Cooke PS. Regulation of neonatal Sertoli cell development by thyroid hormone receptor α1. Biol Reprod 2005; 73: 396-403. [DOI:10.1095/biolreprod.105.041426] [PMID]
7. Liu CR, Li LY, Shi F, Zang XY, Liu YM, Sun Y, et al. Effects of hyper-and hypothyroid on expression of thyroid hormone receptor mRNA in rat myocardium. J Endocrinol 2007; 195: 429-438. [DOI:10.1677/JOE-07-0253] [PMID]
8. Silveira GF, Buffon A, Bruno AN. New approaches to thyroid hormones and purinergic signaling. J Thyroid Res 2013; 2013: 434727. [DOI:10.1155/2013/434727] [PMID] [PMCID]
9. Li HG, Ding XF, Liao AH, Kong XB, Xiong CL. Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility. Mol Hum Reprod 2007; 13: 299-306. [DOI:10.1093/molehr/gam009] [PMID]
10. Park EH, Kim DR, Kim HY, Park SK, Chang MS. Panax ginseng induces the expression of CatSper genes and sperm hyperactivation. Asian J Androl 2014; 16: 845-851. [DOI:10.4103/1008-682X.129129] [PMID] [PMCID]
11. Singh AP, Rajender S. CatSper channel, sperm function and male fertility. Reprod Biomed Online 2015; 30: 28-38. [DOI:10.1016/j.rbmo.2014.09.014] [PMID]
12. Xia J, Reigada D, Mitchell CH, Ren D. CATSPER channel-mediated Ca2+ entry into mouse sperm triggers a tail-to-head propagation. Biol Reprod 2007; 77: 551-559. [DOI:10.1095/biolreprod.107.061358] [PMID]
13. Amini M, Shirinbayan P, Behnam B, Roghani M, Farhoudian A, Joghataei MT, et al. Correlation between expression of CatSper family and sperm profiles in the adult mouse testis following I ranian Kerack abuse. Andrology 2014; 2: 386-393. [DOI:10.1111/j.2047-2927.2014.00195.x] [PMID]
14. Mohammadi S, Jalali M, Nikravesh MR, Fazel A, Ebrahimzadeh A, Gholamin M, et al. Effects of Vitamin-E treatment on CatSper genes expression and sperm quality in the testis of the aging mouse. Iran J Reprod Med 2013; 11: 989-998.
15. Qi H, Moran MM, Navarro B, Chong JA, Krapivinsky G, Krapivinsky L, et al. All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility. Proc Nati Acad Sci USA 2007; 104: 1219-1223. [DOI:10.1073/pnas.0610286104] [PMID] [PMCID]
16. Loux SC, Crawford KR, Ing NH, González-Fernández L, Macías-García B, Love CC, et al. CatSper and the relationship of hyperactivated motility to intracellular calcium and pH kinetics in equine sperm. Biol Reprod 2013; 89: 123-137. [DOI:10.1095/biolreprod.113.111708] [PMID]
17. Ren D, Navarro B, Perez G, Jackson AC, Hsu S, Shi Q, et al. A sperm ion channel required for sperm motility and male fertility. Nature 2001; 413: 603-609. [DOI:10.1038/35098027] [PMID]
18. Ferreira E, Silva AE, Serakides R, Gomes AES, Cassali GD. Model of induction of thyroid dysfunctions in adult female mice. Arq Bras Med Vet Zootec 2007; 59: 1245-1249. [DOI:10.1590/S0102-09352007000500022]
19. Rijntjes E, Wientjes AT, Swarts HJ, de Rooij DG, Teerds KJ. Dietary-induced hyperthyroidism marginally affects neonatal testicular development. J Androl 2008; 29: 643-653. [DOI:10.2164/jandrol.108.005108] [PMID]
20. Tamburrino L, Marchiani S, Minetti F, Forti G, Muratori M, Baldi E. The CatSper calcium channel in human sperm: relation with motility and involvement in progesterone-induced acrosome reaction. Hum Reprod 2014; 29: 418-428. [DOI:10.1093/humrep/det454] [PMID]
21. Tamburrino L, Marchiani S, Vicini E, Muciaccia B, Cambi M, Pellegrini S, et al. Quantification of CatSper1 expression in human spermatozoa and relation to functional parameters. Hum Reprod 2015; 30: 1532-1544. [DOI:10.1093/humrep/dev103] [PMID]
22. Mohammadi S, Gholamin M, Mansouri A, Mahmoodian RS, Babazadeh B, Kebriaei SM, et al. Effect of cadmium and nickel on expression of CatSper 1 and 2 genes in mice. Toxin Rev 2018; 37: 216-222. [DOI:10.1080/15569543.2017.1350192]
23. Mohammadi S, Movahedin M, Mowla SJ. Up-regulation of CatSper genes family by selenium. Reprod Biol Endocrinol 2009; 7: 126-131. [DOI:10.1186/1477-7827-7-126] [PMID] [PMCID]
24. Mohammadi S, Jalali M, Nikravesh MR, Gholamin M, Fazel A, Ebrahimzadeh A, et al. Effects of L-carnitine treatment on expression of CatSper proteins in the aging mouse model. Eur J Exp Biol 2013; 3: 731-735.
25. Alipour F, Jalali M, Nikravesh MR, Fazel A, Sankian M, Khordad E. Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice. Acta Biol Hung 2018; 69: 244-258. [DOI:10.1556/018.68.2018.3.2] [PMID]
26. Soleimani MZ, Jalali Mashayekhi F, Mousavi Hasanzade M, Baazm M. Alteration in CatSper1 and 2 genes expression, sperm parameters and testis histology in varicocelized rats. Int J Reprod Biomed 2018; 16: 183-190. [DOI:10.29252/ijrm.16.3.183]
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