Volume 17, Issue 4 (April 2019 2019)                   IJRM 2019, 17(4): 279-286 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeghi S, Talebi A R, Shahedi A, Moein M R, Abbasi-sarcheshmeh A. Effects of different doses of tamoxifen on the sperm parameters and chromatin quality in mice: An experimental model. IJRM 2019; 17 (4) :279-286
URL: http://ijrm.ir/article-1-1496-en.html
1- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. , prof_talebi@ssu.ac.ir
3- Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract:   (8069 Views)
Background: Tamoxifen (TX) is widely used for the treatment of male factor and idiopathic infertility. It has been shown that TX induces sperm production and so improves male fertility.
Objective: This study evaluated the effects of different doses of TX on the sperm parameters and chromatin quality in mice.
Materials and Methods: In this research, 24 male NMRI mice were divided into three groups including group A: control animal receiving vehicle; group B: the group receiving basal diet and TX 0.4 mg/kg/day; and group C: the group receiving basal diet and TX 0.6 mg/kg/day for 35 days. Thereafter, epididymal spermatozoa were analyzed for standard parameters and nuclear chromatin quality using Aniline Blue (AB) and Toluidine Blue (TB) staining.
Results: The results indicated that although the TX did not affect the sperm count, motility, and viability parameters, it could elevate the percentage of sperm cells with abnormal morphology and abnormal chromatin at both doses. In addition, in comparison with the control mice, a significant elevation was observed in spermatozoa with residual histones (assessed by AB staining) at high doses of TX.
Conclusion: Our experimental data in mice suggested that the use of TX for treating male infertility might increase the rates of spermatozoa with abnormal chromatin in a dose-dependent manner.
Full-Text [PDF 806 kb]   (842 Downloads) |   |   Full-Text (HTML)  (494 Views)  
Type of Study: Original Article |

1. [1] Stephen EH, Chandra A. Updated projections of infertility in the United States: 1995-2025. Fertil Steril 1998; 70: 30-34. [DOI:10.1016/S0015-0282(98)00103-4]
2. [2] Oehninger S, Kruger T. Male infertility: diagnosis and treatment. Taylor & Francis US; 2007. [DOI:10.3109/9780203090626] [PMCID]
3. [3] Moein MR, Tabibnejad N, Ghasemzadeh J. Beneficial effect of tamoxifen on sperm recovery in infertile men with nonobstructive azoospermia. Andrologia 2012; 44(Suppl.): 194-198. [DOI:10.1111/j.1439-0272.2011.01163.x] [PMID]
4. [4] Traub AL, Thompson W. The effect of tamoxifen on spermatogenesis in subfertile men. Andrologia 1981; 13: 486-490. [DOI:10.1111/j.1439-0272.1981.tb00087.x] [PMID]
5. [5] Huang CC, Lin DP, Tsao HM, Cheng TC, Liu CH, Lee MS. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril 2005; 84: 130-140. [DOI:10.1016/j.fertnstert.2004.08.042] [PMID]
6. [6] Balhorn R. A model for the structure of chromatin in mammalian sperm. J Cell Biol 1982; 93: 298-305. [DOI:10.1083/jcb.93.2.298] [PMID] [PMCID]
7. [7] Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 2008; 40: 245-251. [DOI:10.1111/j.1439-0272.2008.00852.x] [PMID]
8. [8] Amaral S, Oliveira PJ, Ramalho-Santos J. Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive oxygen species. Curr Diabetes Rev 2008; 4: 46-54. [DOI:10.2174/157339908783502398] [PMID]
9. [9] Talebi AR, Khalili MA, Nahangi H, Abbasi A, Anvari M. Evaluation of epididymal necrospermia following experimental chronic spinal cord injury in rat 2007. Iran J Reprod Med; 5: 171-176.
10. [10] Talebi AR, Vahidi S, Aflatoonian A, Ghasemi N, Ghasemzadeh J, Dehghani Firoozabadi R, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia 2012; 44 (Suppl.): [DOI:10.1111/j.1439-0272.2011.01206.x] [PMID]
12. [11] Oryan S, Parivar K, Asle Rousta M. Effect of tamoxifen on histological structure of testis in adult male wistar rats. Med Sci J Islamic Azad Univ Tehran Med Branch 2008; 18: 81- 84. (in Persian)
13. [12] Aleem M, Padwal V, Choudhari J, Balasinor N, Parte P, Gill−Sharma M. Effects of tamoxifen citrate on gene expression during nuclear chromatin condensation in male rats. Asian J Androl 2005; 7: 311-321. [DOI:10.1111/j.1745-7262.2005.00027.x] [PMID]
14. [13] Motrich RD, Ponce AA, Rivero VE. Effect of tamoxifen treatment on the semen quality and fertility of the male rat. Fertil Steril 2007; 88: 452-461. [DOI:10.1016/j.fertnstert.2006.11.196] [PMID]
15. [14] Corrada Y, Arias D, Rodriguez R, Spaini E, Fava F, Gobello C. Effect of tamoxifen citrate on reproductive parameters of male dogs. Theriogenology 2004; 61: 1327-1341. [DOI:10.1016/j.theriogenology.2003.07.020] [PMID]
16. [15] Nada EA, El Taieb MA, Ibrahim HM, Al Saied AE. Efficacy of tamoxifen and l−carnitine on sperm ultrastructure and seminal oxidative stress in patients with idiopathic oligoasthenoteratozoospermia. Andrologia 2015; 47: 801- 810. [DOI:10.1111/and.12333] [PMID]
17. [16] Chua ME, Escusa KG, Luna S, Tapia LC, Dofitas B, Morales M. Revisiting oestrogen antagonists (clomiphene or tamoxifen) as medical empiric therapy for idiopathic male infertility: a meta−analysis. Andrology 2013; 1: 749- 757. [DOI:10.1111/j.2047-2927.2013.00107.x] [PMID]
18. [17] Talebi AR, Fesahat F, Mangoli E, Ghasemzadeh J, Nayeri M, Sadeghian-Nodoshan F. Relationship between sperm protamine deficiency and apoptosis in couples with unexplained repeated spontaneous abortions. Int J Reprod BioMed (Yazd) 2016; 14: 199-204. [DOI:10.29252/ijrm.14.3.199]
19. [18] Pathak S, Kedia-Mokashi N, Saxena M, D'Souza R, Maitra A, Parte P, et al. Effect of tamoxifen treatment on global and insulin-like growth factor 2-H19 locus-specific DNA methylation in rat spermatozoa and its association with embryo loss. Fertil Steril 2009; 91: 2253-2263. [DOI:10.1016/j.fertnstert.2008.07.1709] [PMID]
20. [19] Balasinor N, Gill-Sharma MK, Parte P, D'Souza S, Kedia N, Juneja HS. Effect of paternal administration of an antiestrogen, tamoxifen on embryo development in rats. Mol Cell Endocrinol t2002; 190: 159-166. [DOI:10.1016/S0303-7207(01)00723-7]

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb