International Journal of
Reproductive Biomedicine
Tue, Dec 3, 2024
[Archive]
Volume 19, Issue 2 (February 2021)
IJRM 2021, 19(2): 137-146 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yusuf I, Abiodun Emokpae M. Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study. IJRM 2021; 19 (2) :137-146
URL: http://ijrm.ir/article-1-1617-en.html
URL: http://ijrm.ir/article-1-1617-en.html
1- Department of Medical Laboratory Science, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria. , mathias.emokpae@uniben
2- Department of Medical Laboratory Science, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria.
2- Department of Medical Laboratory Science, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria.
Abstract: (1820 Views)
Background: Studies have shown oxidative DNA damage is associated with male infertility.
Objective: This study determines the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and some markers of oxidative stress in seminal fluid of males investigated for infertility and men of proven fertility in Benin City, Nigeria.
Materials and Methods: Semen samples produced by self or assisted masturbation were analyzed by microscopic technique according to the World Health Organization guidelines. Thereafter, samples were centrifuged and seminal fluid plasma separated and stored at -20ºC prior to assay for 8-OHdG and oxidative stress biomarkers. Based on the sperm concentration/count, the overall samples were grouped into the following categories: normospermia (n = 20), oligozoospermia (n = 30), and azoospermia (n = 20). The control group comprised of 30 age-matched males of proven fertility. The seminal fluid 8-OHdG, total antioxidant status, superoxide dismutase and malondialdehyde (MDA) were assayed through ELISA and spectrophotometric methods, respectively.
Results: Seminal plasma level of 8-OHdG and MDA were significantly higher (p = 0.01) in infertile subjects than controls. The mean levels of 8-OHdG and MDA in infertile subjects were higher in azoospermia than oligospermia than normospermia and so, was least in the normospermia. Conversely, the mean levels of total antioxidant status and superoxide dismutase were significantly lower (p = 0.01) in infertile than fertile the control male subjects with levels higher in normospermia than oligospermia and least in azoospermia. Moreover, the seminal 8-OHdG correlated negatively with sperm count (r = -0.359, p = 0.01), percent motility (r = -0.388, p = 0.04), and percent morphology (r = -0.327, p = 0.02).
Conclusion: The assessment of sperm DNA damage in addition to routine seminal fluid analysis may play an important role in specific diagnosis and management of male infertility.
Objective: This study determines the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and some markers of oxidative stress in seminal fluid of males investigated for infertility and men of proven fertility in Benin City, Nigeria.
Materials and Methods: Semen samples produced by self or assisted masturbation were analyzed by microscopic technique according to the World Health Organization guidelines. Thereafter, samples were centrifuged and seminal fluid plasma separated and stored at -20ºC prior to assay for 8-OHdG and oxidative stress biomarkers. Based on the sperm concentration/count, the overall samples were grouped into the following categories: normospermia (n = 20), oligozoospermia (n = 30), and azoospermia (n = 20). The control group comprised of 30 age-matched males of proven fertility. The seminal fluid 8-OHdG, total antioxidant status, superoxide dismutase and malondialdehyde (MDA) were assayed through ELISA and spectrophotometric methods, respectively.
Results: Seminal plasma level of 8-OHdG and MDA were significantly higher (p = 0.01) in infertile subjects than controls. The mean levels of 8-OHdG and MDA in infertile subjects were higher in azoospermia than oligospermia than normospermia and so, was least in the normospermia. Conversely, the mean levels of total antioxidant status and superoxide dismutase were significantly lower (p = 0.01) in infertile than fertile the control male subjects with levels higher in normospermia than oligospermia and least in azoospermia. Moreover, the seminal 8-OHdG correlated negatively with sperm count (r = -0.359, p = 0.01), percent motility (r = -0.388, p = 0.04), and percent morphology (r = -0.327, p = 0.02).
Conclusion: The assessment of sperm DNA damage in addition to routine seminal fluid analysis may play an important role in specific diagnosis and management of male infertility.
Type of Study: Original Article |
Subject:
Reproductive Andrology
References
1. 1. Gharreb DA, Sarhan EME. Role of oxidative stress in male fertility and Idiopathic infertility: Causes and treatment. J Diagn Tech Biomed Anal 2014; 3: 1000110.
2. 2. Guzick DS, Overstreet JW, Factor-Litvak P, Brazil CK, Nakajima ST, Coutifaris C. Sperm morphology, motility, and concentration in fertile and infertile men. N Engl J Med 2001; 345: 1388-1393.
3. 3. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Ferti Steril 2001; 75: 674-677.
4. 4. Schulte RT, Ohl DA, Sigman M, Smith GD. Sperm DNA damage in male infertility: Etiologies, assays and outcomes. J Assist Reprod Genet 2010; 27: 3-12.
5. 5. Agarwal A, Allamaneni ShSR. Sperm DNA damage assessment: a test whose time has come. Fertil Steril 2005; 84: 850-853.
6. 6. Uadia PO, Emokpae AM. Male infertility in Nigeria: A neglected reproductive health issue requiring attention. J Basic Clin Reprod Sci 2015; 4: 45-53.
7. 7. Emokpae MA, Uadia PO. Potential risk of senescence on male fertility and sperm DNA damage on progeny. Trop J Nat Prod Res 2017; 1: 58-62.
8. 8. Jungwirth A, Diemer T, Dohle GR, Giwercman A, Kopa Z, Krausz C, et al. Guidelines on male infertility. Eur Urol 2015; 62: 324-332.
9. 9. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th Ed. Geneva: World Health Organization; 2010.
10. 10. Ni K, Steger K, Yang H, Wang H, Hu K, Zhang T, et al. A comprehensive investigation of sperm DNA damage and oxidative stress injury in infertile patients with subclinical, normozoospermic, and astheno/oligozoospermic clinical varicocoele. Andrology 2016; 4: 816-824.
11. 11. Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. Etiologies of sperm oxidative stress. Int J Reprod Biomed 2016; 14: 231-240.
12. 12. Bisht Sh, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nat Rev Urol 2017; 14: 470-485.
13. 13. Wagner H, Cheng JW, Ko EY. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol 2017; 16: 35-43.
14. 14. Alahmar AT. Role of oxidative stress in male infertility: An update review. J Hum Reprod Sci 2019; 12: 4-18.
15. 15. Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HWG, Behre HM, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010; 16: 231-245.
16. 16. Chukudebelu WO. The male factor in infertility - Nigerian experience. Int J Fertil 1978; 23: 238-239.
17. 17. Uadia PO, Emokpae MA. Implications of oxidative stress on male infertility. Trans Niger Soc Biochem Mol Biol 2015; 1: 19-29.
18. 18. Emokpae MA, Chima HN, Ahmed M. Seminal plasma caspase 3, cytochrome c and total antioxidant capacity in oligospermic males and association with sperm indices. J Exp Integr Med 2016; 6: 1-4.
19. 19. Emokpae MA, Uadia PO, Omole-Ohonsi A. Pattern of hormonal abnormalities and association with sperm parameters among oligospermic male partners of infertile couples. Niger Endocrin Pract 2014; 8: 13-19.
20. 20. Aydos OS, Yukselten Y, Kaplan F, Sunguroglu A, Aydos K. Analysis of the correlation between sperm DNA integrity and conventional semen parameters in infertile men. Turk J Urol 2015; 41: 191-197.
21. 21. Evgeni E, Lymberopoulos G, Gazouli M, Asimakopoulos B. Conventional semen parameters and DNA fragmentation in relation to fertility status in a Greek population. Eur J Obstet Gynecol Reprod Biol 2015; 188: 17-23.
22. 22. Majzoub A, Arafa M, Mahdi M, Agarwal A, Al Said S, Al-Emadi I, et al. Oxidation-reduction potential and sperm DNA fragmentation, and their associations with sperm morphological anomalies amongst fertile and infertile men. Arab J Urol 2018; 16: 87-95.
23. 23. Host E, Lindenberg S, Smidt-Jensen S. The role of DNA strand breaks in human spermatozoa used for IVF and ICSI. Acta Obstet Gynecol Scand 2000; 79: 559-563.
24. 24. Emokpae MA, Chima HN, Ahmed M. Seminal Caspase 3, cytochrome c and total antioxidant capacity in Nigerian male subjects undergoing infertility evaluation. Afr J Biomed Res 2019; 22: 59-63.
25. 25. Ombelet W, Wouters E, Boels L, Cox A, Janssen M, Spiessens C, et al. Sperm morphology assessment: diagnostic potential and comparative analysis of strict or WHO criteria in a fertile and a subfertile population. Int J Androl 1997; 20: 367-372.
26. 26. Agarwal A, Said TM. Oxidative stress, DNA damage and apoptosis in male infertility: A clinical approach. BJU Int 2005; 95: 503-507.
27. 27. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod 2000; 15: 1338-1344.
28. 28. Aitken RJ, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction 2001; 122: 497-506.
29. 29. Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, et al. Characterization of subsets of human spermatozoa at different stages of maturation: Implications in the diagnosis and treatment of male infertility. Hum Reprod 2001; 16: 1912-1921.
30. 30. Fischer MA, Willis J, Zini A. Human sperm DNA integrity: Correlation with sperm cytoplasmic droplets. Urology 2003; 61: 207-211.
31. 31. Aitken RJ, Curry BJ. Redox regulation of human sperm function: From the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line. Antioxid Redox Signal 2011; 14: 367-381.
32. 32. Burruel V, Klooster KL, Chitwood J, Ross PJ, Meyers SA. Oxidative damage to rhesus macaque spermatozoa results in mitotic arrest and transcript abundance changes in early embryos. Biol Reprod 2013; 89: 72.
33. 33. Moazamian R, Polhemus A, Connaughton H, Fraser B, Whiting S, Gharagozloo P, et al. Oxidative stress and human spermatozoa: diagnostic and functional significance of aldehydes generated as a result of lipid peroxidation. Mole Hum Reprod 2015; 21: 502-515.
34. 34. Mruk DD, Cheng CY. In vitro regulation of extracellular superoxide dismutase in sertoli cells. Life Sci 2000; 67: 133-145.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
