Volume 19, Issue 1 (January 2021)                   IJRM 2021, 19(1): 23-34 | Back to browse issues page

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Najafipour R, Momeni A, Yousefipour F, Mousavi S, Moghbelinejad S. Underexpression of hsa-miR-449 family and their promoter hypermethylation in infertile men: A case-control study. IJRM 2021; 19 (1) :23-34
URL: http://ijrm.ir/article-1-1628-en.html
Underexpression of hsa-miR-449 family and their promoter hypermethylation in infertile men: A case-control study
Reza Najafipour1 , Abdolmabood Momeni2 , Farideh Yousefipour3 , Shaghayegh Mousavi4 , Sahar Moghbelinejad * 5
1- Research Institute for Prevention of Non-Communicable Diseases, Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran.
2- Biology Department, School of Basic Science, Arak University, Arak, Iran.
3- National Institute of Engineering and Biotechnology, Tehran, Iran.
4- Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
5- Research Institute for Prevention of Non-Communicable Diseases, Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran. , smoghbelinejad@qums.ac.ir
Abstract:   (1811 Views)
Background: Post-transcriptional microRNAs (miRNAs) have a impotrant pattern in the spermatogenesis process.
Objective: Study of the expression and methylation of hsa-miR-449 family in sperm samples of infertile men.
Materials and Methods: In this case-control study, we recruited 74 infertile men (with asthenozoospermia, teratozoospermia, asthenoteratozoospermia, and oligoasthenoteratozoospermia) and 30 control samles. Methylation-specific PCR (MSP) method was used for methylation evaluation of hsa-miR-449 a, b, c promoter. By Real time PCR (qRT-PCR) method,we showed downregulation of hsa-miR-449 a, b, c in the sperm samples of infertile men and compared it to their fertile counterparts.
Results: There was significant underexperssion, in hsa-miR-449-b in oligoasthenoteratospermic samples (p = 0.0001, F = 2.9). About the methylation pattern, infertile men showed high frequency of methylation in the promoter of hsa-miR-449 a, b, c in comparison to controls (60.8% vs 23.3%), the highest amount of methylation was observed in oligoasthenoteratospermia samples (81.2%).
Conclusion: In this study, low expression and high methylation of hsa-miR-449-b were observed in infertile men in compared to control samples, which can be one of the causes of defective spermatogenesis.
Keywords: Spermatogenesis, miR-449, Expression, Epigenetic.
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Type of Study: Original Article | Subject: Reproductive Genetics
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