Volume 18, Issue 12 (December 2020)                   IJRM 2020, 18(12): 1073-1080 | Back to browse issues page

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Tajabadi E, Javadi A, Ahmadi Azar N, Najafi M, Shirazi A, Shabeeb D et al . Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study. IJRM 2020; 18 (12) :1073-1080
URL: http://ijrm.ir/article-1-1629-en.html
Radioprotective effect of a combination of melatonin and metformin on mice spermatogenesis: A histological study
Elham Tajabadi1 , Abdolreza Javadi2 , Nasim Ahmadi Azar1 , Masoud Najafi * 3, Alireza Shirazi4 , Dheyauldeen Shabeeb5 , Ahmed Eleojo Musa6
1- Department of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Medical Technology Research Center, Institute of Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran. Radiology and Nuclear Medicine Department, School of Paramedical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran. , najafi_ma@yahoo.com
4- Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Radiation Oncology Department, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
5- Department of Physiology, College of Medicine, University of Misan, Misan, Iraq.
6- Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences (International Campus), Tehran, Iran.
Abstract:   (1966 Views)
Background: The spermatogenesis system includes highly radiosensitive cells. Hence, this system is a potential target for toxic effects of ionizing radiation during radiotherapy of abdomen and pelvis cancers, as well as after accidental radiation events. Accordingly, metformin and melatonin are two important radioprotectors that have shown an ability to prevent cell death through neutralization of free radicals and stimulating DNA damage responses.
Objective: To evaluate the radioprotective effects of melatonin and metformin on mice spermatogenesis when administered alone or as a combination.
Materials and Methods: In this histological Study, 40 (6-8 wk, 30 gr) NMRI mice were divided into 8 groups (n = 5/each) as control, metformin, melatonin, melatonin + metformin, radiation, radiation + melatonin, radiation + metformin, and radiation + melatonin + metformin. 37 days after the irradiation, the testicular tissues were collected for histological evaluation.
Results: Single administration of melatonin could ameliorate effectively radiation toxicity in mice testis. Metformin showed radioprotective effects on some parameters such as the numbers of spermatogonia and mature sperms. Interestingly, the melatonin and metformin combination reversed the reduced number of sperms rather than single drug administration.
Conclusion: The combination of melatonin with metformin can protect mice spermatogenesis against ionizing radiation more effectively compared to the single forms of these drugs.
Keywords: Radiation, Testis, Leydig cells, Melatonin, Metformin, Spermatogenesis.
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Type of Study: Original Article | Subject: Reproductive Pattology
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