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Soleimani Mehranjani M, Noorafshan A, Hamta A, Momeni H R, Abnosi M H, Mahmoodi M, et al . Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: A stereological study. IJRM 2010; 8 (1) :1-9
URL: http://ijrm.ir/article-1-172-en.html
Effects of vitamin E on ovarian tissue of rats following treatment with p-nonylphenol: A stereological study
Malek Soleimani Mehranjani * 1, Ali Noorafshan2 , Ahmad Hamta3 , Hamid Reza Momeni3 , Mohammad Hussein Abnosi3 , Monireh Mahmoodi3 , Morteza Anvari4 , Maryam Hazaveh3
1- Department of Biology, Faculty of Sciences, Arak University of Medical Sciences, Arak, Iran , m-soleimani@araku.ac.ir
2- Department of Anatomy, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3- Department of Biology, Faculty of Sciences, Arak University of Medical Sciences, Arak, Iran
4- Department of Anatomy, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Abstract:   (2437 Views)
Background: Para-Nonylphenol (p-NP) is one of the environmental pollutants which cause reproductive system disorders.
Objective: The effects of vitamin E on ovary structure during its development in rats treated with p-NP.
Materials and Methods: 32 Wistar female rats after mating were divided into 4 groups; control vitamin E (100mg/kg/day) p-NP (250mg/kg/day) and p-NP + vitamin E. The rats were treated from the day 7 of pregnancy till 21st day of postnatal through sucking period. After weaning the female pups were treated by gavages for 120 days. The total volume of ovary number of follicles volume of oocyte follicular cells and their nuclei and the thickness of zona pellucida were estimated stereologically. The results were analyzed using one way ANOVA and p<0.05 was considered significant.
Results: The ovary weight, mean total volume of ovary and cortex, number of antral and graafian follicles and body weight were decreased significantly (p<0.05) in the p-NP treated rats compared to control and other groups, while the number of atretic follicles was increased significantly (p<0.05). A significant reduction (p<0.05) in volume of oocyte, follicular cells and their nuclei in antral and graafian follicles was found in p-NP group. In addition, treatment with only vitamin E showed an improving effect on folliculogenesis due to a highly significant increase (p<0.01) in the number of primordial follicles.
Conclusion: Vitamin E could compensate the adverse effects of p-NP on the ovary structure during its development.
Keywords: Ovary p-Nonylphenol Rat Stereology Vitamin E.
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Type of Study: Original Article |
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