International Journal of
Reproductive Biomedicine
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Volume 19, Issue 4 (April 2021)
Int J Reprod BioMed. 2021, 19(4): 371-380 |
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Azari M, Kafi M, Asaadi A, Pakniat Z, Abouhamzeh B. Bovine oocyte developmental competence and gene expression following co-culturing with ampullary cells: An experimental study. Int J Reprod BioMed. 2021; 19 (4) :371-380
URL: http://ijrm.ir/article-1-1788-en.html
URL: http://ijrm.ir/article-1-1788-en.html
1- Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Department of Anatomical Sciences, Faculty of Medicine, AJA University of Medical Sciences, Sarhang Etemadzadzadeh St., West Fatemi St., Tehran, Iran. Postal code: 1411718541
2- Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Department of Anatomical Sciences, Faculty of Medicine, AJA University of Medical Sciences, Sarhang Etemadzadzadeh St., West Fatemi St., Tehran, Iran. Postal code: 1411718541
3- Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. ,b.abouhamzeh.ba@gmail.com
2- Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran., Department of Anatomical Sciences, Faculty of Medicine, AJA University of Medical Sciences, Sarhang Etemadzadzadeh St., West Fatemi St., Tehran, Iran. Postal code: 1411718541
3- Department of Anatomical Sciences, School of Medicine, AJA University of Medical Sciences, Tehran, Iran. ,
Abstract: (2984 Views)
Background: There is no sufficient information on the impact of bovine ampullary oviductal epithelial cells (BAOECs) on in vitro oocyte maturation competence and gene expression.
Objective: This study aimed to examine the oocyte developmental competence following co-culturing with a monolayer of fresh and frozen-thawed ampullary cells.
Materials and Methods: Bovine cumulus-oocyte complexes (COCs) were distributed into three groups: control group; where in COCs were cultured in cell-free media for 24 hr and FML and FTML groups in which the COCs were cultured in maturation media for 18 hr and then transferred into a media containing fresh and frozen-thawed BAOECs monolayer, respectively (BAOECs were extracted from the oviducts of slaughtered cattle and were then cultured freshly or frozen-thawed) for a further 6 hr. After 24 hr, the expanded COCs were evaluated for nuclear maturation, fertilization rate, and gene expression (GDF9, StAR, CASP3, and FSHr).
Results: Nuclear maturation rate in the FTML group was significantly higher than the control group (p = 0.02). The fertilization rate of FTML group was significantly higher than the control and FML groups (p = 0.05 and p = 0.03, respectively). In terms of gene expression, GDF9 were upregulated in the presence of the BAOECs during the last 6 hr of the in vitro maturation (p < 0.001). Furthermore, the expression of the StAR gene in the FTML group was higher than the other groups (p = 0.02).
Conclusion: Ampullary cells co-culturing (especially frozen-thawed cells) for in vitro maturation of bovine oocytes yields encourages the results and demonstrates the beneficial effect of co-culture on gene expression and developmental competence.
Objective: This study aimed to examine the oocyte developmental competence following co-culturing with a monolayer of fresh and frozen-thawed ampullary cells.
Materials and Methods: Bovine cumulus-oocyte complexes (COCs) were distributed into three groups: control group; where in COCs were cultured in cell-free media for 24 hr and FML and FTML groups in which the COCs were cultured in maturation media for 18 hr and then transferred into a media containing fresh and frozen-thawed BAOECs monolayer, respectively (BAOECs were extracted from the oviducts of slaughtered cattle and were then cultured freshly or frozen-thawed) for a further 6 hr. After 24 hr, the expanded COCs were evaluated for nuclear maturation, fertilization rate, and gene expression (GDF9, StAR, CASP3, and FSHr).
Results: Nuclear maturation rate in the FTML group was significantly higher than the control group (p = 0.02). The fertilization rate of FTML group was significantly higher than the control and FML groups (p = 0.05 and p = 0.03, respectively). In terms of gene expression, GDF9 were upregulated in the presence of the BAOECs during the last 6 hr of the in vitro maturation (p < 0.001). Furthermore, the expression of the StAR gene in the FTML group was higher than the other groups (p = 0.02).
Conclusion: Ampullary cells co-culturing (especially frozen-thawed cells) for in vitro maturation of bovine oocytes yields encourages the results and demonstrates the beneficial effect of co-culture on gene expression and developmental competence.
Type of Study: Original Article |
Subject:
Assisted Reproductive Technologies
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