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Volume 19, Issue 6 (June 2021)
IJRM 2021, 19(6): 515-524 |
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Khazaei F, Ghanbari E, Khazaei M. Improved hormonal and oxidative changes by Royal Jelly in the rat model of PCOS: An experimental study. IJRM 2021; 19 (6) :515-524
URL: http://ijrm.ir/article-1-1793-en.html
URL: http://ijrm.ir/article-1-1793-en.html
1- Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. , mkhazaei1345@yahoo.com
2- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3- Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. , mkhazaei1345@yahoo.com
Abstract: (1540 Views)
Background: Polycystic ovarian syndrome (PCOS) is an endocrine and complex metabolic disorder, associated with anovulation, changes in sex hormone, biochemical factors, and ovarian tissue. Royal jelly (RJ) has antioxidant and anti-inflammatory properties.
Objective: To examine the therapeutic effect of RJ on PCOS-related hormonal and biochemical changes in a rat model of PCOS.
Materials and Methods: In this experimental study, 42 female Wistar rats (weighing 180-200 gr, aged 10-12 week) were divided into six groups (n = 7/each): control; PCOS; RJ 100 mg/kg; RJ 200 mg/kg; PCOS + RJ 100 mg/kg; and PCOS + RJ 200 mg/kg. After 21 days, the animals were weighed and dissected. The serums were used for nitric oxide (NO) and ferric-reducing antioxidant power (FRAP) assay and estradiol and progesterone measurements. The ovaries were assessed for histological changes.
Results: PCOS increased estradiol and NO levels, and decreased progesterone and FRAP levels. In PCOS+ RJ groups, the progesterone (p =0.01) and FRAP levels (p ≤ 0.001) increased and the estradiol and NO (p ≤ 0.001) levels decreased significantly. Moreover, the number of mature follicles (p = 0.01) and corpus luteum increased (p ≤ 0.001), and ovarian and uterus weight deceased significantly (p ≤ 0.001).
Conclusion: RJ improved estradiol, progesterone, FRAP, and NO levels, and ovarian structure in the rat model of PCOS.
Objective: To examine the therapeutic effect of RJ on PCOS-related hormonal and biochemical changes in a rat model of PCOS.
Materials and Methods: In this experimental study, 42 female Wistar rats (weighing 180-200 gr, aged 10-12 week) were divided into six groups (n = 7/each): control; PCOS; RJ 100 mg/kg; RJ 200 mg/kg; PCOS + RJ 100 mg/kg; and PCOS + RJ 200 mg/kg. After 21 days, the animals were weighed and dissected. The serums were used for nitric oxide (NO) and ferric-reducing antioxidant power (FRAP) assay and estradiol and progesterone measurements. The ovaries were assessed for histological changes.
Results: PCOS increased estradiol and NO levels, and decreased progesterone and FRAP levels. In PCOS+ RJ groups, the progesterone (p =0.01) and FRAP levels (p ≤ 0.001) increased and the estradiol and NO (p ≤ 0.001) levels decreased significantly. Moreover, the number of mature follicles (p = 0.01) and corpus luteum increased (p ≤ 0.001), and ovarian and uterus weight deceased significantly (p ≤ 0.001).
Conclusion: RJ improved estradiol, progesterone, FRAP, and NO levels, and ovarian structure in the rat model of PCOS.
Type of Study: Original Article |
Subject:
Fertility & Infertility
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