Volume 19, Issue 10 (October 2021)                   IJRM 2021, 19(10): 921-928 | Back to browse issues page

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Banafshi O, Nasseri S, Farhadi L, Alasvand M, Khadem-Erfan M B, Hosseini J, et al . The effects of supplemented sericin on in vitro maturation and preimplantation development of mouse embryos: An experimental study. IJRM 2021; 19 (10) :921-928
URL: http://ijrm.ir/article-1-1795-en.html
The effects of supplemented sericin on in vitro maturation and preimplantation development of mouse embryos: An experimental study
Omid Banafshi1 , Sherko Nasseri1 , Leila Farhadi1 , Masoud Alasvand2 , Mohammad Bagher Khadem-Erfan1 , Javad Hosseini1 , Saber Miraki1 , Fardin Fathi * 3
1- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
3- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. , farfath@gmail.com
Abstract:   (2004 Views)
Background: Mouse embryo culture condition is an essential part of transgenic, reproductive and developmental biology laboratories. Mouse embryonic culture media may have a high risk of serum contamination with pathogens.
Objective: To investigated the effect of sericin as an embryo culture medium supplement on in vitro maturation (IVM), in vitro fertilization (IVF), and development of the preimplantation embryo in mice.
Materials and Methods: The effects of sericin at three concentrations (subgroups) of 0.1%, 0.5%, and 1% as a medium supplement on IVM, IVF, and in vitro development of mouse embryos were separately investigated and compared with a sericin-free (control) group. The cumulative effect of the three concentrations was evaluated for IVM+ in vitro development and IVF+ in vitro development as follow-up groups.
Results: In the IVM group, compared to the control group, the number of oocysts reaching the MII stage was significantly higher when 1% sericin was used (161/208 = 77.4%). No significant results were observed in the IVF and in vitro development groups with different concentrations of sericin compared to the control group. Among the follow-up groups, in the IVM + in vitro development group, the number of oocytes was higher after passing the IVM and IVF and reaching the blastocysts stage when 1% sericin was used, compared with other sericin subgroups. A significant difference was also noted when compared with the control group (p = 0.048). The IVF+ in vitro development study group, on the other hand, did not show any significant relationship.
Conclusion: It can be concluded that 1% sericin can be used as a supplement in mouse embryo cultures to improve the IVM rate. Also, based on the findings, sericin appears to be an effective supplement which can have a positive effect on the development of embryos derived from IVM.
Keywords: Sericin, In vitro maturation, In vitro fertilization, Preimplantation embryo, Culture medium, Mice.
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Type of Study: Original Article | Subject: Assisted Reproductive Technologies
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