چهارشنبه 12 اردیبهشت 1403
[Archive]
دوره 8، شماره 3 - ( 4-1389 )
جلد 8 شماره 3 صفحات 110-101 |
برگشت به فهرست نسخه ها
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Razi M, Akhtari K, Najafpour A R, Abdi K, Shahrooz R, Shahmohamadloo S, et al . Effect of bilateral uterine artery ligation on follicular atresia in ovaries of mature female rabbits; histomorphometric and histochemical study. IJRM 2010; 8 (3) :101-110
URL: http://ijrm.ir/article-1-181-fa.html
URL: http://ijrm.ir/article-1-181-fa.html
Effect of bilateral uterine artery ligation on follicular atresia in ovaries of mature female rabbits; histomorphometric and histochemical study. International Journal of Reproductive BioMedicine. 1389; 8 (3) :101-110
چکیده: (2150 مشاهده)
Background: Nowadays it is proofed that the uterine artery plays essential role in follicular growth and/or post parturition hemorrhagic.
Objective: This study was conducted to evaluate the effect of Bilateral Uterine Artery Ligation (BUAL) on follicular fate and the probable histochemical changes of the carbohydrate and lipids in the ovaries of rabbits.
Materials and Methods: 24 mature female rabbits randomized into two test and control-sham groups. Test group subdivided to three groups based on time. Animals in the test group under went to BUAL. The ovaries were processed to histochemical and histomorphometric analyses to evaluate the ratio of lipid carbohydrate and lipase enzyme in follicular cells.
Results: The ovaries from test groups exhibited many atretic follicles in various sizes. BUAL significantly (p≤0.05) increased the rate of atresia in the test groups in comparison to the control-sham cases. This situation was progressed by the time. In the test groups lipid reactions were observed more remarkable in the small atretic follicles in comparison to the large atretic follicles. BUAL elevated the reaction sites for lipase enzyme in the early stages of the atresia in the test group.
Conclusion: Referring to our results BUAL caused significant (p≤0.05) hypo-ovulation by increasing the atresia. Also increasing lipid foci in the first stages of the apoptotic process caused cytoplasmic lipase enzyme evaluation while the lipase enzyme level was decreased by the advancement of the atresia and decreasing of the biological activities in follicular cells.
Objective: This study was conducted to evaluate the effect of Bilateral Uterine Artery Ligation (BUAL) on follicular fate and the probable histochemical changes of the carbohydrate and lipids in the ovaries of rabbits.
Materials and Methods: 24 mature female rabbits randomized into two test and control-sham groups. Test group subdivided to three groups based on time. Animals in the test group under went to BUAL. The ovaries were processed to histochemical and histomorphometric analyses to evaluate the ratio of lipid carbohydrate and lipase enzyme in follicular cells.
Results: The ovaries from test groups exhibited many atretic follicles in various sizes. BUAL significantly (p≤0.05) increased the rate of atresia in the test groups in comparison to the control-sham cases. This situation was progressed by the time. In the test groups lipid reactions were observed more remarkable in the small atretic follicles in comparison to the large atretic follicles. BUAL elevated the reaction sites for lipase enzyme in the early stages of the atresia in the test group.
Conclusion: Referring to our results BUAL caused significant (p≤0.05) hypo-ovulation by increasing the atresia. Also increasing lipid foci in the first stages of the apoptotic process caused cytoplasmic lipase enzyme evaluation while the lipase enzyme level was decreased by the advancement of the atresia and decreasing of the biological activities in follicular cells.
نوع مطالعه: Original Article |
فهرست منابع
1. 1 Kuscu E, Duran HE, Zeyneloglu HB, Demirhan B, Bagis T, Saygili E. The effect of surgical sterilization on ovarian function: a Rat model. Eur J Obset Gynecol Reprod Biol 2002; 100: 204-207. [DOI:10.1016/S0301-2115(01)00481-X]
2. 2 Kelekci S, Yorgancioglu Z, Yilmaz B, Yasar L, Savan K, Sonmez S, Kart C. Effect of tubal ligation on ovarian reveres and the ovarian stromal blood supply. Aus New Zeland Obstet Gyneco 2004; 44: 446-451.
3. 3 Tiars B, Noyan V, Ozdamer H, Guner H, Yildiz A, Yildirim M. The changes in ovarian hormone levels and ovarian artery blood flow rate after laparascopic tubal sterilization. Eur J Obset Gynecol Reprod Biol 2001; 99: 219-221. [DOI:10.1016/S0301-2115(01)00410-9]
4. 4 Ozdamer H, Ulger H, Sorkun I. effect of hysterectomy on ovarian morphology and serum FSH level in rat. Maturitas 2005, 52: 60-64. [DOI:10.1016/j.maturitas.2004.12.004]
5. 5 Kim HS, Thouse VR, Judson K, Vang R. Utero –Ovarian anastomosis: histopathologic correlation after uterine artery embolization with or without ovarian artery embolization. J Vet Interv Radiol 2007; 18: 9-31. [DOI:10.1016/j.jvir.2006.10.008]
6. 6 Petri Nahás EA, Pontes A, Nahas-Neto J, Borges VT, Dias R, Traiman P. Effect of total abdominal hysterectomy on ovarian blood supply in women of reproductive age. J Ultrasound Med 2005; 24: 169-174. [DOI:10.7863/jum.2005.24.2.169]
7. 7 Ahn EH, Bai SW, Song CH, Kim JY, Jeong KA, Kim SK, Lee JS, Kwon JY, Park KH. Effect of hysterectomy on conserved ovarian function. Yonsei Med J 2002; 43: 53-58. [DOI:10.3349/ymj.2002.43.1.53]
8. 8 Kelekci S, Yorgancioglu Z, Yilmaz B, Yasar L, Savan K, Sonmez S and Kart C. Effect of tubal ligation on ovarian reserve and the ovarian stromal blood supply. Australian & New Zeoland J Obes Gynecol 2004; 44: 449-451. [DOI:10.1111/j.1479-828X.2004.00269.x]
9. 9 Peppler R. effect of uterine artery ligation on ovulation in rat. The anatomic Record 1975: 184: 183-185.
10. 10 Ulf Z, Masato M, Matthew CP, Bengt N, Per-Olof J and Mats B. Evidence for the involvement of blood flow-related mechanisms in the ovulatory process of the rat. Hum Reprod 2000; 15: 264-272. [DOI:10.1093/humrep/15.2.264]
11. 11 Weherenberg WB, Dierschke DJ. The effect of ligation the ovarian and uterine arteries on ovarian function in rhesus monkeys. Biol Reprod 1979; 20: 596-600. [DOI:10.1095/biolreprod20.3.596]
12. 12 Luukkainen T. Contraception after thirty – five. Acta Obset Gynecol Scand 1992; 35: 164-174. [DOI:10.3109/00016349209009913]
13. 13 Hulka JF, Phillips JM, Peterson HB, Surrey MW. American association of gynecologic laparoscopic 1993 membership survey on. J Reprod Med 1990; 35: 584-586.
14. 14 Gretchen LH. Animal tissue techniques. 4th Ed. Philadelphia; W. H. Freeman Company; 1979.
15. 15 Ginter OJ. Comparative anatomy of utero ovarian vasculature. Scop 20 1976:1-17.
16. 16 Sumiala S, Pirhonen J, Tuminen J, Manenpaa J. Increased uterine and ovarian vascular resistant following fillshie clip sterilization: preliminary finding with Doppler ultrasonography. J Clin Ultrasound 1995; 23: 511-516. [DOI:10.1002/jcu.1870230902]
17. 17 Campbell S, Bourne T, Warwestone J. Transvaginal colour blood flow imaging of the periovulatory follicle. Fertil Steril 1993; 60: 433–438. [DOI:10.1016/S0015-0282(16)56156-1]
18. 18 Bjersing L, Cajander S. Ovulation and mechanisms of follicle rupture. Light microscopic changes in rabbit ovarian follicles prior to induced ovulation. Cell Tiss Res 1974; 149: 287–300. [DOI:10.1007/BF00226764]
19. 19 Blasco L, Wu CH, Ficklinger GL. Cardiac output and genital blood flow distribution during the preovulatory period in rabbits. Biol Reprod 1975; 13: 581–586 [DOI:10.1095/biolreprod13.5.581]
20. 20 Janson PO. Effects of the luteinizing hormone on blood flow in the follicular rabbit ovary, as measured by radioactive microspheres. Acta Endocrinol 1975; 79: 122–133. [DOI:10.1530/acta.0.0790122]
21. 21 Tanaka N, Espey LL, Okamura H. Increase in ovarian blood volume in the gonadotropin-primed rat. Biol Reprod 1989; 40: 762–768. [DOI:10.1095/biolreprod40.4.762]
22. 22 Natali R, Salvetti E, Gimeno J. Biochemical study of the extracellular matrix components in the follicular wall of induced polycystic ovaries. Braz J Morphol Sci 2003; 2:123-124.
23. 23 Lobo RA, Granger LR, Paul WL. Psycological stress and increases in urinary norepinephrine metabolites, platelets serotonin and adrenal androgens in women with polycystic ovarian syndrome. Am J Obset Gynecol 1983; 145: 496-503. [DOI:10.1016/0002-9378(83)90324-1]
24. 24 Lee GY, Croop JM and Anderson E. Multidrug resistance gene expression correlates with progesterone production in dehydroepiandrosterone-induced polycystic and Wquine chronic gonadotropin-stimulated ovaries of prepubertal rats. Biol Reprod 1998; 58: 330-337. [DOI:10.1095/biolreprod58.2.330]
25. 25 Aoltonen J, Laitinen MP, Voujolainen R, Jaatinen N, Horelli- kuitunen L, Seppa l, Human growth differentiation factor 9 (GDF9) and its novel homolg GDF-9B are expressed in oocyte during early folliculgenesis. J Clin Endocrinol Metab 1999; 84: 250-274.
26. 26 Najati V, Sadrkhanlou R and Hassanzadeh S. Histochemical study of stradiol Valerate- induced polycystic ovary in the rats. Iran J Vet Res 2006; 17: 68-76.
27. 27 Bath BK and Parshad RK. Mast cell dynamic in the hous rat (Rattus Rattus) ovary during estrus cycle, pregnancy and lactation. Eur J Morphol 2000; 38:17-23 [DOI:10.1076/ejom.38.1.0017]
28. 28 Ishida K. Histochemical study of lipase in rats ovaries with special reference to that in normal and atretic Ova. Tohoko J Agric Res 1953; 4: 133-139.
| بازنشر اطلاعات | |
 |
این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است. |
