Volume 19, Issue 8 (August 2021)                   IJRM 2021, 19(8): 699-706 | Back to browse issues page

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Afrakhteh H, Joharinia N, Momen A, Dowran R, Babaei A, Namdari P, et al . Relative frequency of hepatitis B virus, human papilloma virus, Epstein-Barr virus, and herpes simplex viruses in the semen of fertile and infertile men in Shiraz, Iran: A cross-sectional study. IJRM 2021; 19 (8) :699-706
URL: http://ijrm.ir/article-1-1820-en.html
Relative frequency of hepatitis B virus, human papilloma virus, Epstein-Barr virus, and herpes simplex viruses in the semen of fertile and infertile men in Shiraz, Iran: A cross-sectional study
Hossin Afrakhteh1 , Negar Joharinia1 , Akhtar Momen1 , Razieh Dowran1 , Abozar Babaei1 , Parisa Namdari1 , Mohammad Motamedifar2 , Behieh Namavar3 , Jamal Sarvari * 4
1- Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran. Shiraz HIV/AIDS Research Center, Institute of Health and Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3- Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4- Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, Iran. Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. , sarvarij@sums.ac.ir
Abstract:   (1688 Views)
Background: About 8-12% of couples on reproductive age suffers from infertility worldwide. Since 1993, the role of genital tract infections by microbes, including viruses that can infect the sperm, in human infertility has been proposed.
Objective: To investigate the frequency of hepatitis B virus (HBV), human papilloma virus (HPV), Epstein-Barr virus (EBV), and herpes simplex virus (HSV) infection in the semen of fertile and infertile men referred to the Mother and Child Hospital, Shiraz, Iran.
Materials and Methods: In this cross-sectional study, 350 men including 200 infertile and 150 fertile men were included. All semen samples were allowed to liquefy, followed by the assessment of sperm parameters. DNA was extracted using a DNA extraction kit (CinaGene, Tehran, Iran) according to the manufacturer’s instructions. Detection of HBV, HPV, EBV, and HSV1/2 was done by the PCR method.
Results: The mean age of the participants was 36 ± 7 yr. Molecular results showed that 16 samples (8%) of infertile men and 5 (3.3%) of fertile men were positive for HBV, which was not statistically significant (p = 0.069). Only one sample of the fertile participants was positive for HPV. None of the semen samples of the infertile or fertile groups was positive for the presence of EBV or HSV1/2.
Conclusion: The results of this study indicated that HBV, HPV, EBV, and HSV might not be involved in men’s infertility. Further studies are recommended for clarifying the role of these viruses in infertility.
Keywords: Male infertility, Hepatitis B virus, Human papilloma virus, Epstein-Barr virus, Herpes simplex viruses.
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Type of Study: Original Article | Subject: Fertility & Infertility
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