Volume 19, Issue 3 (March 2021)                   IJRM 2021, 19(3): 283-292 | Back to browse issues page

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Hosseini Sisi S Z, Azarbayjani M A, Vafaeenasab M, Peeri M, Dehghani M. The effect of regular resistance exercise, vitamin D, and calcium supplements on the gastrocnemius muscle in rats in the post-menopausal period: An experimental study. IJRM 2021; 19 (3) :283-292
URL: http://ijrm.ir/article-1-1879-en.html
The effect of regular resistance exercise, vitamin D, and calcium supplements on the gastrocnemius muscle in rats in the post-menopausal period: An experimental study
Seyedeh Zahra Hosseini Sisi1 , Mohammad Ali Azarbayjani * 2, Mohammadreza Vafaeenasab3 , Maghsoud Peeri1 , Mohammadreza Dehghani4
1- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. , m_azarbayjani@iauctb.ac.ir
3- Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4- Yazd Medical Genetic Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract:   (1911 Views)
Background: Menopause is the natural termination of menstruation which affects the quality and important aspects of women's life.
Objective: To evaluate the effect of regular resistance training (Ex) with vitamin D (Vit. D) and calcium (Ca) supplements in the postmenopausal period on muscle tissue in rats.
Materials and Methods: In this experimental study, 72 female Wistar rats (8-10-wk old) were randomly divided into control, placebo, Vit. D, Ca, Ex, Ca + Vit. D, Ex + Ca, Ex + Vit. D, and Ex + Ca + Vit. D groups. Control and placebo groups were fed with a standard diet and sesame oil, respectively. Two month after the ovariectomy, Ex, Ca (35 mg/kg), and Vit. D (10000 IU) were administred in all groups except the control. The number of muscle and inflammatory cells, fiber diameter, endomysium thickness, and degenerative collagen fiber area were assessed through hematoxylin-eosin staining.
Results: Muscle cell number was increased in the Ex + Vit. D + Ca, Vit. D + Ex, and Vit. D groups compared to the control group; also, inflammatory cell number showed significant increase in the Ex + Vit. D + Ca (12 ± 5.46), Vit. D + Ex (14 ± 3.25), Ex (13 ± 4.08), Vit. D (11 ± 3.26), Ca + Vit. D (10 ± 1.01), and Ca + Ex (9 ± 2.87) groups. Muscle fiber diameter in the Ex + Vit. D + Ca and Vit. D + Ex groups was higher than the other groups. Endomysium thickness was significantly decreased in the Ex + Vit. D + Ca and Vit. D + Ex groups compared to the control and placebo groups (p < 0.001). Degenerative collagen fiber area showed a significant increase in the Ex + Vit. D + Ca and Vit. D + Ex groups (p ≤ 0.001) comparison with the control group.
Conclusion: Regular resistance exercise, Vit. D, and Ca supplements can improve muscle morphological features in the postmenopausal period.
 
 
Keywords: Menopause, Muscle, Vitamin D, Calcium, Exercise.
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Type of Study: Original Article | Subject: Cellular and Molecular Biology of Reproduction
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