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Reproductive Biomedicine
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Volume 19, Issue 7 (July 2021)
IJRM 2021, 19(7): 607-618 |
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Rahimi Asl F, Khosravi M, Hajikhani R, Solati J, Fahimi H. Complementary effects of coenzyme Q10 and Lepidium sativum supplementation on the reproductive function of mice: An experimental study. IJRM 2021; 19 (7) :607-618
URL: http://ijrm.ir/article-1-1963-en.html
URL: http://ijrm.ir/article-1-1963-en.html
1- Department of Biology, Islamic Azad University North Tehran Branch, Tehran, Iran.
2- Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran. ,maryam.khosravi@yahoo.com
3- Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran.
4- Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran.
5- Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2- Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran. ,
3- Department of Biology, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran.
4- Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran.
5- Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Abstract: (1596 Views)
Background: Coenzyme Q10 (CoQ10) and Lepidium sativum (LS) have therapeutic effects on infertility.
Objective: To evaluate the combined effects of LS and CoQ10 on reproductive function in adult male NMRI mice.
Materials and Methods: Eighty three-months-old male mice (35-40 gr) were divided into 4 groups (n = 10/each) of: control (treated with water), CoQ10-treated (200, 300, and 400 mg/kg/body weight), LS-treated (200, 400, 600 mg/kg/body weight), and co-treated (LS [600 mg/kg/body weight] + CoQ10 [200 mg/kg/body weight]) groups. Serum testosterone, luteinizing hormone, follicle-stimulating hormone, and gonadotropin realizing hormone (GnRH) levels were measured using ELISA method. The sperm quality was assessed using Sperm Class Analyzer® (SCA) CASA system and GnRH mRNA expression levels were evaluated by real-time polymerase chain reaction.
Results: The number of sniffing and following behavior was significantly higher in LS-treated (400 and 600 mg/ml/body weight) groups than the control group (p = 0.0007 and p = 0.0010, respectively). The number of mounting and coupling behaviors was significantly higher in the CoQ10 (300 and 400 mg/ml/body weight)-treated animals than the control group (p = 0.0170 and p = 0.0006, respectively). Co-treatment of CoQ10 (200 mg/ml/body weight) and LS (600 mg/ml/body weight) significantly increased all aspects of sexual behaviors as well as the levels of serum testosterone (p = 0.0011), luteinizing hormone (p = 0.0062), and follicle-stimulating hormone (p = 0.0001); sperm viability (p = 0.0300) and motility (p = 0.0010); and GnRH mRNA levels (p = 0.0016) compared to the control group.
Conclusion: The co-administration of CoQ10 and LS significantly improves the activity of the hypothalamic-pituitary-gonadal axis and enhances the reproductive parameters in adult male mice.
Objective: To evaluate the combined effects of LS and CoQ10 on reproductive function in adult male NMRI mice.
Materials and Methods: Eighty three-months-old male mice (35-40 gr) were divided into 4 groups (n = 10/each) of: control (treated with water), CoQ10-treated (200, 300, and 400 mg/kg/body weight), LS-treated (200, 400, 600 mg/kg/body weight), and co-treated (LS [600 mg/kg/body weight] + CoQ10 [200 mg/kg/body weight]) groups. Serum testosterone, luteinizing hormone, follicle-stimulating hormone, and gonadotropin realizing hormone (GnRH) levels were measured using ELISA method. The sperm quality was assessed using Sperm Class Analyzer® (SCA) CASA system and GnRH mRNA expression levels were evaluated by real-time polymerase chain reaction.
Results: The number of sniffing and following behavior was significantly higher in LS-treated (400 and 600 mg/ml/body weight) groups than the control group (p = 0.0007 and p = 0.0010, respectively). The number of mounting and coupling behaviors was significantly higher in the CoQ10 (300 and 400 mg/ml/body weight)-treated animals than the control group (p = 0.0170 and p = 0.0006, respectively). Co-treatment of CoQ10 (200 mg/ml/body weight) and LS (600 mg/ml/body weight) significantly increased all aspects of sexual behaviors as well as the levels of serum testosterone (p = 0.0011), luteinizing hormone (p = 0.0062), and follicle-stimulating hormone (p = 0.0001); sperm viability (p = 0.0300) and motility (p = 0.0010); and GnRH mRNA levels (p = 0.0016) compared to the control group.
Conclusion: The co-administration of CoQ10 and LS significantly improves the activity of the hypothalamic-pituitary-gonadal axis and enhances the reproductive parameters in adult male mice.
Type of Study: Original Article |
Subject:
Reproductive Biology
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