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Volume 20, Issue 8 (August 2022)
IJRM 2022, 20(8): 651-662 |
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Watcho P, Nsamou Ngueyong P C, Defo Deeh P B, Bonsou Fozin G R, Ngadjui E, Wankeu-Nya M et al . Amaranthus hybridus (Amaranthaceae) prevents the detrimental effects of cyclophosphamide on ovarian function in Wistar rats: An experimental study. IJRM 2022; 20 (8) :651-662
URL: http://ijrm.ir/article-1-2036-en.html
URL: http://ijrm.ir/article-1-2036-en.html
Pierre Watcho * 
1, Prechmy Carole Nsamou Ngueyong2 , Patrick Brice Defo Deeh2 , Georges Romeo Bonsou Fozin2 , Esther Ngadjui3 , Modeste Wankeu-Nya4 , Pierre Kamtchouing5
1, Prechmy Carole Nsamou Ngueyong2 , Patrick Brice Defo Deeh2 , Georges Romeo Bonsou Fozin2 , Esther Ngadjui3 , Modeste Wankeu-Nya4 , Pierre Kamtchouing5
1- Research Unit of Animal Physiology and Phytopharmacology (URPAP), Faculty of Science, University of Dschang, Dschang, Cameroon. , pwatcho@yahoo.fr
2- Research Unit of Animal Physiology and Phytopharmacology (URPAP), Faculty of Science, University of Dschang, Dschang, Cameroon.
3- Research Unit of Animal Physiology and Phytopharmacology (URPAP), Faculty of Science, University of Dschang, Dschang, Cameroon. Department of Biomedical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
4- Department of Animal Organisms Biology, University of Douala, Douala, Cameroon.
5- Department of Animal Biology and Physiology, University of Yaoundé 1, Yaoundé, Cameroon.
2- Research Unit of Animal Physiology and Phytopharmacology (URPAP), Faculty of Science, University of Dschang, Dschang, Cameroon.
3- Research Unit of Animal Physiology and Phytopharmacology (URPAP), Faculty of Science, University of Dschang, Dschang, Cameroon. Department of Biomedical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
4- Department of Animal Organisms Biology, University of Douala, Douala, Cameroon.
5- Department of Animal Biology and Physiology, University of Yaoundé 1, Yaoundé, Cameroon.
Abstract: (993 Views)
Background: Cyclophosphamide (CP) is an anticancer agent, but its chronic administration induces ovarian toxicity.
Objective: We evaluated the effects of aqueous extract (AE) and methanol extract (ME) of Amaranthus hybridus (A. hybridus) on CP-induced ovarian toxicity in rats.
Materials and Methods: 40 female Wistar rats (10 wk, 170-200 gr) were distributed into 8 groups (n = 5/each) as follows: 1) healthy control; 2) CP+distilled water (10 ml/kg/d); 3) CP+3%-tween 80 (10 mL/kg/d); 4) CP+clomiphene citrate (2 mg/kg/d); 5, 6) CP+AE of A. hybridus (55 and 110 mg/kg/d); and 7, 8) CP+ME of A. hybridus (55 and 110 mg/kg/d). After 28 days of treatment, estrus cyclicity, ovarian and uterine weights as well as estradiol levels and ovarian histology were determined.
Results: CP induced ovarian toxicity after 28 days of exposure. More specifically, CP disturbed the estrus cycle, decreased ovary and uterus weights (p = 0.04), and the 17-β estradiol level (p = 0.04), and induced severe ovarian damages. Remarkably, A. hybridus significantly increased (p = 0.03) the ovarian weight (AE and ME at all doses) and uterus weight (ME at 110 mg/kg/d), compared with the CP-treated rats. Moreover, the 17-β estradiol level was significantly elevated (p = 0.02) in rats given clomiphene citrate and A. hybridus (AE 110 mg/kg/d; ME 55 mg/kg/d). Finally, the ovaries of rats given plant extracts had many corpus luteum and normal follicles, and no cystic follicles.
Conclusion: A. hybridus prevented the detrimental effects of CP on ovarian function, which could support its traditional use as a fertility enhancer.
Objective: We evaluated the effects of aqueous extract (AE) and methanol extract (ME) of Amaranthus hybridus (A. hybridus) on CP-induced ovarian toxicity in rats.
Materials and Methods: 40 female Wistar rats (10 wk, 170-200 gr) were distributed into 8 groups (n = 5/each) as follows: 1) healthy control; 2) CP+distilled water (10 ml/kg/d); 3) CP+3%-tween 80 (10 mL/kg/d); 4) CP+clomiphene citrate (2 mg/kg/d); 5, 6) CP+AE of A. hybridus (55 and 110 mg/kg/d); and 7, 8) CP+ME of A. hybridus (55 and 110 mg/kg/d). After 28 days of treatment, estrus cyclicity, ovarian and uterine weights as well as estradiol levels and ovarian histology were determined.
Results: CP induced ovarian toxicity after 28 days of exposure. More specifically, CP disturbed the estrus cycle, decreased ovary and uterus weights (p = 0.04), and the 17-β estradiol level (p = 0.04), and induced severe ovarian damages. Remarkably, A. hybridus significantly increased (p = 0.03) the ovarian weight (AE and ME at all doses) and uterus weight (ME at 110 mg/kg/d), compared with the CP-treated rats. Moreover, the 17-β estradiol level was significantly elevated (p = 0.02) in rats given clomiphene citrate and A. hybridus (AE 110 mg/kg/d; ME 55 mg/kg/d). Finally, the ovaries of rats given plant extracts had many corpus luteum and normal follicles, and no cystic follicles.
Conclusion: A. hybridus prevented the detrimental effects of CP on ovarian function, which could support its traditional use as a fertility enhancer.
Type of Study: Original Article |
Subject:
Reproductive Physiology
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