Volume 20, Issue 5 (May 2022)                   IJRM 2022, 20(5): 347-356 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Syahrizal D, Mustika C, Puspita N A, Suryokusumo M G, Hendarto H. The effect of hyperbaric oxygen therapy in the inflammatory response in a mouse model of endometriosis: An experimental study. IJRM 2022; 20 (5) :347-356
URL: http://ijrm.ssu.ac.ir/article-1-2076-en.html
1- Department of Biochemistry, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia. , dedysyahrizal@unsyiah.ac.id
2- Department of Public Health, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia.
3- Department of Biochemistry, Medical Faculty, Universitas Syiah Kuala, Banda Aceh, Indonesia.
4- Medical Hyperbaric Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
5- Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Abstract:   (757 Views)
Background: Endometriosis pathogenesis is related to the inflammation shown by the secretion of pro-inflammatory mediators. This hypoxia condition can stimulate this condition.
Objective: To investigate the effect of hyperbaric oxygen therapy (HBOT) on the inflammation reaction of endometriosis-induced mice.
Materials and Methods: The animals were designated into 3 groups: I) the pre-test group, II) the post-test group receiving the HBOT, and III) the post-test group without HBOT. All groups were subjected to induction of endometriosis by xenotransplantation for 15 days. HBOT was given 30 min 3 times a day for 10 days. The evaluation of the HBOT effect was conducted by examining the endometrial tissue. The inflammation level was evaluated using the Klopfleisch semiquantitative scoring system (index remmele scale), whilst the expression of nuclear factor kappa (NFκB) beta was measured by immunohistochemical staining.
Results: The results showed that group I demonstrated the highest level of inflammation degree (9.41 ± 1.99) compared to the post-test groups (group II: 1.60 ± 0.53; group III: 2.42 ± 0.53). The HBOT-groups was found to have the lowest inflammation level compared to the non-HBOT group (p = 0.020). The results demonstrated that HBOT lowered the peritoneal inflammation degree caused by the endometrial lesion in mice. NFκB expression on the post-test groups was significantly decreased, compared to the pre-test group (p ≤ 0.001), with a strong correlation between the NFκB expression and the peritoneal inflammation level (p ≤ 0.001, r = 0.670).
Conclusion: HBOT significantly reduced the inflammation level on the endometrial lesion in mice, involving the NFκB pathway.
Full-Text [PDF 2053 kb]   (583 Downloads) |   |   Full-Text (HTML)  (108 Views)  
Type of Study: Original Article | Subject: Reproductive Biology

References
1. Laganà AS, Garzon S, Götte M, Vigano P, Franchi M, Ghezzi F, et al. The pathogenesis of endometriosis: Molecular and cell biology insights. Int J Mol Sci 2019; 20: 5615. [DOI:10.3390/ijms20225615] [PMID] [PMCID]
4. García-Gómez E, Vázquez-Martínez ER, Reyes-Mayoral Ch, Cruz-Orozco OP, Camacho-Arroyo I, Cerbón M. Regulation of inflammation pathways and inflammasome by sex steroid hormones in endometriosis. Front Endocrinol 2019; 10: 935. [DOI:10.3389/fendo.2019.00935] [PMID] [PMCID]
7. Wu MH, Lu ChW, Chang FM, Tsai ShJ. Estrogen receptor expression affected by hypoxia inducible factor-1α in stromal cells from patients with endometriosis. Taiwan J Obstet Gynecol 2012; 51: 50-54. [DOI:10.1016/j.tjog.2012.01.010] [PMID]
10. Zhang L, Xiong W, Li N, Liu H, He H, Du Y, et al. Estrogen stabilizes hypoxia-inducible factor 1α through G protein-coupled estrogen receptor 1 in eutopic endometrium of endometriosis. Fertil Steril 2017; 107: 439-447. [DOI:10.1016/j.fertnstert.2016.11.008] [PMID] [PMCID]
13. Sun Y, Wen Y, Shen Ch, Zhu Y, You W, Meng Y, et al. Hyperbaric oxygen therapy in liver diseases. Int J Med Sci 2018; 15: 782-787. [DOI:10.7150/ijms.24755] [PMID] [PMCID]
16. Syahrizal D, Mustika C, Renaldi T, Suryokusumo MG, Hendarto H. Hyperbaric oxygen (HBO) reduce expression of hypoxia inducible factor-1 Alpha (HIF-1 alpha) and endometriotic tissue size in mice model of endometriosis. Paper presented at: The 1St International Conference on Veterinary, Animal, and Environmental Sciences; 2019 October 15-17; Indonesia. [DOI:10.1051/e3sconf/202015101003]
18. Sutrisno S, Sri A, Wiyasa IWA, Umi K, Noerhamdani N, Hidayat S, et al. The effect of implant origin differences on peritoneal endometriosis in an endometriosis mouse model. Int J Women's Health Reprod Sci 2019; 7: 34-40. [DOI:10.15296/ijwhr.2019.06]
20. Klopfleisch R. Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology: A systematic review. BMC Vet Res 2013; 9: 123. [DOI:10.1186/1746-6148-9-123] [PMID] [PMCID]
23. Han Z, Boyle DL, Manning AM, Firestein GS. AP-1 and NF-kB regulation in rheumatoid arthritis and murine collagen-induced arthritis. Autoimmunity 1998; 28: 197-208. [DOI:10.3109/08916939808995367] [PMID]
26. Nowak M, Madej JA, Dziegiel P. Intensity of COX2 expression in cells of soft tissue fibrosacrcomas in dogs as related to grade of tumour malignancy. Bullet Vet Institute Pulawy 2007; 51: 275-279.
27. Carney AY. Hyperbaric oxygen therapy: An introduction. Crit Care Nurs Q 2013; 36: 274-279. [DOI:10.1097/CNQ.0b013e318294e936] [PMID]
30. Shinomiya N. Molecular mechanisms of hyperbaric oxygen therapy. In: Shinomiya N, Asai Y. Hyperbaric oxygenation therapy. Singapore: Springer; 2020: 3-20. [DOI:10.1007/978-981-13-7836-2_1]
32. Lam G, Fontaine R, Ross FL, Chiu ES. Hyperbaric oxygen therapy: Exploring the clinical evidence. Adv Skin Wound Care 2017; 30: 181-190. [DOI:10.1097/01.ASW.0000513089.75457.22] [PMID]
35. Oley MH, Oley MCh, Islam AA, Hatta M, Faruk M, Noersasongko AD, et al. Hyperbaric oxygen therapy in managing systemic inflammatory response syndrome caused by ischemia-reperfusion injury following hand replantation and long-term outcomes: A report of two cases. Ann Med Surg 2020; 60: 155-161. [DOI:10.1016/j.amsu.2020.10.023] [PMID] [PMCID]
38. Li C, Zhao HL, Li YJ, Zhang YY, Liu HY, Feng FZh, et al. The expression and significance of leukemia inhibitory factor, interleukin-6 and vascular endothelial growth factor in Chinese patients with endometriosis. Arch Gynecol Obstet 2021; 304: 163-170. [DOI:10.1007/s00404-021-05980-5] [PMID]
41. Kaponis A, Iwabe T, Taniguchi F, Ito M, Deura I, Decavalas G, et al. The role of NF-kappaB in endometriosis. Front Biosci 2012; 4: 1213-1234. [DOI:10.2741/s327] [PMID]
44. Hsiao KY, Lin ShCh, Wu MH, Tsai ShJ. Pathological functions of hypoxia in endometriosis. Front Biosci 2015; 7: 309-321. [DOI:10.2741/e736] [PMID]
47. Eltzschig HK, Carmeliet P. Hypoxia and inflammation. N Engl J Med 2011; 364: 656-665. [DOI:10.1056/NEJMra0910283] [PMID] [PMCID]
50. Aydin Y, Atis A, Uludag S, Tezer I, Sakiz D, Acar H, et al. Remission of endometriosis by hyperbaric oxygen treatment in rats. Reprod Sci 2011; 18: 941-947. [DOI:10.1177/1933719111400635] [PMID]
53. Yu X, Li YG, He XW, Li XR, Din BN, Gan Y, et al. Hyperbaric oxygen reduces inflammatory response in acute pancreatitis by inhibiting NF-κB activation. Eur Surg Res 2009; 42: 130-135. [DOI:10.1159/000196164] [PMID]
56. Velnar T, Bailey T, Smrkolj V. The wound healing process: An overview of the cellular and molecular mechanisms. J Int Med Res 2009; 37: 1528-1542. [DOI:10.1177/147323000903700531] [PMID]
59. Benson RM, Minter LM, Osborne BA, Granowitz EV. Hyperbaric oxygen inhibits stimulus‐induced proinflammatory cytokine synthesis by human blood‐derived monocyte‐macrophages. Clin Exp Immunol 2003; 134: 57-62. [DOI:10.1046/j.1365-2249.2003.02248.x] [PMID] [PMCID]
62. Yang Z, Nandi J, Wang J, Bosco G, Gregory M, Chung C, et al. Hyperbaric oxygenation ameliorates indomethacin-induced enteropathy in rats by modulating TNF-α and IL-1β production. Dig Dis Sci 2006; 51: 1426-1433. [DOI:10.1007/s10620-006-9088-2] [PMID]

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb