International Journal of
Reproductive Biomedicine
Sat, Aug 13, 2022
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Volume 20, Issue 7 (July 2022)
IJRM 2022, 20(7): 561-568 |
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Barzanouni S, Moramezi F, Zargar M, Galehdari H, Hemadi M. A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium. IJRM. 2022; 20 (7) :561-568
URL: http://ijrm.ssu.ac.ir/article-1-2120-en.html
URL: http://ijrm.ssu.ac.ir/article-1-2120-en.html
1- Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , drbarzanonis@gmail.com
2- Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Genetics, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
5- Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2- Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Department of Obstetrics and Gynecology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Genetics, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
5- Fertility, Infertility and Perinatology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Abstract: (102 Views)
Background: Preimplantation genetic diagnosis (PGD) has been used as an option for couples with the possibility of having a baby with a genetic disorder. The common method for performing this test involves isolating one cell from day 3 or a few cells from day 5 embryos and performing genetic studies on the cell-extracted DNA. This method is invasive and can cause abortion after implantation in the uterus. Because of this, 2 noninvasive methods for performing a PGD have been studied :PGD using blastocyst fluid and PGD using embryo culture medium.
Objective: The aim of this study is to determine the sensitivity of polymerase chain reaction (PCR) technique to detect Y chromosome using cell-free DNA within culture medium for gender prediction of blastocysts.
Materials and Methods: In this study, the gender of 30 embryos on day 5 was determined using embryonic DNA extraction from the culture medium, and the PCR technique to evaluate genes sex-determining Region Y and fragile X mental retardation. Then, their accuracy was assessed using ultrasound.
Results: The results of the PCR technique showed that 7 embryos were male, but an ultrasound revealed that 13 were male.
Conclusion: The given results indicated that because of the low amount of DNA extracted from the culture medium, the diagnosis of the existence of the Y chromosome by this method is still not accurate enough for detecting the gender of the embryo.
Objective: The aim of this study is to determine the sensitivity of polymerase chain reaction (PCR) technique to detect Y chromosome using cell-free DNA within culture medium for gender prediction of blastocysts.
Materials and Methods: In this study, the gender of 30 embryos on day 5 was determined using embryonic DNA extraction from the culture medium, and the PCR technique to evaluate genes sex-determining Region Y and fragile X mental retardation. Then, their accuracy was assessed using ultrasound.
Results: The results of the PCR technique showed that 7 embryos were male, but an ultrasound revealed that 13 were male.
Conclusion: The given results indicated that because of the low amount of DNA extracted from the culture medium, the diagnosis of the existence of the Y chromosome by this method is still not accurate enough for detecting the gender of the embryo.
Keywords: Preimplantation diagnosis, Embryo implantation, Culture media, Blastocyst, Polymerase chain reaction.
Type of Study: Original Article |
Subject:
Perinatology
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