Volume 20, Issue 5 (May 2022)                   IJRM 2022, 20(5): 399-404 | Back to browse issues page

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Arefnia M, Motovali-Bashi M, Javadirad S, Norioun H. Overexpression of hsa-miR-30a-5p and non-obstructive azoospermia: A case-control study. IJRM 2022; 20 (5) :399-404
URL: http://ijrm.ir/article-1-2234-en.html
Overexpression of hsa-miR-30a-5p and non-obstructive azoospermia: A case-control study
Mohammad Arefnia1 , Majid Motovali-Bashi * 2, Seyed-Morteza Javadirad1 , Hamid Norioun3
1- Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
2- Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran. , mbashi@sci.ui.ac.ir
3- Medical Genetics Department, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Abstract:   (1307 Views)
Background: Some previous human and animal studies have supported the idea that KDM3A down-regulation might be the main cause of male infertility, especially in non-obstructive azoospermia (NOA). The regulatory role of micro-RNAs (miRNA) has been investigated in the development of male infertility.
Objective: The expression level of hsa-miR-30a-5p in azoospermia was evaluated to reveal its possible association with the etiology of male infertility.
Materials and Methods: In this case-control study, 30 men with azoospermia (19 of whom had NOA) were selected as the case individuals, and 11 men with obstructive azoospermia (OA) were selected as control individuals. The best miRNA with the strongest ability to target the KDM3A gene was detected via comprehensive bioinformatics analysis. Reverse transcriptase quantitative polymerase chain reaction was used to assess the expression level of hsa-miR-30a-5p. After analyzing the data, the expression level of hsa-miR-30a-5p was compared between men with NOA and men with OA.
Results: The findings supported the idea that hsa-miR-30a-5p is the miRNA with the best ability to target the KDM3A transcript. The expression analysis of hsa-miR-30a-5p indicated a significant overexpression (p = 0.04) in men with NOA compared to in men with OA.
Conclusion: Hsa-miR-30a-5p was overexpressed in men with NOA compared to in control individuals. Hsa-miR-30a-5p could target the KDM3A transcript and may suppress its expression.
Keywords: Hsa-miR-30a-5p, Male infertility, KDM3A, Azoospermia, miRNA.
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Type of Study: Original Article | Subject: Reproductive Genetics
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