International Journal of
Reproductive Biomedicine
Fri, Apr 19, 2024
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Volume 21, Issue 1 (January 2023)
IJRM 2023, 21(1): 61-70 |
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AL-ghamdi M, Huwait E, Elsawi N, Shaker Ali S, Sayed A. Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study. IJRM 2023; 21 (1) :61-70
URL: http://ijrm.ir/article-1-2259-en.html
URL: http://ijrm.ir/article-1-2259-en.html
1- Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah, Saudi Arabia. Experimental Biochemistry Unit, King Fahad Medical Research Centre, King AbdulAziz University, Jeddah, Saudi Arabia. Vitamin D Pharmacogenomics Research Group, King AbdulAziz University, Saudi Arabia.
2- Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah, Saudi Arabia. Experimental Biochemistry Unit, King Fahad Medical Research Centre, King AbdulAziz University, Jeddah, Saudi Arabia.
3- Department of Chemistry, Lab Biochemistry, Faculty of Science, Sohag University, Sohag, Egypt.
4- Faculty of Medicine, Merit University, Sohag, Egypt.
5- Biochemistry Laboratory, Faculty of Science, Chemistry Department, Assiut University, Assiut, Egypt. , ahmed.sayed@aun.edu.eg
2- Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah, Saudi Arabia. Experimental Biochemistry Unit, King Fahad Medical Research Centre, King AbdulAziz University, Jeddah, Saudi Arabia.
3- Department of Chemistry, Lab Biochemistry, Faculty of Science, Sohag University, Sohag, Egypt.
4- Faculty of Medicine, Merit University, Sohag, Egypt.
5- Biochemistry Laboratory, Faculty of Science, Chemistry Department, Assiut University, Assiut, Egypt. , ahmed.sayed@aun.edu.eg
Abstract: (723 Views)
Background: Acrylamide (AA) is a carcinogenic compound that causes severe reproductive impairments and represents a high environmental risk factor. Thymoquinone (TQ) has a unique antioxidant activity and has been widely used as a protective agent against various types of toxicity.
Objective: To evaluate the protective effects of TQ against AA-induced reproductive toxicity in female rats.
Materials and Methods: In this experimental study, 40 female albino rats (120-150 gr, 8-10 wk) were sorted into 4 groups, (n = 10/each), vehicle group (received a daily oral administration of 0.5 ml saline [9%]); AA group (received a daily oral administration with freshly prepared AA, 20 mg/kg body weight) for 21 days which is less than the lethal dose LD50 of AA in rats (20 mg /kg body weight); AA+TQ group (received a daily oral administration of TQ, 10 mg/kg body weight) after AA intoxication for 21 days, and TQ group (received a daily oral administration of TQ only, 10 mg/kg body weight) for 21 consecutive days. Reproductive hormones, carcinogenic biomarkers, and oxidative stress markers were measured. The histological assessment showed the protective effect of TQ against AA-induced ovarian injury. Network pharmacology analysis and molecular docking approach were carried out to determine the binding affinity of TQ with cyclooxygenase 2.
Results: TQ administration significantly enhanced the functional capacity of the ovary at hormones, oxidative biomarkers, and tumor markers at a significant level of p < 0.001. Besides, TQ protects the ovary of AA-treated rats from the severe degeneration effect.
Conclusion: TQ showed a promising protective effect against AA-induced reproductive toxicity in female rats.
Objective: To evaluate the protective effects of TQ against AA-induced reproductive toxicity in female rats.
Materials and Methods: In this experimental study, 40 female albino rats (120-150 gr, 8-10 wk) were sorted into 4 groups, (n = 10/each), vehicle group (received a daily oral administration of 0.5 ml saline [9%]); AA group (received a daily oral administration with freshly prepared AA, 20 mg/kg body weight) for 21 days which is less than the lethal dose LD50 of AA in rats (20 mg /kg body weight); AA+TQ group (received a daily oral administration of TQ, 10 mg/kg body weight) after AA intoxication for 21 days, and TQ group (received a daily oral administration of TQ only, 10 mg/kg body weight) for 21 consecutive days. Reproductive hormones, carcinogenic biomarkers, and oxidative stress markers were measured. The histological assessment showed the protective effect of TQ against AA-induced ovarian injury. Network pharmacology analysis and molecular docking approach were carried out to determine the binding affinity of TQ with cyclooxygenase 2.
Results: TQ administration significantly enhanced the functional capacity of the ovary at hormones, oxidative biomarkers, and tumor markers at a significant level of p < 0.001. Besides, TQ protects the ovary of AA-treated rats from the severe degeneration effect.
Conclusion: TQ showed a promising protective effect against AA-induced reproductive toxicity in female rats.
Type of Study: Original Article |
Subject:
Reproductive Biology
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