Volume 9, Issue 3 (7-2011)                   IJRM 2011, 9(3): 203-208 | Back to browse issues page

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Zhang X, Yang Y, Min L, Lv Q, Bai P, Li X et al . Successful pregnancy following the transfer of vitrified blastocyst which developed from poor quality embryos on day 3. IJRM 2011; 9 (3) :203-208
URL: http://ijrm.ir/article-1-228-en.html
Successful pregnancy following the transfer of vitrified blastocyst which developed from poor quality embryos on day 3
Xiao-jian Zhang * 1, Ye-zhou Yang2 , Li-hua Min2 , Qun Lv2 , Pin Bai2 , Xiao-jie Li2 , Mei-xu Liao2
1- Department of Assisted Reproductive Medical Center, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Sichuan, China , xiaojian.z@gmail.com
2- Department of Assisted Reproductive Medical Center, Sichuan Academy of Medical Sciences and Sichuan Provincial People’s Hospital, Sichuan, China
Abstract:   (2947 Views)
Background: The selection of pre-embryos for transferred is based on morphological appearance. But some poor quality cleaved embryos also can be cultured to the blastocyst stage and implanted.
Objective: To assess the clinical pregnancy outcomes of blastocyst transfer which developed from poor quality embryos.
Materials and Methods: A total of 109 cleaved embryos with poor quality were cultured to day 5/day 6 and 27 (24.8%) blastocysts were collected from the 15 cycles/patients undergoing conventional IVF. All the blastocysts were cooling with fast-freezing. Then the blastocysts were warmed for transfer. Results: All of 25 vitrified blastocysts (92.6%) survived after warming and were transferred to 15 patients. Five of the women became pregnant.
Conclusion: Our results suggest that vitrified human day 5/day 6 blastocyst transfer which develop from poor quality embryo at day 3 can contribute to increasing cumulative pregnancy rates in assisted reproduction.
Keywords: Poor quality embryos, Vitified Blastocyst- transfer, Pregnancy.
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Type of Study: Original Article |
References
1. Farhi J, Nahum H, Weissman A, Roybur M, Glezerman M, Levran D. The value of culturing to the blastocyst stage of supernumerary embryos not suitable for freezing after embryo transfer at day 3. Fertil Steril 2001; 76 (Suppl.): 118. [DOI:10.1016/S0015-0282(01)02347-0]
2. Hardarson T, Hanson C, Sjögren A, Lundin K. Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod 2001; 16: 313-318. [DOI:10.1093/humrep/16.2.313]
3. Rijnders PM, Jansen CA. The predictive value of day 3 embryo morphology regarding blastocyst formation, pregnancy and implantation rate after day 5 transfer following in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod 1998; 13: 2869-2873. [DOI:10.1093/humrep/13.10.2869]
4. Balaban B, Urman B, Alatas C, Mercan R, Aksoy S, Isiklar A. Blastocyst-stage transfer of poor-quality cleavage-stage embryos results in higher implantation rates. Fertil Steril 2001; 75: 514-518. [DOI:10.1016/S0015-0282(00)01756-8]
5. Hiraoka K, Hiraoka K, Kinutani M, Kinutani K. Vitrification of human hatched blastocysts: a report of 4 cases. J Reprod Med 2007; 52: 413-415.
6. Zhang XJ, Yang YZ, Lv Q, Min LH, Li XJ, Bai P. Effect of the size of zona pellucida thinning by laser assisted hatching on clinical outcome of human frozen-thawed embryo transfers. Cryo Letters 2009; 30: 455-461.
7. Hiraoka K, Fujimoto Y, Tateaki Y, Hiraoka K, Horiuchi T, Okano S, et al. Case report: two successful pregnancies following the transfer of re-vitrified human day 7 blastocysts developed from vitrified cleaved embryos. J Assist Reprod Genet 2008; 25: 503-509. [DOI:10.1007/s10815-008-9259-5]
8. Graham J, Han T, Porter R, Levy M, Stillman R, Tucker MJ. Day 3 morphology is a poor predictor of blastocyst quality in extended culture. Fertil Steril 2000; 74: 495-497. [DOI:10.1016/S0015-0282(00)00689-0]
9. Braude P, Bolton V, Moore S. Human gene expression first occurs between the 4-cell and 8-cell stages of preimplantation development. Nature 1988; 332: 459-461. [DOI:10.1038/332459a0]
10. Van Royen E, Mangelschots K, De Neubourg D, Valkenburg M, Van de meerssche M, Ryckaert G, et al. Characterization of a top quality embryo, a step towards single-embryo transfer. Hum Reprod 1999; 14: 2345-2349. [DOI:10.1093/humrep/14.9.2345]
11. Liu W, Yin Y, Long X, Luo Y, Jiang Y, Zhang W, et al. Derivation and characterization of human embryonic stem cell lines from poor quality embryos. J Genet Genomics 2009; 36: 229-239. [DOI:10.1016/S1673-8527(08)60110-1]
12. Raya A, Rodríguez-Pizà I, Arán B, Consiglio A, Barri PN, Veiga A, et al. Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts. Cold Spring Harb Symp Quant Biol 2008; 73: 127-135. [DOI:10.1101/sqb.2008.73.038]
13. Ménézo YJ. Blastocyst freezing. Eur J Obstet Gynecol Reprod Biol 2004; 115 (Suppl.): 12-15. [DOI:10.1016/j.ejogrb.2004.01.007]
14. Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J, Hesla J. A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 1998; 13: 3434-3440. [DOI:10.1093/humrep/13.12.3434]
15. Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, et al. Successful pregnancy following blastocyst vitrification: case report. Hum Reprod 2000; 15: 1802-1803. [DOI:10.1093/humrep/15.8.1802]
16. Hiraoka K, Hiraoka K, Horiuchi T, Kusuda T, Okano S, Kinutani M, et al. Case report: successful delivery following the transfer of a human re-vitrified day-7 spontaneously hatched blastocyst developed from vitrified cleaved embryos. J Assist Reprod Genet 2009; 26: 405-409. [DOI:10.1007/s10815-009-9333-7]
17. Zhang JQ, Li XL, Peng Y, Guo X, Heng BC, Tong GQ. Reduction in exposure of human embryos outside the incubator enhances embryo quality and blastulation rate. Reprod Biomed Online 2010; 20: 510-515. [DOI:10.1016/j.rbmo.2009.12.027]
18. Van Landuyt L, De Vos A, Joris H, Verheyen G, Devroey P, Van Steirteghem A. Blastocyst formation in in vitro fertilization versus intracytoplasmic sperm injection cycles: influence of the fertilization procedure. Fertil Steril 2005; 83: 1397-1403. [DOI:10.1016/j.fertnstert.2004.10.054]
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