Volume 9, Issue 3 (7-2011)                   IJRM 2011, 9(3): 217-222 | Back to browse issues page

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Hyacinth A A, Nwocha U C. Antifertility activity of aqueous ethanolic extract of Hymenocardia acida stem bark in female rats. IJRM 2011; 9 (3) :217-222
URL: http://ijrm.ir/article-1-230-en.html
Antifertility activity of aqueous ethanolic extract of Hymenocardia acida stem bark in female rats
Abu Adakole Hyacinth * 1, Uchendu Chukwuka Nwocha2
1- Department of Veterinary Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Agriculture, P.M.B, 2373, Makurdi, Nigeria , adakoleabu@yahoo.co.uk
2- Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
Abstract:   (2479 Views)
Background: Hymenocardia acida is traditionally used in African herbal medicine and has numerous therapeutic benefits. But little is known about its potentially negative effects on pregnant women.
Objective: The aim of the present study was to evaluate the antifertility effect of aqueous ethanolic extract of Hymenocardia acida stem bark in female Wistar rats.
Materials and Methods: Four groups of rats were administered orally aqueous ethanolic extract of Hymenocardia acida at doses of 100, 200 and 400 mg/kg body weight daily for 19 days. The control group received distilled water. On day 20 of gestation, each rat was laparatomised and number of corpora lutea of pregnancy, number of live fetuses as well as the postcoitum fertility index, weights of the foetuses and placentae were determined. Results: Oral administration of the extract from days 1 to 19 of gestation showed reduction (p<0.05) in the number of corpora lutea of pregnancy and number of live fetuses. Weights of fetuses of extract treated female rats were also smaller (p<0.05) compared with the control. Anti-implantation activity of the treatment groups were 41.4%, 48.3% and 51.7% for groups II to IV respectively, whereas antifertility activity of the groups was found to be 40%, 60% and 60% in the same order.
Conclusion: The results suggest that aqueous ethanolic extract of Hymenocardia acida stem bark could induce negative effects on reproductive functions in female albino rats.
Keywords: Albino rats, Antifertility, Hymenocardia acida, Reproduction.
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Type of Study: Original Article |
References
1. Uchendu CN, Kamalu TN, Asuzu IU. A preliminary evaluation of antifertility activity of a triterpenoid glycoside from Dalbergia saxatilis in female Wistar rats. Pharmacol Res 2000; 41:521-525. [DOI:10.1006/phrs.1999.0615]
2. Srivastava S, Keshri G, Bhargevan B, Singh C, Singh MM. Pregnancy interceptive activity of the roots of Calotropis gigantea Linn in rats. Contraception 2007; 75: 318-322. [DOI:10.1016/j.contraception.2006.11.010]
3. Muller JC, Botelho GG, Bufalo AC, Boaereto AC, Rattman YD, Martins ES, et al. Morinda citrifolia Linn (Noni): In vivo and in vitro reproductive toxicology. J Ethnopharmacol 2009; 121: 229-233. [DOI:10.1016/j.jep.2008.10.019]
4. Costa-Silva JH, Lima CR, Silva EJ, Araujo AV, Fraga MC, Ribeiro E Ribeiro A, et al. Acute and sub acute toxicity of the Carapa guianensis Aublet (Meliaceae) seed oil. J Ethnopharmacol 2008; 116: 495-500. [DOI:10.1016/j.jep.2007.12.016]
5. Muanza DN, Euler KL, Williams L, Newman DS. Screening for antitumor and anti-HIV activities of nine medicinal plants from Zaire. Int J Pharmacol 1995; 33: 98-106. [DOI:10.3109/13880209509055207]
6. Mpiana P T, Tshibanga DS, Shetonde OM, Ngbolua KN. In vitro antidrepanocytary activity (antisickle cell anaemia) of some Congolese plants. Phytomedicine 2007; 14: 192-195. [DOI:10.1016/j.phymed.2006.05.008]
7. Ukwe CV. Evaluation of the anti-ulcer activity of aqueous stem bark extract of Hymenocardia acida. Nigerian Journal of Pharmaceutical Research 2004; 3: 86-89.
8. Tona L, Kambu K, Masia K, Cimanga R, Apers S, De bruyne T, et al. Biological screening of traditional preparations from some medicinal plants used as antidiarrhoeal in Kinshasha, Congo. Phytomedicine 1999; 6: 59-66. [DOI:10.1016/S0944-7113(99)80036-1]
9. Igoli JO, Ogeji OG, Tor-Anyiin TA, Igoli NP. Traditional medical practice amongst the igede people of Nigeria. Afr J Trad CAM 2005; 2: 134-152.
10. Hoet S, Opperdoes F, Brun R, Adjakidje V, Quetinleclercq J. In vitro antitrypanosomal activity of ethno pharmacologically selected Beninese plants. J Ethnopharmacol 2004; 91: 37-42. [DOI:10.1016/j.jep.2003.11.008]
11. Kamanzi Atindehook K, Schmid C, Brun R, Kone MW, Traore D. Antitrypanosomal and antiplasmodial activity of medicinal plants from Cote d'Ivoire. J Ethnopharmacol 2004; 90: 221-227. [DOI:10.1016/j.jep.2003.09.032]
12. Vonthron-Senecheau C, Weniger B, Quattara M, Bi FT, Kamenan A, Lobstein A. In vitro antiplasmodial activity and cytotoxicity of ethno botanically selected Ivorian plants. J Ethnopharmacol 2003; 87: 221-225. [DOI:10.1016/S0378-8741(03)00144-2]
13. Abu AH, Uchendu CN, Ofukwu RA. In vitro antitrypanosomal activity of crude extracts of some Nigerian medicinal plants. J Appl Biosc 2009; 21: 1277-1282.
14. National Institute of Health (N.I.H). Guide for the care and use of laboratory animals. DHEW Publication. Office of Science and Health Reports; Bethesda; U.S.A.; 1985.
15. Wong PY, Lau SK, Fu WO. Antifertility effects of some sulphonamides and related compounds and their accumulation in the rat epididymis. J Reprod Fertil 1987; 81: 259-267. [DOI:10.1530/jrf.0.0810259]
16. Tafessel G, Mekonnen Y, Makonnen E. In vivo and in vitro antifertility and antiimplantation properties of Leonotis ocymifolia in rats. Afr J Trad CAM 2005; 2: 103- 112.
17. Uchendu CN, Isek T. Antifertility activity of aqueous ethanolic leaf extract of Spondias mombin (Anacardiaceae) in rats. Afr Health Sci 2008; 8: 163-167.
18. Yadav R, Jain GC. Antifertility effect and hormonal profile of petroleum ether extract of seeds of Cassia fistula in female rats. International Journal of Pharmacology and Technology Research 2009; 1: 438-444.
19. Hikim AP, Lue YH, Wang C, Reutrakul V, Sangsuwan R, Swerdloff RS. Post testicular antifertility action of triptolide in the male rat: evidence for severe impairment of cauda epididymal sperm ultra structure. J Androl 2000; 21: 431-437.
20. Almeida FC, Lemonica IP. The toxic effects of Coleus barbatus on the different periods of pregnancy in rats. J Ethnopharmacol 2000; 73: 53-60. [DOI:10.1016/S0378-8741(00)00275-0]
21. Tafessel G, Mekonnen Y, Makonnen E. Antifertility effect of aqueous and ethanol extracts of the leaves and roots of Asparagus africanus in rats. Afr Health Sci 2006; 6: 81-85.
22. Chang CV, Felicio AC, Reis JE, Guerra MdE O, Peters VM. Fetal toxicity of Solanum lycocarpum (Solanaceae) in rats. J Ethnopharmacol 2008; 81: 205-209.
23. Uguru MO, Okwuasaba FK, Ekwenchi EE, Uguru VE. Uterotonic properties of the methanolic extract of Monechma ciliatum. J Ethnopharmacol 1998; 62: 203-208. [DOI:10.1016/S0378-8741(98)00037-3]
24. Ayinde BA, Onwukaeme DN, Nworgu ZAM. Oxytocic effects of the water extract of Musanga cecropioides R. Brown (Moraceae) stem bark. Afr J Biotechnol 2006; 5: 1350-1354.
25. Singh MM, Kamboj VP. Fetal resorption in rats treated with an antiestrogen in relation to luteal phase nidatory estrogen secretion. Acta Endocrinol (Copenh) 1992; 26: 444-450. [DOI:10.1530/acta.0.1260444]
26. Emiliani S, Delbaere A, Devreker F, Englert Y. Embryo-maternal interactive factors regulating the implantation process: Implications in assisted reproduction. Reprod Biomed Online 2005; 10: 527-540. [DOI:10.1016/S1472-6483(10)60831-0]
27. Hafez B, Hafez ESE. Reproduction in Farm Animals. 7th Ed. Lippincott; Williams and Wilkins; 2000: 3-95. [DOI:10.1002/9781119265306]
28. Orihuela PA, Rios M, Croxatto HB. Disparate effects of estradiol on egg transport and oviductal protein synthesis in mated and cyclic rats. Biol Reprod 2001; 65: 1232-1237. [DOI:10.1095/biolreprod65.4.1232]
29. Vasudeva N, Sharma SK. Estrogenic and pregnancy interceptory effects of Achyranthes aspera Linn root. Afr J Trad, Complement Altern Med 2007; 4: 7-11.
30. Hazarika A, Sarma HN. Effects of crude root extract of Polygonum hydropiper on estrous cycle and induction of reversible sterility in female albino rat. Journal of Endocrinology and Reproduction 2007; 11: 36-40.
31. Rasheed RA, Bashir AK, Ali BH, Padmanabhan R. Effect of Rhazya stricta on the developing rat fetus. Reprod Toxicol 1997; 11: 191-199. [DOI:10.1016/S0890-6238(97)00006-3]
32. Schwarz A, Pinto E, Haraguchi M, de Oliveira CA, Bernardi MM, Spinosa HS. Phytochemical study of Solanum lycocarpum unripe fruit and its effects on rat gestation. Phytotherapy Research 2007; 21: 1025-1028. [DOI:10.1002/ptr.2200]
33. Nana P, Asongalem ER, Foyet HS, Folefoe GN, Dimo T, Kamtchouing P. Maternal and developmental toxicity evaluation of Acanthus montanus leaves extract administered orally to Wistar pregnant rats during organogenesis. J Ethnopharmacol 2008; 116: 228-233. [DOI:10.1016/j.jep.2007.11.021]
34. Boeke SJ, Boersma MG, Alink GM, Van loon JJA, VanHuis A, Dicke M, et al. Safety evaluation of neem (Azadirchta indica) derived pesticides. J Ethnopharmacol 2004; 94: 25-41. [DOI:10.1016/j.jep.2004.05.011]
35. Iranloye B, Owokunle B. Effect of Garcinia kola seed extract on female reproductive functions in rats. Int J Pharmacol 2008; 4: 276-281. [DOI:10.3923/ijp.2008.276.281]
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