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Reproductive Biomedicine
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Volume 9, Issue 3 (7-2011)
IJRM 2011, 9(3): 193-198 |
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Mehdizadeh A, Rahimipour A, Farzadi L, Darabi M, Shahnazi V, Shaaker M, et al . Correlation between the level of cholesteryl ester transfer protein in follicular fluid with fertilization rates in IVF/ ICSI cycles. IJRM 2011; 9 (3) :193-198
URL: http://ijrm.ir/article-1-236-en.html
URL: http://ijrm.ir/article-1-236-en.html
Amir Mehdizadeh * 
1, Ali Rahimipour2 , Laya Farzadi3 , Masoud Darabi4 , Vahideh Shahnazi3 , Maghsod Shaaker4 , Amir-Mansour Vatankhah5 , Zahra Golmohamadi4 , Mohammad Nouri4
1, Ali Rahimipour2 , Laya Farzadi3 , Masoud Darabi4 , Vahideh Shahnazi3 , Maghsod Shaaker4 , Amir-Mansour Vatankhah5 , Zahra Golmohamadi4 , Mohammad Nouri4
1- Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Laboratory Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
4- Laboratory of Chromatography, Department of Biochemistry and clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Laboratory Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
4- Laboratory of Chromatography, Department of Biochemistry and clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract: (2436 Views)
Background: Follicular fluid (FF) plays an important role in oocytes and embryo development, which may contribute to IVF/ICSI success rate.
Objective: The aim of this study was to investigate the correlation between cholesteryl ester transfer protein (CETP) level in FF and the success rate of IVF/ICSI.
Materials and Methods: In a cross-sectional study, FF samples, FF samples were obtained from 100 patients referred to Tabriz Alzahra Hospital. Seventy-nine subjects underwent IVF and the remaining 21 underwent ICSI. The levels of high-density lipoprotein cholesterol (HDL-C), apolipoprotein A-I and CETP were measured using enzymatic, turbidometric and ELISA methods respectively.
Results: Analysis of the subgroups with different levels of CETP showed a significant lower level of CETP in the subgroup with the lowest number of mature oocytes (p<0.05). The level of CETP was also considerably lower (18%, p=0.05) in subjects with<50% oocytes fertilization ratio than subjects with >70% of this ratio.
Conclusion: While no association was found for pregnancy, the amount of CETP in FF was associated positively to the maturity and the percentage of oocyte fertilization.
Objective: The aim of this study was to investigate the correlation between cholesteryl ester transfer protein (CETP) level in FF and the success rate of IVF/ICSI.
Materials and Methods: In a cross-sectional study, FF samples, FF samples were obtained from 100 patients referred to Tabriz Alzahra Hospital. Seventy-nine subjects underwent IVF and the remaining 21 underwent ICSI. The levels of high-density lipoprotein cholesterol (HDL-C), apolipoprotein A-I and CETP were measured using enzymatic, turbidometric and ELISA methods respectively.
Results: Analysis of the subgroups with different levels of CETP showed a significant lower level of CETP in the subgroup with the lowest number of mature oocytes (p<0.05). The level of CETP was also considerably lower (18%, p=0.05) in subjects with<50% oocytes fertilization ratio than subjects with >70% of this ratio.
Conclusion: While no association was found for pregnancy, the amount of CETP in FF was associated positively to the maturity and the percentage of oocyte fertilization.
Type of Study: Original Article |
References
1. Kallen B. Maternal morbidity and mortality in in-vitro fertilization. Best Pract Res Clin Obstet Gynaecol 2008; 22: 549-558. [DOI:10.1016/j.bpobgyn.2008.02.001]
2. Youssry M, Ozmen B, Orief Y, Zohni KH, Al-Hassani S. Human sperm DNA damage in the context of assisted reproductive techniques. Iran J Rep Med 2007; 4: 137-150.
3. Tokumura A, Miyake M, Nishioka Y, Yamano S, Aono T, Fukuzawa K. Production of lysophosphatidic acids by lysophospholipase D in human follicular fluids of In vitro fertilization patients. Biol Reprod 1999; 61:195-199. [DOI:10.1095/biolreprod61.1.195]
4. Grummer RR, Carroll DJ. A review of lipoprotein cholesterol metabolism: importance to ovarian function. J Anim Sci 1988; 66: 3160-3173. [DOI:10.2527/jas1988.66123160x]
5. Perret BP, Parinaud J, Ribbes H, Moatti JP, Pontonnier G, Chap H, et al. Lipoprotein and phospholipid distribution in human follicular fluids. Fertil Steril 1985; 43: 405-409. [DOI:10.1016/S0015-0282(16)48440-2]
6. Shalgi R, Kraicer P, Rimon A, Pinto M, Soferman N. Proteins of human follicular fluid: the blood-follicle barrier. Fertil Steril 1973; 24: 429-434. [DOI:10.1016/S0015-0282(16)39730-8]
7. Jaspard B, Fournier N, Vieitez G, Atger V, Barbaras R, Vieu C, et al. Structural and functional comparison of HDL from homologous human plasma and follicular fluid. A model for extravascular fluid. Arterioscler Thromb Vasc Biol 1997; 17: 1605-1613. [DOI:10.1161/01.ATV.17.8.1605]
8. Ghatrehsamani K, Darabi M, Rahbani M, Hashemzadeh Chaleshtory M, Farrokhi E, Noori M. Combined hepatic lipase -514C/T and cholesteryl ester transfer protein I405V polymorphisms are associated with the risk of coronary artery disease. Genet Test Mol Biomarkers 2009; 13: 809-815. [DOI:10.1089/gtmb.2009.0080]
9. Morton RE. Interaction of plasma-derived lipid transfer protein with macrophages in culture. J Lipid Res 1988; 29: 1367-1377.
10. Benoist F, Lau P, McDonnell M, Doelle H, Milne R, McPherson R. Cholesteryl ester transfer protein mediates selective uptake of high density lipoprotein cholesteryl esters by human adipose tissue. J Biol Chem 1997; 272: 23572-23577. [DOI:10.1074/jbc.272.38.23572]
11. Ravnik SE, Zarutskie PW, Muller CH. Purification and characterization of a human follicular fluid lipid transfer protein that stimulates human sperm capacitation. Biol Reprod 1992; 47: 1126-1133. [DOI:10.1095/biolreprod47.6.1126]
12. Masson D, Drouineaud V, Moiroux P, Gautier T, Dautin G, Schneider M, et al. Human seminal plasma displays significant phospholipid transfer activity due to the presence of active phospholipid transfer protein. Mol Hum Reprod 2003; 9: 457-464. [DOI:10.1093/molehr/gag062]
13. Ata B, Yakin K, Balaban B, Urman B. GnRH agonist protocol administration in the luteal phase in ICSI-ET cycles stimulated with the long GnRH agonist protocol: a randomized, controlled double blind study. Hum Reprod 2008; 23: 668-673. [DOI:10.1093/humrep/dem421]
14. Trounson AO, Gardner DK. Hand book of in vitro fertilization. 2nd Ed. Florida; CRC press; 1999.
15. Brinsden PR, Hall B. A Textbook of In Vitro Fertilization and Assisted Reproduction: The Bourn Hall Guide to Clinical and Laboratory Practice. London; The Parthenon Publishing Group; 1999.
16. Masson D, Drouineaud V, Moiroux P, Gautier T, Dautin G, Schneider M, et al. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993; 8: 1061-1066. [DOI:10.1093/oxfordjournals.humrep.a138192]
17. Matsubayashi H, Sugi T, Arai T, Shida M, Kondo A, Suzuki T, et al. IgG-antiphospholipid antibodies in follicular fluid of IVF-ET patients are related to low fertilization rate of their oocytes. Am J Reprod Immunol 2006; 55: 341-348. [DOI:10.1111/j.1600-0897.2006.00374.x]
18. Von Wald T, Monisova Y, Hacker MR, Yoo SW, Penzias AS, Reindollar RR, et al. Age-related variations in follicular apolipoproteins may influence human oocyte maturation and fertility potential. Fertil Steril 2010; 93: 2354-2361. [DOI:10.1016/j.fertnstert.2008.12.129]
19. Boekholdt SM, Sacks FM, Jukema JW, Shepherd J, Freeman DJ, McMahon AD, et al. Cholesteryl ester transfer protein TaqIB variant, high-density lipoprotein cholesterol levels, cardiovascular risk, and efficacy of pravastatin treatment: individual patient meta-analysis of 13,677 subjects. Circulation 2005; 111: 278-287. [DOI:10.1161/01.CIR.0000153341.46271.40]
20. Collet X, Tall AR, Serajuddin H, Guendouzi K, Royer L, Oliveira H, et al. Remodeling of HDL by CETP in vivo and by CETP and hepatic lipase in vitro results in enhanced uptake of HDL CE by cells expressing scavenger receptor B-I. J Lipid Res 1999; 40: 1185-1193.
21. Kaji K, Kudo A. The mechanism of sperm-oocyte fusion in mammals. Reproduction 2004; 127: 423-429. [DOI:10.1530/rep.1.00163]
22. Ito K, Ishikawa F, Kanno T, Ishikawa Y, Akasaka Y, Akishima Y, et al. Expression of cholesteryl ester transfer protein (CETP) in germinal centre B cells and their neoplastic counterparts. Histopathology 2004; 45: 73-81. [DOI:10.1111/j.1365-2559.2004.01905.x]
23. Balestrieri M, Cigliano L, Simone ML, Dale B, Abrescia P. Haptoglobin inhibits lecithin-cholesterol acyltransferase in human ovarian follicular fluid. Mol Reprod Dev 2001; 59: 186-191. [DOI:10.1002/mrd.1021]
24. Hochberg RB. Biological esterification of steroids. Endocr Rev 1998; 19: 331-348. [DOI:10.1210/er.19.3.331]
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