Volume 2, Issue 2 (7-2004)                   IJRM 2004, 2(2): 58-64 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khosrowbeygi A, Zarghami N, Deldar Y. Correlation between Sperm Quality Parameters and Seminal Plasma Antioxidants Status. IJRM 2004; 2 (2) :58-64
URL: http://ijrm.ir/article-1-24-en.html
Correlation between Sperm Quality Parameters and Seminal Plasma Antioxidants Status
Ali Khosrowbeygi * , Nosratollah Zarghami , Yaghoub Deldar
Abstract:   (3064 Views)
Background: There is growing evidence that damage to spermatozoa by reactive oxygen species (ROS) play a key role in male infertility. Objective: The aim of the present study was to assess the antioxidant status of seminal plasma by measuring total antioxidant capacity (TAC) and activities of catalase and superoxide dismutase (SOD) in men with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia compared to normozoospermic males, and their correlations with seminal parameters. Materials and Methods: 46 men with seminal parameters abnormalities divided in three categories: asthenozoospermic (n=15), asthenoteratozoospermic (n=16) and oligoasthenoteratozoospermic (n=15), according WHO criteria, participated in the study. The control group consisted of 25 males with normozoospermia. Catalase activity was measured by Aebi spectrophotometeric method. Commercially available colorimetric assays were used for measuring SOD activity and TAC. Results: TAC evaluation showed significantly lower values in the total case group (n=46) versus control group (1.05±0.04 mmmol/ml vs. 1.51±0.07 mmol/ml, p<0.05). Catalase activity also showed significantly lower values in the total case group (n=46) versus control group (14.40± 0.93 U/ml vs. 21.33±1.50 U/ml). But this difference was not significant for SOD activity (5.31±0.56 U/ml vs. 6.19±0.83 U/ml). Both catalase activity and TAC in asthenozoospermic, asthenoteratozoospermic, oligoasthenoteratozoospermic subjects were significantly lower than normozoospermic males, but SOD activity did not show a significant difference between these groups. Both catalase activity and TAC showed a positively significant correlation with progressively motile sperms and normal sperm morphology, but these correlations with SOD activity were not significant. Conclusion: Decreasing seminal plasma antioxidant status especially catalase activity and TAC may have significant role in etiology of sperm abnormality. Article
Keywords: Seminal plasma, Total antioxidant capacity, Superoxide dismutase, Catalase
Full-Text [PDF 253 kb]   (952 Downloads) |   |   Full-Text (HTML)  (453 Views)  
Type of Study: Original Article |
References
1. Aebi H. Catalase in vitro. (1984) Methods Enzymol 105:121-126. [DOI:10.1016/S0076-6879(84)05016-3]
2. Agarwal A., Saleh R.A. (2002) Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am 29: 817-827. [DOI:10.1016/S0094-0143(02)00081-2]
3. Agarwal A., Saleh R.A., Bedaiwy M.A. (2003) Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 79: 829-843. [DOI:10.1016/S0015-0282(02)04948-8]
4. Agarwal A., Nallella K.P., Allamaneni S.S., Said T.M. (2004) Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online 8: 616-627. [DOI:10.1016/S1472-6483(10)61641-0]
5. Alkan I., Simsek F., Haklar G., Kervancioglu E., Ozveri H., Yalcin S., Akdas A. (1997) Reactive oxygen species production by the spermatozoa of patients with idiopathic infertility: relationship to seminal plasma antioxidants. J Urol 157:140-3. [DOI:10.1016/S0022-5347(01)65307-2]
6. Calamera J., Buffone M., Ollero M., Alvarez J., Doncel G.F. (2003) Superoxide dismutase content and fatty acid composition in subsets of human spermatozoa from normozoospermic, asthenozoospermic, and polyzoospermic semen samples. Mol Reprod Dev 66: 422-430. [DOI:10.1002/mrd.10368] [PMID]
7. Conquer J.A., Martin J.B., Tummon I., Watson L., Tekpetey F. (1999) Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoospermic vs. asthenozoospermic males. Lipids 34: 793-799. [DOI:10.1007/s11745-999-0425-1] [PMID]
8. Dandekar S.P., Nadkarni G.D., Kulkarni V.S., Punekar S. (2002) Lipid peroxidation and antioxidant enzymes in male infertility. J Postgrad Med 48:186-189.
9. Esfandiari N., Sharma R.K., Saleh R.A., Thomas A.J. Jr., Agarwal A. (2003) Utility of the nitroblue tetrazolium reduction test for assessment of reactive oxygen species production by seminal leukocytes and spermatozoa. J Androl 24: 862-870. [DOI:10.1002/j.1939-4640.2003.tb03137.x] [PMID]
10. Fraczek M., Szkutnik D., Sanocka D., Kurpisz M. (2001) Peroxidation components of sperm lipid membranes in male infertility. Ginekologia Polska 72: 73-79.
11. Fujii J., Iuchi Y., Matsuki S., Ishii T. (2003) Cooperative function of antioxidant and redox systems against oxidative stress in male reproductive tissues. Asian J Androl 5:231-242.
12. Garrido N., Meseguer M., Simon C., Pellicer A., Remohi J. (2004) Pro-oxidative and anti-oxidative imbalance in human semen and its relation with male fertility. Asian J Androl 6: 59-65.
13. Gil-Guzman E., Ollero M., Lopez M.C., Sharma R.K., Alvarez J.G., Thmas A.J. J.r., Agarval A. (2001) Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 16:1922-1930. [DOI:10.1093/humrep/16.9.1922] [PMID]
14. Hendin B.N., Kolettis P.N., Sharma R.K., Thomas A.J. Jr., Agarwal A. (1999) Varicocele is associated with elevated spermatozoal reactive oxygen species production and diminished seminal plasma antioxidant capacity. J Urol 161: 1831-1834. [DOI:10.1016/S0022-5347(05)68818-0]
15. Hsieh Y.Y., Sun Y.L., Chang C.C., Lee Y.S., Tsai H.D., Lin C.S. (2002) Superoxide dismutase activities of spermatozoa and seminal plasma are not correlated with male infertility. J Clin Lab Anal 16:127-131. [DOI:10.1002/jcla.10029] [PMID] [PMCID]
16. Keskes-Ammar L., Feki-Chakroun N., Rebai T., Sahnoun Z., Ghozzi H., Hammami, S. et al. (2003) Sperm oxidative stress and the effect of an oral vitamin E and selenium supplement on semen quality in infertile men. Arch Androl 49:83-94. [DOI:10.1080/01485010390129269] [PMID]
17. Koca Y., Ozdal O.L., Celik M., Unal S., Balaban N. (2003) Antioxidant activity of seminal plasma in fertile and infertile men. Arch Androl 49: 355-359. [DOI:10.1080/01485010390219728] [PMID]
18. Meucci E., Milardi D., Mordente A., Martorana G.E., Giacchi E., De Marinis L., Mancini A. (2003) Total antioxidant capacity in patients with varicoceles. Fertil Steril 3:1577-1583. [DOI:10.1016/S0015-0282(03)00404-7]
19. Mruk D.D., Silvestrini B., Mo M.Y., Cheng C.Y. (2002) Antioxidant superoxide dismutase - a review: its function, regulation in the testis, and role in male fertility. Contraception 65: 305-311. [DOI:10.1016/S0010-7824(01)00320-1]
20. Pasqualotto F.F., Sharma R.K., Nelson D.R., Thomas A.J. Jr., Agarwal A. (2000) Relationship between oidative stress, semen characteristics, and clinical diagnosis in men undergoing infertility investigation. Fertil Steril 73: 459-64. [DOI:10.1016/S0015-0282(99)00567-1]
21. Peeker R., Abramsson L., Marklund S.L. (1997) Superoxide dismutase isoenzymes in human seminal plasma and spermatozoa. Mol Hum Reprod 3:1061-1066. [DOI:10.1093/molehr/3.12.1061] [PMID]
22. Said T.M., Kattal N., Sharma R.K., Sikka S.C., Thomas A.J. Jr., Mascha E., Agarwal A. (2003) Enhanced chemiluminescence assay vs colorimetric assay for measurement of the total antioxidant capacity of human seminal plasma. J Androl 24: 676-680. [DOI:10.1002/j.1939-4640.2003.tb02726.x] [PMID]
23. Sanocka D., Kurpisz M. (2004) Reactive oxygen species and sperm cells. Reprod Biol Endocrinol 2:12. [DOI:10.1186/1477-7827-2-12] [PMID] [PMCID]
24. Shibahara H., Suzuki T., Obara H., Hirano Y., Onagawa T., Taneichi A., Takamizawa S., Sato I. (2002) Accuracy of the normal sperm morphology value by Sperm Quality Analyzer IIC: comparison with the strict criteria. Int J Androl 25: 45-48. [DOI:10.1046/j.1365-2605.2002.00322.x] [PMID]
25. Siciliano L., Tarantino P., Longobardi F., Rago V., De Stefano C., Carpino A. (2001) Impaired seminal antioxidant capacity in human semen with hyperviscosity or oligoasthenozoospermia. J Androl 22: 798-803.
26. Suzuki T., Shibahara H., Tsunoda H., Hirano Y., Taneichi A., Obara H., Takamizawa S., Sato, I. (2002) Comparison of the sperm quality analyzer IIC variables with the computer-aided sperm analysis estimates. Int J Androl 25: 49-54. [DOI:10.1046/j.1365-2605.2002.00324.x] [PMID]
27. Taylor C.T. (2001) Antioxidants and reactive oxygen soecies in human fertility. Environmental Toxicology and Pharmacology 10: 189-198. [DOI:10.1016/S1382-6689(01)00099-0]
28. Tkaczuk-Wlach J., Kankofer M., Jakiel G. (2002) Activity of superoxide dismutase and glutathione peroxidase in human semen in normozoospermia and spermatopathy. Ann Univ Mariae Curie Sklodowska 57: 369-375.
29. World Health Organization. (1999) WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interactin. 3erd edn Cambridge University Press, Cambridge, UK.
30. Zarghami N., Khosrowbeygi A. (2004) Evaluation of lipid peroxidation as an indirect measure of oxidative stress in seminal plasma. Iranian Journal of Reproductive Medicine 2: 34-39.
31. Zini A., Garrels K., Phang D. (2000) Antioxidant activity in the semen of fertile and infertile men. Urology 55: 922-926. [DOI:10.1016/S0090-4295(00)00453-2]
32. Zini A, Fischer MA, Mak V, Phang D, Jarvi K. (2002) Catalase-like and superoxide dismutase-like activities in human seminal plasma. Urol Res 30: 321-323. [DOI:10.1007/s00240-002-0283-0] [PMID]
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb