Volume 21, Issue 6 ( June 2023 2023)                   IJRM 2023, 21(6): 499-508 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rezaeilarijani S, Hosseini S M, Ekrami B. Study of different doses of zinc oxide nanoparticles by intraperitoneal and gavage methods on testicular tissue in Wistar rats: An experimental study. IJRM 2023; 21 (6) :499-508
URL: http://ijrm.ir/article-1-2564-en.html
1- Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran.
2- Department of Pathology, Babol Branch, Islamic Azad University, Babol, Iran. , dr.m.hosseini2323@gmail.com
3- Department of Animal Science Chaloos Branch, Islamic Azad University, Babol, Iran
Abstract:   (576 Views)
Background: Zinc oxide nanoparticles (ZnO-NPS) are widely used in human life; however, they do have side effects on human health. 
Objective: This study aimed to evaluate the different doses of ZnO-NPS on testicular tissue.
Materials and Methods: 35 male Wistar rats (10-12 wk, 220 ± 20 gr) were divided into 7 groups of 5, including the control group (gavaged distilled water daily), sham group (received intraperitoneal doses of distilled water twice a week). The group received intraperitoneal ZnO-NPS (25, 50, and 100 mg/kg body weight, twice a week), and gavage (150 and 200 mg/kg body weight daily). All stages of the test were performed in 4 wk then serum testosterone and tissue malondialdehyde, and ferric reducing antioxidant power levels were measured, also testes histopathological evaluation was performed.
Results: Our results showed that a reduced cell population of spermatozoa was observed in the group that received 25 mg/kg ZnO-NPS, while a reduced cell population of spermatozoa, edema, hyperemia, and vacuolar degeneration were observed in the group that received 50 and 100 mg/kg ZnO-NPS. The maximum amount of lesions were observed in the dose of 200 mg/kg. The highest amount of ferric reducing antioxidant power and testosterone levels were observed in the control group. Also, a 100 mg/kg intraperitoneal dose of ZnO-NPS and 150 mg/kg oral dose of ZnO-NPS were suitable doses to create a model of male genital lesions.
Conclusion: Nanoparticles are harmful factors for the reproductive system and consequently affect infertility, which requires the toxicity of the concentration of these nanoparticles to be evaluated and controlled.
Full-Text [PDF 3566 kb]   (508 Downloads) |   |   Full-Text (HTML)  (97 Views)  
Type of Study: Original Article | Subject: Reproductive Pattology

References
1. Adamu CI, Nganje TN, Edet A. Heavy metal contamination and health risk assessment associated with abandoned barite mines in Cross River State, southeastern Nigeria. Environ Nanotechnol Monit Manag 2014; 3: 10-21. [DOI:10.1016/j.enmm.2014.11.001]
2. Kołodziejczak-Radzimska A, Jesionowski T. Zinc oxide-from synthesis to application: A review. Materials 2014; 7: 2833-2881. [DOI:10.3390/ma7042833] [PMID] [PMCID]
3. Gatoo MA, Naseem S, Arfat MY, Mahmood Dar A, Qasim K, Zubair S. Physicochemical properties of nanomaterials: Implication in associated toxic manifestations. Biomed Res Int 2014; 2014: 498420. [DOI:10.1155/2014/498420] [PMID] [PMCID]
4. Hosseini SM, Moshrefi AH, Amani R, Razavimehr SV, Aghajanikhah MH, Sokouti Z, et al. Subchronic effects of different doses of Zinc oxide nanoparticle on reproductive organs of female rats: An experimental study. Int J Reprod BioMed 2019; 17: 107-118. [DOI:10.18502/ijrm.v17i2.3988] [PMID] [PMCID]
5. Nasrollahzadeh M, Sajadi SM. Risks of nanotechnology to human life. Interface Sci Technol 2019; 28: 323-336. [DOI:10.1016/B978-0-12-813586-0.00007-9]
6. Yao Y, Zang Y, Qu J, Tang M, Zhang T. The toxicity of metallic nanoparticles on liver: The subcellular damages, mechanisms, and outcomes. Int J Nanomedicine 2019; 14: 8787-8804. [DOI:10.2147/IJN.S212907] [PMID] [PMCID]
7. Baki ME, Miresmaili SM, Pourentezari M, Amraii E, Yousefi V, Spenani HR, et al. Effects of silver nano-particles on sperm parameters, number of Leydig cells and sex hormones in rats. Iran J Reprod Med 2014; 12: 139-144.
8. Zhang J, Xue H, Qiu F, Zhong J, Su J. Testicular spermatozoon is superior to ejaculated spermatozoon for intracytoplasmic sperm injection to achieve pregnancy in infertile males with high sperm DNA damage. Andrologia 2019; 51: e13175. [DOI:10.1111/and.13175] [PMID]
9. Haouas Z, Zidi I, Sallem A, Bhouri R, Ajina T, Zaouali M, et al., Reproductive toxicity of lead acetate in adult male rats: Histopathological and cytotoxic studies. J Cytol Histol 2015; 6: 293. [DOI:10.4172/2157-7099.1000293]
10. Salehi A, Hosseini SM, Kazemi S. Antioxidant and anticarcinogenic potentials of propolis for dimethylhydrazine-induced colorectal cancer in wistar rats. Biomed Res Int 2022; 2022: 8497562. [DOI:10.1155/2022/8497562] [PMID] [PMCID]
11. Wang J, Zhu X, Tan L, Zhao T, Ni Z, Zhang N, et al. Single and combined nanotoxicity of ZnO nanoparticles and graphene quantum dots against the microalga Heterosigma akashiwo. Environ Sci Nano 2022; 9: 3094-3109. [DOI:10.1039/D2EN00246A]
12. Hosseini SM, Hejazian LB, Amani R, Siahchehreh Badeli N. Geraniol attenuates oxidative stress, bioaccumulation, serological and histopathological changes during aluminum chloride-hepatopancreatic toxicity in male Wistar rats. Environ Sci Pollut Res Int 2020; 27: 20076-20089. [DOI:10.1007/s11356-020-08128-1] [PMID]
13. Mohammadi A, Kazemi S, Hosseini M, Najafzadeh Varzi H, Feyzi F, Morakabati P, et al. The chrysin effect in prevention of acetaminophen-induced hepatotoxicity in rat. Chem Res Toxicol 2019; 32: 2329-2337. [DOI:10.1021/acs.chemrestox.9b00332] [PMID]
14. Percie du Sert N, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker M, et al. The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. J Cereb Blood Flow Metab 2020; 40: 1769-1777. [DOI:10.1177/0271678X20943823] [PMID] [PMCID]
15. Budi HS, Opulencia MJC, Afra A, Abdelbasset WK, Abdullaev D, Majdi A, et al. Source, toxicity and carcinogenic health risk assessment of heavy metals. Rev Environ Health 2022; in press. [DOI:10.1515/reveh-2022-0096] [PMID]
16. Goma AA, Tohamy HG, El-Kazaz SE, Soliman MM, Shukry M, Elgazzar AM, et al. Insight study on the comparison between Zinc Oxide nanoparticles and its bulk impact on reproductive performance, antioxidant levels, gene expression, and histopathology of testes in male rats. Antioxidants 2020; 10: 41. [DOI:10.3390/antiox10010041] [PMID] [PMCID]
17. Taylor U, Barchanski A, Petersen S, Kues WA, Baulain U, Gamrad L, et al. Gold nanoparticles interfere with sperm functionality by membrane adsorption without penetration. Nanotoxicology 2014; 8 (suppl.): 118-127. [DOI:10.3109/17435390.2013.859321] [PMID]
18. Ahmed SM, Abdelrahman SA, Shalaby SM. Evaluating the effect of silver nanoparticles on testes of adult albino rats (histological, immunohistochemical and biochemical study). J Mol Histol 2017; 48: 9-27. [DOI:10.1007/s10735-016-9701-4] [PMID]
19. Atei Gh, Rezaei F, Khadem Abolfazli M. Why is gold forbidden for men in islam? An original study. Health, Spirituality and Medical Ethics 2015; 2: 11-14.
20. Abbasalipourkabir R, Moradi H, Zarei S, Asadi S, Salehzadeh A, Ghafourikhosroshahi A, et al. Toxicity of zinc oxide nanoparticles on adult male Wistar rats. Food Chem Toxicol 2015; 84: 154-160. [DOI:10.1016/j.fct.2015.08.019] [PMID]
21. Hosseini M, Amani R, Razavimehr V, Moshrefi AH, Aghajanikhah MH. [Istopathologic and biochemical study of zinc oxide nanoparticles effect on renal tissue in rats.] J Ilam Univ Med Sci 2018; 26: 177-186. (in Persian) [DOI:10.29252/sjimu.26.3.177]
22. Wang R, Song B, Wu J, Zhang Y, Chen A, Shao L. Potential adverse effects of nanoparticles on the reproductive system. Int J Nanomedicine 2018; 13: 8487-8506. [DOI:10.2147/IJN.S170723] [PMID] [PMCID]
23. Zhang L, Wei J, Duan J, Guo C, Zhang J, Ren L, et al. Silica nanoparticles exacerbates reproductive toxicity development in high-fat diet-treated Wistar rats. J Hazard Mater 2020; 384: 121361. [DOI:10.1016/j.jhazmat.2019.121361] [PMID]
24. Espanani HR, Shirani K, Sadeghi L, Yousefi bbabadi V, Amraei E. Investigation of the zinc oxide nanoparticles effect on testosterone, cholesterol and cortisol in rats. Res J Recent Sci 2014; 2277: 2502.
25. Hosseini SM, Amani R, Moshrefi AH, Razavimehr SV, Aghajanikhah MH, Sokouti Z. Chronic zinc oxide nanoparticles exposure produces hepatic and pancreatic impairment in female rats. Iran J Toxicol 2020; 14: 145-154. [DOI:10.32598/ijt.14.3.626.1]
26. Su L-J, Zhang J-H, Gomez H, Murugan R, Hong X, Xu D, et al. Reactive oxygen species-induced lipid peroxidation in apoptosis, autophagy, and ferroptosis. Oxid Med Cell Longev 2019; 2019: 5080843. [DOI:10.1155/2019/5080843] [PMID] [PMCID]
27. Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. The chemistry of reactive oxygen species (ROS) revisited: Outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. Int J Mol Sci 2021; 22: 4642. [DOI:10.3390/ijms22094642] [PMID] [PMCID]
28. Ismaël BM, Siaka S. The toxicity of environmental pollutants. In: Junqueira Dorta D, de Oliveira DP. The toxicity of environmental pollutants. Uk: IntechOpen; 2022.
29. Waris A, Sharif S, Naz S, Manzoor F, Jamil F, Hussain M, et al. Hepatotoxicity induced by metallic nanoparticles at the cellular level: A review. Environ Eng Res 2023; 28: 220625. [DOI:10.4491/eer.2022.625]
30. Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS, et al. Oxidative stress in human pathology and aging: Molecular mechanisms and perspectives. Cells 2022; 11: 552. [DOI:10.3390/cells11030552] [PMID] [PMCID]

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb