Volume 21, Issue 4 (April 2023)                   IJRM 2023, 21(4): 303-322 | Back to browse issues page

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Netam A K, Bhargava V P, Singh R, Sharma P. Testosterone propionate and Swarna Bhasma treatment modulated D-galactose induced reproductive alterations in male Wistar rats: An experimental study. IJRM 2023; 21 (4) :303-322
URL: http://ijrm.ir/article-1-2606-en.html
Testosterone propionate and Swarna Bhasma treatment modulated D-galactose induced reproductive alterations in male Wistar rats: An experimental study
Aashish Kumar Netam1 , Vikas Pankaj Bhargava1 , Rambir Singh2 , Poonam Sharma * 3
1- Department of Zoology, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India.
2- Department of Horticulture, Aromatic and Medicinal Plants, Mizoram University, Aizawl, Mizoram, India.
3- Department of Zoology, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India. , pnm245@yahoo.com
Abstract:   (696 Views)
Background: The male reproductive system undergoes several adverse age-related changes like decreased hormone synthesis, sperm count, and testicular alteration that can impact on fertility.
Objective: The study aims to investigate the effects of testosterone propionate (TP), and ayurvedic formulation Swarna Bhasma (SB) on D-galactose (D-gal) induced reproductive aging in male Wistar rats.
Materials and Methods: 60 male Wistar rats were divided into 10 groups of 6 animals. Reproductive aging was induced by D-gal (150 mg/kg Bwt) exposure for 60 days. The rats were then treated by post and combination treatment with TP (2 mg/kg Bwt) and SB (6.75 mg/kg Bwt). Then sperm parameters, reproductive hormones, inflammatory markers, testicular antioxidant enzymes, steroidogenic enzymes, and histological manifestation of testis were evaluated.
Results: Exposure of D-gal caused significant (p < 0.001) decrease in serum testosterone (T), testicular steroidogenic, and antioxidant enzymes. Administration of TP increased the serum T level, testicular antioxidant enzymes, and spermatogenic profile at a significant level of (p < 0.001) compared to D-gal. Further, the SB treatment significantly (p < 0.001) elevated the serum T level, sperm count, testicular antioxidant enzymes, steroidogenic enzymes, when compared to D-gal.
Conclusion: Both the treatment of TP and SB treatments recovered the reproductive impairments caused by D-gal. However, exogenous T supplementation via TP administration is associated with various side effects during long-term use. SB is an Ayurvedic formulation having a long history of usage in India. The current findings suggest that the SB may be used as a good alternative for potentiating reproductive function in aging males.

Keywords: D-galactose, Aging, Testosterone propionate, Swarna Bhasma, Testis, Hypogonadism, infertility.
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Type of Study: Original Article | Subject: Reproductive Biology
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