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Volume 10, Issue 2 (7-2012)
IJRM 2012, 10(2): 149-154 |
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Sahmani M, Najafipour R, Farzadi L, Sakhinia E, Darabi M, Shahnazi V, et al . Correlation between PPAR? protein expression level in granulosa cells and pregnancy rate in IVF program. IJRM 2012; 10 (2) :149-154
URL: http://ijrm.ir/article-1-261-en.html
URL: http://ijrm.ir/article-1-261-en.html
Mehdi Sahmani * 
1, Reza Najafipour2 , Laya Farzadi3 , Ebrahim Sakhinia1 , Masoud Darabi1 , Vahideh Shahnazi3 , Amir Mehdizadeh1 , Maghsod Shaaker1 , Mohammad Noori4
1, Reza Najafipour2 , Laya Farzadi3 , Ebrahim Sakhinia1 , Masoud Darabi1 , Vahideh Shahnazi3 , Amir Mehdizadeh1 , Maghsod Shaaker1 , Mohammad Noori4
1- Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Clinical Biochemistry and Molecular and Cellular Research Center, Ghazvin University of Medical Sciences, Qazvin, Iran
3- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
4- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2- Department of Clinical Biochemistry and Molecular and Cellular Research Center, Ghazvin University of Medical Sciences, Qazvin, Iran
3- Women’s Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
4- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract: (3153 Views)
Background: Peroxisome proliferative-activated receptors (PPARs) are nuclear receptors that involved in cellular lipid metabolism and differentiation. The subtype γ of the PPAR family (PPAR?) plays important roles in physiologic functions of ovaries.
Objective: To determine correlation between PPARγ protein level in granulosa cells and pregnancy rate in women undergoing in-vitro fertilization (IVF) treatment.
Materials and Methods: In this cross-sectional study, twenty-five samples of granulosa cells were collected from women referred to an IVF treatment center. PPARγ protein expression level in granulosa cells was determined in comparison with β-actin level as control gene with Western blot test. Laboratory pregnancy was determined by a rise in blood ?-hCG level fourteen days after embryo transfer. Correlation analyses were used to test for associations between the oocytes and pregnancy occurrence as outcome variables and PPARγ protein expression level.
Results: Correlation analysis indicated that there was no significant relationship between granulosa cells PPARγ protein level with IVF parameters including number of matured oocytes and the ratio of fertilized to matured oocytes. Comparison of granulosa cells PPARγprotein level with positive and negative laboratory pregnancy revealed also no significant relationship.
Conclusion: According to the results of this study, PPARγ protein level in granulosa cells could not be directly correlated to the success rate of IVF.
Objective: To determine correlation between PPARγ protein level in granulosa cells and pregnancy rate in women undergoing in-vitro fertilization (IVF) treatment.
Materials and Methods: In this cross-sectional study, twenty-five samples of granulosa cells were collected from women referred to an IVF treatment center. PPARγ protein expression level in granulosa cells was determined in comparison with β-actin level as control gene with Western blot test. Laboratory pregnancy was determined by a rise in blood ?-hCG level fourteen days after embryo transfer. Correlation analyses were used to test for associations between the oocytes and pregnancy occurrence as outcome variables and PPARγ protein expression level.
Results: Correlation analysis indicated that there was no significant relationship between granulosa cells PPARγ protein level with IVF parameters including number of matured oocytes and the ratio of fertilized to matured oocytes. Comparison of granulosa cells PPARγprotein level with positive and negative laboratory pregnancy revealed also no significant relationship.
Conclusion: According to the results of this study, PPARγ protein level in granulosa cells could not be directly correlated to the success rate of IVF.
Keywords: In-vitro fertilization, Peroxisome proliferative-activated receptor γ, Granulosa cells, Pregnancy rate.
Type of Study: Original Article |
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