Volume 19, Issue 5 (Suppl- 2021)                   IJRM 2021, 19(5): 22-22 | Back to browse issues page

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Dehghan Marvast L. K-22 Sperm selection using Zeta potential method. IJRM 2021; 19 (5) :22-22
URL: http://ijrm.ir/article-1-2740-en.html
K-22 Sperm selection using Zeta potential method
Dehghan Marvast L *
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract:   (351 Views)
In routine ICSI, the selection is based on morphology and viability, which does not necessarily preclude the chance injection of DNA-damaged or apoptotic sperm into the oocyte. Sperm with a high negative surface electrical charge, named “Zeta potential”, are mature and more likely to have intact chromatin. In this procedure, sperm is selected based on the presence of a negative charge on sperm membrane. Thus, the aim of our work is the comparison between pure gradient and Zeta method, to select spermatozoa with normal chromatin. Our aim is to develop a simple Zeta potential method for sperm isolation; and to analyze the sperm maturity, morphology, and DNA parameters.
Keywords: potential method, Zeta potential
Full-Text [PDF 131 kb]   (126 Downloads)    
Type of Study: Congress Abstract | Subject: Reproductive Genetics
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