Volume 10, Issue 3 (7-2012)                   IJRM 2012, 10(3): 223-228 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Khodadadi M, Basavaiah S, Abediankenari S. Effect of lithium chloride on the luteal steroidogenesis in gonadotropin-stimulated rat. IJRM 2012; 10 (3) :223-228
URL: http://ijrm.ir/article-1-279-en.html
Effect of lithium chloride on the luteal steroidogenesis in gonadotropin-stimulated rat
Maryam Khodadadi1 , Shiva Basavaiah1 , Saeid Abediankenari * 2
1- Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore-6, India
2- Department of Microbiology and Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , abedianlab@yahoo.co.uk
Abstract:   (3028 Views)
Background: Main function of corpus luteum is progesterone synthesis that is significantly accompanied with an increase in levels of mRNA encoding of steroidogenic enzymes known as luteal markers.
Objective: This study was designed to evaluate effects of lithium chloride on the release of steroid hormones and steroidogenic enzymes in gonadotropin-stimulated rats.
Materials and Methods: Immature 23 days old Wistar rats were divided into 10 groups; each group comprised of 8 rats, and induced with single injection of pregnant mare’s serum gonadotrophin (PMSG) and followed by single injection of human chorionic gonadotropin (hCG). Then, rats were given lithium chloride (LiCl) or saline at 12 hours post-hCG injection. Ovaries were collected in 4-hour interval from 8-24 hour post-hCG injection. Expression pattern of steroidogenic acute regulatory protein (StAR), side-chain cleavage cytochrome P450 (P450scc) and 3β-hydroxysteroid dehydrogenase (3β-HSD) genes were determined by semi-quantitative RT-PCR. In addition, serum levels of progesterone and 17β-estradiol were measured by ELISA.
Results: Our results showed that hCG stimulation of progesterone was markedly diminished and transcript levels of key steroidogenic enzymes were altered in the hormone-stimulated rats following LiCl treatment.
Conclusion: These results suggest that critical steps in the function of corpus luteum are disrupted by lithium. It is concluded that LiCl is an effective factor for suppressing of steroid genes expression.
Keywords: Luteal steroidogenesis, Lithium chloride, Corpus luteum, Progesterone, Estradiol
Full-Text [PDF 445 kb]   (651 Downloads) |   |   Full-Text (HTML)  (374 Views)  
Type of Study: Original Article |
References
1. Bachelot A, Beaufaron J, Servel N, Kedzia C, Monget P, Kelly PA, et al. Prolactin independent rescue of mouse corpus luteum life span: identification of prolactin and luteinizing hormone target genes. Am J Physiol Endocrinol Metab 2009; 297: 676-684. [DOI:10.1152/ajpendo.91020.2008]
2. Clark BJ, Wells J, King SR, Stocco DM. The purification, cloning, and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells. Characterization of the steroidogenic acute regulatory protein (StAR). J Biol Chem 1994; 269: 28314-28322.
3. Stocco C, Telleria C, Gibori G. The molecular control of corpus luteum formation, function, and regression. Endocr Rev 2007; 28: 117-149. [DOI:10.1210/er.2006-0022]
4. Baastrup PC. Lithium in the prophylactic treatment of recurrent affective disorders. Handbook of Lithium Therapy. MTP Press Limited, Lancaster, England; 1980: 26-38.
5. Jana D, Nandi D, Maiti R, Ghosh D. Effect of human chorionic gonadotrophin coadministration on the activities of ovarian [Delta] 5-3 [beta]-hydroxysteroid dehydrogenase, and 17 [beta]-hydroxysteroid dehydrogenase, and ovarian and uterine histology in lithium chloride-treated albino rats. Reprod Toxicol 2001; 15: 215-219. [DOI:10.1016/S0890-6238(01)00115-0]
6. Richards JS, Russell DL, Ochsner S, Espey LL. Ovulation: new dimensions and new regulators of the inflammatory-like response. Annu Rev Physiol 2002; 64: 69-92. [DOI:10.1146/annurev.physiol.64.081501.131029]
7. Yoshinaga K. Gonadotrophin-induced hormone secretion and structural changes in the ovary during the nonpregnant reproductive cycle. Handbook of Physiology 1973; 2: 363-388.
8. Albarracin CT, Gibori G. Prolactin action on luteal protein expression in the corpus luteum. Endocrinol 1991; 129: 1821-1830. [DOI:10.1210/endo-129-4-1821]
9. Sánchez-Criado JE, van der Schoot P, Uilenbroek JT. Evidence for luteotrophic and antiluteolytic actions of prolactin in rats with 5-day oestrous cycles. J Endocrinol 1988; 117: 455. [DOI:10.1677/joe.0.1170455]
10. Thakur SC, Thakur SS, Chaube SK, Singh SP. Subchronic supplementation of lithium carbonate induces reproductive system toxicity in male rat. Reprod Toxicol 2003; 17: 683-690. [DOI:10.1016/S0890-6238(03)00107-2]
11. Batürk M, Karaaslan F, Eel E, Sofuolu S, Tutu A. Effects of short and long-term lithium treatment on serum prolactin levels in patients with bipolar affective disorder. Prog Neuropsychopharmacol Biol Psychiatry 2001; 25: 315-322. [DOI:10.1016/S0278-5846(00)00165-2]
12. Khan I, Glaser LA, Gibori G. Reactivation of regressing corpora lutea by estradiol in the pregnant rat: dependence on placental lactogen. Biol Reprod 1987; 37: 1083. [DOI:10.1095/biolreprod37.5.1083]
13. Frasor J, Gibori G. Prolactin regulation of estrogen receptor expression. Trends Endocrinol Metabol 2003; 14: 118-123 [DOI:10.1016/S1043-2760(03)00030-4]
14. Niswender GD, Juengel JL, Silva PJ, Rollyson MK, McIntush EW. Mechanisms controlling the function and life span of the corpus luteum. Physio Rev 2000; 80: 1-29. [DOI:10.1152/physrev.2000.80.1.1]
15. Chen YJ, Feng Q, Liu YX. Expression of the steroidogenic acute regulatory protein and luteinizing hormone receptor and their regulation by tumor necrosis factor in rat corpora lutea. Biol Reprod 1999; 60: 419-427. [DOI:10.1095/biolreprod60.2.419]
16. Stocco DM, Wang XJ, Jo Y, Manna PR. Multiple signaling pathways regulating steroidogenesis and steroidogenic acute regulatory protein expression: more complicated than we thought. Mol Endocrinol 2005; 19: 2647-2659. [DOI:10.1210/me.2004-0532]
17. Mann L, Heldman E, Bersudsky Y, Vatner SF, Ishikawa Y, Almog O, et al. Inhibition of specific adenylyl cyclase isoforms by lithium and carbamazepine, but not valproate, may be related to their antidepressant effect. Bipolar Disorders 2009; 11: 885-896. [DOI:10.1111/j.1399-5618.2009.00762.x]
18. Hickey G, Oonk R, Hall P, Richards J. Aromatase cytochrome P450 and cholesterol side-chain cleavage P450 in corpora lutea of pregnant rats: diverse regulation by peptide and steroid hormones. Endocrinol 1989; 125: 1673-1682. [DOI:10.1210/endo-125-3-1673]
19. Martel C, Gagne D, Couet J, Labrie Y, Simard J, Labrie F. Rapid modulation of ovarian 3 [beta]-hydroxysteroid dehydrogenase/[Delta] 5-[Delta] 4 isomerase gene expression by prolactin and human chorionic gonadotropin in the hypophysectomized rat. Mol Cell Endocrinol 1994; 99: 63-71. [DOI:10.1016/0303-7207(94)90147-3]
20. Stocco CO, Chedrese J, Deis RP. Luteal Expression of Cytochrome P450 Side-Chain Cleavage, Steroidogenic Acute Regulatory Protein, 3-Hydroxysteroid Dehydrogenase, and 20 -Hydroxysteroid Dehydrogenase Genes in Late Pregnant Rats: Effect of Luteinizing Hormone and RU486. Biol Reprod 2001; 65: 1114. [DOI:10.1095/biolreprod65.4.1114]
21. Rekawiecki R, Nowik M, Kotwica J. Stimulatory effect of LH, PGE2 and progesterone on StAR protein, cytochrome P450 cholesterol side chain cleavage and 3 [beta] hydroxysteroid dehydrogenase gene expression in bovine luteal cells. Prost Other Lipid Mediat 2005; 78: 169-184. [DOI:10.1016/j.prostaglandins.2005.06.009]
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Designed & Developed by : Yektaweb