International Journal of
Reproductive Biomedicine
Tue, May 13, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 103-103 |
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Tork S, Molaeeghaleh N, Abdi S, Movassaghi S, Sharifi Z. O-2 Evaluation of the effects of human bone marrow mesenchymal stem cells conditioned medium on growth and maturation of mouse ovarian follicle after vitrification. IJRM 2021; 19 (5) :103-103
URL: http://ijrm.ir/article-1-2828-en.html
URL: http://ijrm.ir/article-1-2828-en.html
1- Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2- Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. ,sh.abdi@iautmu.ac.ir
2- Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. ,
Abstract: (248 Views)
Background: In vitro culture of isolated follicles of cryopreserved ovaries can be proposed as a fertility preservation method. MSCs secreting various levels of growth factors and are an appropriate option for enriching the follicle culture media.
Objective: The purpose of this study was to evaluate the effect of human bone marrow mesenchymal stem cells derived conditioned medium (hBMSCs-CM) on growth and maturation of mouse ovarian follicle, and embryonic development after vitrification.
Materials and Methods: hBMSCs were cultured and the collected conditioned medium was concentrated and stored. The collected ovaries from Two-wk-old mice were divided randomly into vitrified and non-vitrified groups. Preantral follicles of both groups were isolated and cultured in α-MEM enriched with ITS and FBS supplemented with different concentrations of CM (2.5, 5, and 7.5%) for 12 days. During the culture period, survival rate, follicular maturation, follicular diameter, and levels of 17 βestradiol secretion were evaluated. In vitro fertilization and embryonic development were observed after culture.
Results: The survival rate, antrum formation, and oocyte maturation were higher in 7.5% CM subgroups than 2.5 and 5% CM in both vitrified and non-vitrified groups. Also, the follicle diameter in 7.5% CM was higher than other subgroups of both groups on day 4. Higher percentages of fertilization and embryo development were seen in 7.5% CM subgroups of the non-vitrified and vitrified group. Also, higher hormone secretion was observed in 7.5% CM subgroup in both vitrified and non-vitrified groups.
Conclusion: The present study suggests that the addition of 7.5% CM to mice ovarian preantral follicle culture media enhances follicle growth and oocyte maturation.
Objective: The purpose of this study was to evaluate the effect of human bone marrow mesenchymal stem cells derived conditioned medium (hBMSCs-CM) on growth and maturation of mouse ovarian follicle, and embryonic development after vitrification.
Materials and Methods: hBMSCs were cultured and the collected conditioned medium was concentrated and stored. The collected ovaries from Two-wk-old mice were divided randomly into vitrified and non-vitrified groups. Preantral follicles of both groups were isolated and cultured in α-MEM enriched with ITS and FBS supplemented with different concentrations of CM (2.5, 5, and 7.5%) for 12 days. During the culture period, survival rate, follicular maturation, follicular diameter, and levels of 17 βestradiol secretion were evaluated. In vitro fertilization and embryonic development were observed after culture.
Results: The survival rate, antrum formation, and oocyte maturation were higher in 7.5% CM subgroups than 2.5 and 5% CM in both vitrified and non-vitrified groups. Also, the follicle diameter in 7.5% CM was higher than other subgroups of both groups on day 4. Higher percentages of fertilization and embryo development were seen in 7.5% CM subgroups of the non-vitrified and vitrified group. Also, higher hormone secretion was observed in 7.5% CM subgroup in both vitrified and non-vitrified groups.
Conclusion: The present study suggests that the addition of 7.5% CM to mice ovarian preantral follicle culture media enhances follicle growth and oocyte maturation.
Type of Study: Congress Abstract |
Subject:
Reproductive Epidemiology
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