International Journal of
Reproductive Biomedicine
Tue, Jul 1, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 104-104 |
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O-3 The effect of Diazinon on cell proliferation and apoptosis in testicular tissue of rats and the protective effect of vitamin E. IJRM 2021; 19 (5) :104-104
URL: http://ijrm.ir/article-1-2829-en.html
URL: http://ijrm.ir/article-1-2829-en.html
Abstract: (244 Views)
Background: Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agriculture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis.
Objective: This study aimed to examine the effect of diazinon on apoptosis and the proliferation of germ cells in rat testis and the protective effect of vit E.
Materials and Methods: In this experimental study, 30 male Wistar rats were divided into five groups (n = 6 per group) consisting of control, sham (received olive oil), experimental group I (60 mg/kg DZN), experimental group II (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group III (200 mg/kg Vit E). After six wks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL).
Results: Compared with the control group, DZN in the experimental group I decreased the number of PCNA positive cells and increased the number of TUNEL-positive cells (p < 0.001). Vit E improved detrimental changes by the decrease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (p < 0.001).
Conclusion: Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.
Objective: This study aimed to examine the effect of diazinon on apoptosis and the proliferation of germ cells in rat testis and the protective effect of vit E.
Materials and Methods: In this experimental study, 30 male Wistar rats were divided into five groups (n = 6 per group) consisting of control, sham (received olive oil), experimental group I (60 mg/kg DZN), experimental group II (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group III (200 mg/kg Vit E). After six wks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL).
Results: Compared with the control group, DZN in the experimental group I decreased the number of PCNA positive cells and increased the number of TUNEL-positive cells (p < 0.001). Vit E improved detrimental changes by the decrease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (p < 0.001).
Conclusion: Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.
Type of Study: Congress Abstract |
Subject:
Reproductive Epidemiology
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