International Journal of
Reproductive Biomedicine
Fri, Aug 22, 2025
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Volume 19, Issue 5 (Suppl- 2021)
IJRM 2021, 19(5): 227-227 |
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Hashemibeni B, Izadi MA, Valiani A, Esfandiari I, Bahramian H, Dortaj H et al . P-60 Investigation and comparison of the effect of TGF-β3, kartogenin and Avocado/Soybean unsaponifiables on the in-vitro and in-vivo chondrogenesis of human adipose derived stem cells on fibrin scaffold. IJRM 2021; 19 (5) :227-227
URL: http://ijrm.ir/article-1-2860-en.html
URL: http://ijrm.ir/article-1-2860-en.html
Hashemibeni B1 
, Izadi MA1 , Valiani A1 , Esfandiari I1 , Bahramian H1 , Dortaj H2 , Pourentezari M *3
, Izadi MA1 , Valiani A1 , Esfandiari I1 , Bahramian H1 , Dortaj H2 , Pourentezari M *3
1- Department of Anatomical Sciences and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran.
2- Department of Tissue Engineering and Applied Cell Science, Shiraz University of Applied Medical Science and Technologies, Shiraz, Iran.
3- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ,m.pourentezari@gmail.com
2- Department of Tissue Engineering and Applied Cell Science, Shiraz University of Applied Medical Science and Technologies, Shiraz, Iran.
3- Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ,
Abstract: (408 Views)
Background: There are lack of suitable therapeutic approaches to cartilage defect.
Objective: This study was designed to determine the effect of TGF-β3, avocado/soybean (ASU) and Kartogenin (KGN) on chondrogenic differentiation in human adipose-derived stem cells (hADSCs) on fibrin scaffold.
Materials and Methods: hADSCs seeded in fibrin scaffold and cultured in chondrogenic media. These cells divided in to 4 groups (control, TGF-β3, ASU and KGN). Cell viability was estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, or MTT assay, differentiated cells evaluated by histological and immunohistochemical techniques. Expression genes [sex determining region Y-box 9 (SOX9), Aggrecan (AGG), type II collagen (Coll II) and type X collagen (Coll X)] assessed by real-time PCR. For study on animal model, differentiated cells in fibrin scaffolds were subcutaneously transplanted in rats. Histological and immunohistochemistry was done in animal model.
Results: The results of the real-time PCR indicated that SOX9, AGG and Col II genes expression in TGF-β3, KGN and ASU groups were significantly higher (p < 0.01) compared to the control group, Col X gene expression only in TGF-β3 group was significantly higher (p < 0.01) compared to the control group. The glycosaminoglycan (GAG) deposition was higher in TGF-β3, KGN and ASU groups compared to the control group. The immunohistological analysis showed the distribution of collagen type X in the extracellular matrix in fibrin scaffold TGF-β3 group was significantly higher in control, KGN and ASU groups, (p < 0.001).
Conclusion: ASU, and in particular KGN was suitable for successful chondrogenic differentiation of hADSCs and a suppressor of the consequent hypertrophy.
Objective: This study was designed to determine the effect of TGF-β3, avocado/soybean (ASU) and Kartogenin (KGN) on chondrogenic differentiation in human adipose-derived stem cells (hADSCs) on fibrin scaffold.
Materials and Methods: hADSCs seeded in fibrin scaffold and cultured in chondrogenic media. These cells divided in to 4 groups (control, TGF-β3, ASU and KGN). Cell viability was estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, or MTT assay, differentiated cells evaluated by histological and immunohistochemical techniques. Expression genes [sex determining region Y-box 9 (SOX9), Aggrecan (AGG), type II collagen (Coll II) and type X collagen (Coll X)] assessed by real-time PCR. For study on animal model, differentiated cells in fibrin scaffolds were subcutaneously transplanted in rats. Histological and immunohistochemistry was done in animal model.
Results: The results of the real-time PCR indicated that SOX9, AGG and Col II genes expression in TGF-β3, KGN and ASU groups were significantly higher (p < 0.01) compared to the control group, Col X gene expression only in TGF-β3 group was significantly higher (p < 0.01) compared to the control group. The glycosaminoglycan (GAG) deposition was higher in TGF-β3, KGN and ASU groups compared to the control group. The immunohistological analysis showed the distribution of collagen type X in the extracellular matrix in fibrin scaffold TGF-β3 group was significantly higher in control, KGN and ASU groups, (p < 0.001).
Conclusion: ASU, and in particular KGN was suitable for successful chondrogenic differentiation of hADSCs and a suppressor of the consequent hypertrophy.
Type of Study: Congress Abstract |
Subject:
Pregnancy Health
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